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Affordable price & shipped at room temperature.
A premixed, ready-to-use solution for efficient amplification of DNA templates by PCR.
- 2X Eco-Taq MasterMix contains Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR, as well as an inert loading dye.
- This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. To prepare the final PCR, only primers and template DNA need to be added.
- The mix retains all features of Taq DNA Polymerase and can amplify DNA targets up to 5 kb (simple template).
- The elongation velocity is 0.9~1.2kb/min (70~75°C).
- It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'-dA overhang PCR product.
Features:
- Convenient: Just add primers and template DNA
- High yields of PCR products with minimal optimization.
- High efficiency: saves your time by simplifying the process
- Reproducible: lower contamination risk and pipetting error.
Applications:
- High-throughput PCR.
- Routine PCR with high reproducibility
- Generation of PCR products for TA cloning
Contents:
2X Taq Mix 1ml
Store at -20°C
For research use only
MIDSCI ELISA & Short Term
Specifications:
| Length | 146.05 mm |
| Length (w/ end tabs removed) | 119.05 mm |
| Width | 79.38 mm |
| Overall Thickness | 3.1 mil |
| Adhesive Thickness | 1.1 mil |
| Material | Polyester |
| Adhesive | Medical Grade Acrylic |
| Max. Temperature | 120 °C |
| Min. Temperature | -40 °C |
| Primary Usages | Elisa, Storage or Sample Cover |
Due to supply constraints 100-SEAL-PLT, STR-SEAL-PLT, XTR-100 and XTR-LG-100 are out of stock.
STR-SEAL-PLT alternative is GPS-PP-100.
PP-100 substrate is polypropylene instead of polyester for the STR-SEAL-PLT. GPS-PP-100 is designed for the same application – ELISA/Incubation/General Storage.
100-SEAL-PLT alternative is TTS-100.
XTR-100 and XTR-LG-100 alternatives are XTR2-100 & XTR2-LG-100.
ELISA, Incubation, and Short Term Incubation Sealing Films
All films on this page are designed for ELISA, Incubation, and Short Term Incubation Sealing Films. If you are looking for something specific please consult our selection guides by attribute or application on this page. All films are certified DNase- & RNase-Free.
Specific film descriptions:
1x8 MiniStrips (GP-CS1X8-400 and GPS-CS1X8-400) are less expensive than full-size sheets when using only a partial plate. Designed for use with either 1x8 strip-well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed. Ideal for COVID-19 testing to seal a row of 8 wells after swabs are deposited into a deep-well plate.
General Purpose (GP-PP-100 and GPS-PP-100) polypropylene lms oer superior barrier properties to minimize evaporation and prevent well-to-well contamination. Ideal for use with polypropylene plates.
Pre-scored lm (SP2-RL-500) on a roll is a more economical and ecient alternative to rapidly seal microplates or racked test tubes; faster than applying single sheeted lm. Self-standing, non-slip supports hold the pre-scored lm roll securely in place for quick and easy bench- top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn o or fed directly into a waste basket for disposal.
The SealMate SealPlate Starter Kit (SM-KIT-SP and replacement rolls) features a re-usable dispenser and two rolls of perforated lm. The dispenser facilitates the quick and easy dispensing of lm from a roll allowing microplates to be sealed three times faster than using sheeted lms. Cost-eective replacement rolls are sold separately. Ideal for use under the hood; the SealMate dispenser minimizes lm contamination.
SealPla te (100-SEAL-PLT and STR-SEAL-PLT) minimizes evaporation, prevents well-to-well contamination and spillage. Eliminates the edge eect which can occur with plate lids (e.g. greater evaporation in the outside plate wells).
SealPlate Color Tabs (SP-IDx-100) Each end-tab features a colored dot and white writing area. An inscribed color-coded end-tab can be removed and pasted into a laboratory notebook while the other remains on the plate for identication. The lm sheet can be removed from the plate without disturbing the identication label due to the nely perforated end-tabs. Choose from 5 dierent individual colors or the assortment pack.
SealPlate 2x8 MiniStrips (SP-2X8-50 and SPS-2X8-50) are less expensive than full-size sheets when using only a partial plate. Designed for use with either 1x8 strip- well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed.
ValueSeal (SP-VS-100) features smaller dimensions and a single non-perforated end-tab to provide a more cost-eective alternative to protect samples from evaporation, external contamination, dust and particulates.
Gel Cutting Tips
- Safely excise bands from gels
- Avoid cross contamination
- One-handed operation for quick and accurate gel cutting
- Safer than using razor blades and won't scratch transilluminators
- 1.1 x 4 mm
- Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
- RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
- DNase/RNase/Protease free
The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.
- Used for agarose electrophoresis of DNA, RNA or nucleic acids
- Contains 3 tracking dyes and 15% Ficoll in a special Tris dye
-
- Light blue - around 4000bp in 1% agarose
- Indigo - around 600bp in 1% agarose
- Magenta - around 150bp in 1% agarose
- DNase/RNase/Protease free
This brand is being discontinued, please contact us for other options.
Need great dNTPs at a great price? Try our PR1MA™ dNTPs
Bullseye dNTP Mix - 12.5 mM
- Mix of dATP, dCTP, dGTP, dTTP
- Each nucleotide is at a concentration of 12.5 mM
- Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
- High purity: >98% by HPLC
- Supplied in solution at pH 7.5
- dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
- Functionally tested with thermostable polymerases
This brand is being discontinued, please contact us for other options.
Need great dNTPs at a great price? Try our PR1MA™ dNTPs
Bullseye dNTP Mix - 10 mM
- Mix of dATP, dCTP, dGTP, dTTP
- Each nucleotide is at a concentration of 10 mM
- Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
- High purity: >98% by HPLC
- Supplied in solution at pH 7.5
- dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
- Functionally tested with thermostable polymerases
Please know that the manufacturer has suspended manufacturing this product indefinitely.
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
Sealing Mat for 96-Well PCR Plates
Reusable and versatile, Axygen AxyMats minimize evaporation in a wide range of temperatures in PCR and storage applications.
- Compact in Size: 102 x 136 x 104 mm
- Preprogramed Chip: with unlimited applications
- Touch Start: One Button to Go!
- LCD Display: for customers to define status easily!
- Portable: Only 1 kg
Qamp Mini is designed to achieve polymerase chain reaction (PCR) for the amplification of the targeted DNA in a miniature device making molecular biology more fun in the field. The Qamp Mini contains a centrally positioned Peltier heating and cooling module for 1-8 samples. The design leads to accuracy in analysis and cost efficiency without sacrificing performance and quality. With the compact size and One-Click to Go design Qamp Mini is the ideal instrument for laboratories or classrooms and in the fields of epidemiology, veterinary, food testing, pathogen detection, ecology, archaeology research and others.
Sample Number |
8 (2 x 4 wells) |
Temperature Range |
4°C - 99°C |
Max Heating Rate (°C/sec.) |
4.6°C |
Max Cooling Rate (°C/sec.) |
3.4°C |
Temperature Accuracy |
± 0.4°C |
Temperature Uniformity Across Block |
± 0.4°C |
Max No. of Cycles |
unlimited |
Max No. of Steps |
unlimited |
Display |
LCD |
Heated Lid |
105°C Fixed (pre-heat to 60°C) |
Dimensions (L x W x H) |
10 x 13 x 10 cm |
Weight |
1 Kg |
Power |
VAC 100-240, 50/60 Hz, 120 W |
Pfu 2x Master Mix
Pfu DNA Polymerase 2x master mix is ready to use premix which contains Pfu DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications. Pfu DNA Polymerase is a heat stable DNA polymerase which has 5' 3' DNA polymerase and 3' 5' exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. Pfu 2x Master Mix product is supplied with the unique Intact Genomics 5x Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Product Source
E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus.
Contents & Storage
- Pfu 2x master mix
- 5x Magic Enhancer
Store all contents at -20 °C.
1x Master Mix Composition
10 mM Tris-HCl pH 9.0, 50 mM KCl, 1.5 mM MgCl, 0.2 mM dNTPs, 5% Glycerol, 0.08% Igepal CA 630, 0.05% Tween-20, 100 Units/ml Pfu DNA Polymerase.
Protocol
1. Prepare a reaction mix according to the following table:
PCR reaction set up: |
|
Template DNA |
1-50 ng |
Forward primer (5 µM) |
1.0µl |
Reverse primer (5 µM) |
1.0µl |
Pfu 2x master mix |
10.0µl |
5x Magic Enhancer (optional) |
4.0µl |
HO up to |
20.0µl |
2. Mix the reaction mixture thoroughly.
3. Program the thermal cycler according to the manufacturer's instructions.
4. A typical PCR cycling program is outlined in the following table.
PCR cycling conditions: |
|||
Steps |
Temp. |
Time |
Cycles |
Initial Denaturation |
95 °C |
3 min |
1 |
Denaturation |
95 °C |
30 sec |
25-40 |
Annealing |
50-66 °C |
30 sec |
|
Extension |
72 °C |
1 min/kb |
|
Final Extension |
72 °C |
5 min |
1 |
Hold |
4-12 °C |
||
5. Place the PCR tubes in the thermal cycler and start the cycling program.
6. Analyze 5 µl of PCR products by agarose gel electrophoresis.
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