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PR1MA™ RNA Controls

 

Looking for safe assay controls for highly infectious viruses or foreign animal diseases?

 

MIDSCI™ offers a selection of PR1MA™ RNAs – nuclease-resistant, single-stranded RNAs, suitable as process controls for RNA extraction from various sample matrices. These specially engineered, non-infectious, MS2 phage-like particles protect their contents from degradation by nucleases and can package sequences of up to 1.5 kb from viruses such as SARS-CoV-2, foot-and-mouth disease virus, and human immunodeficiency virus.
 

Amount: Between 1E6 or 1E10 copies (cp)
Concentration: Between 1E7 or 1E10 cp / mL, respectively
Volume: 0.1 and 1 mL respectively
Lysis: 65° C for 5 minutes in the RT step or by standard RNA extraction
Storage temperature: 4°C

 

Buffer composition

  • 10 mM Tris HCl
  • 100 mM NaCl
  • 1 mM MgCl2
  • 0.1% gelatin
  • pH = 7.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

 

Item#:
ASPRIMARNACNTRL
 
Safety Sheet for PR1MA RNA Controls
Specs for PR1MA RNA Controls

Qamp Mini Thermal Cycler

  • Compact in Size: 102 x 136 x 104 mm
  • Preprogramed Chip: with unlimited applications
  • Touch Start: One Button to Go!
  • LCD Display: for customers to define status easily!
  • Portable: Only 1 kg
Qamp Mini is designed to achieve polymerase chain reaction (PCR) for the amplification of the targeted DNA in a miniature device making molecular biology more fun in the field. The Qamp Mini contains a centrally positioned Peltier heating and cooling module for 1-8 samples.  The design leads to accuracy in analysis and cost efficiency without sacrificing performance and quality.  With the compact size and One-Click to Go design Qamp Mini is the ideal instrument for laboratories or classrooms and in the fields of epidemiology, veterinary, food testing, pathogen detection, ecology, archaeology research and others. 


Specifications:

Sample Number8 (2 x 4 wells)
Temperature Range4°C - 99°C
Max Heating Rate (°C/sec.)4.6°C
Max Cooling Rate (°C/sec.)3.4°C
Temperature Accuracy± 0.4°C
Temperature Uniformity
Across Block		± 0.4°C
Max No. of Cyclesunlimited
Max No. of Stepsunlimited
DisplayLCD
Heated Lid105°C Fixed (pre-heat to 60°C)
Dimensions (L x W x H)10 x 13 x 10 cm
Weight1 Kg
PowerVAC 100-240, 50/60 Hz, 120 W
Item#:
ASQAMPMINI


PR1MA™ Bst DNA Polymerase

 

PR1MA™ Bst polymerase (patent pending) is a recombinant, truncated, thermostable Bacillus stearothermophilus DNA polymerase with high reverse transcriptase and strand-displacement activities, ideal for isothermal amplification of RNA and DNA targets. PR1MA™ Bst polymerase has increased sensitivity and speed relative to other Bst polymerases and can incorporate dUTP. 

 

Use PR1MA™ Bst polymerase to develop LAMP assays with high sensitivity and specificity.

  • Lacks 5’ to 3’ exonuclease activity
  • Only Bst polymerase in the market with robust RT and DNA polymerase activity
  • Thermostable, working temperature range 64 - 72°C
  • Tolerant to inhibitors
  • Storage temperature: - 20°C

Buffer composition

  • 50% glycerol
  • 50 mM Tris-HCl
  • 50 mM KCls
  • 1 mM DTT
  • 0.1  mM EDTA
  • 0.05% Tween – 20
  • 0.05% NP - 40 substitute
  • pH = 7.5

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.  
 

Item#:
ASPRIMABSTDNAP
 
Safety Sheet for PR1MA Bst DNA Polymerase
Specs for PR1MA Bst DNA Polymerase
Bulk, 250/pkg
  • Safely excise bands from gels
  • Avoid cross contamination
  • One-handed operation for quick and accurate gel cutting
  • Safer than using razor blades and won't scratch transilluminators

Cut gel with pipet tip on the end of a standard 1000µl pipettor. Gel piece is suspended in tip when pipettor is lifted from the gel. Expel the gel piece by pushing the pushbutton on the pipettor. Tip is ejected in normally using the ejector button.

Item#:
TGL-1140
Your Price:
117.40
Each
Mix, 40x50ul Rxns

This brand is being discontinued, please contact us for other options.


Looking for other alternatives?

Visit our PR1MA Polymerase page for more options! 
 


Bullseye High Fidelity PCR Master Mix


The newly developed Bullseye HFL PCR Master Mix represents the highest fidelity for a PCR product. Bullseye's HFL PCR Master Mix has eight times higher fidelity when compared to Pfu, previously considered to be the golden standard for high fidelity PCR. This Master Mix also works well for large PCR fragments. 

The Bullseye HFL PCR Master Mix is in a 2X format and contains modified, high fidelity thermal stable DNA polymerases, in a pre-optimized PCR buffer. The amplified DNA will be blunt-ended. When using this master mix for most PCR experiments, only template, primers and H2O will be needed.

SPECIAL FEATURES & APPLICATIONS
  • High fidelity with robustness
  • Large fragment PCR
  • 2X master mix - makes PCR easy and consistent
  • For cloning, promoter study, RACE, DNA sequencing and more
Item#:
BE135HFL
Your Price:
85.92
Each
This item is discontinued


PR1MA™ Taq DNA Polymerase 1X Master Mix

 

PR1MA™ DNA polymerase 1X Master Mix saves time and cost by enabling direct PCR amplification of unpurified templates. It contains a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays and is provided as a complete reaction master mix consisting of reaction buffer, dNTPs, MgCl2, and loading dye and only requires the addition of primers and DNA template. Once PCR is complete, the reaction products can be loaded directly into an agarose gel for analysis.

  • Lacks exonuclease activity
  • Thermotolerant up to 98°C
  • Inhibitor Resistant
  • Ideal for colony PCR, genotyping, and GC-rich templates
  • Storage temperature: -20°C 

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

 

Item#:
ASPRIMADNA1XMM
 
Safety Sheet for PR1MA Taq DNA polymerase 1X Master Mix
Specs for PR1MA Taq DNA polymerase 1X Master Mix
Adhesive Sealing Film, Polyolefin, 2.8 mil
These optically clear polyester films are ideally suited for Real Time PCR (qPCR). They feature an inert, ultra-clear, and strong encapsulated silicone adhesive that provides extraordinary performance in real-time PCR applications.

Encapsulated silicone greatly reduces the risk of sample contamination due to exposure to adhesive. Once the film is pressed onto the plate, the raised portion of the well penetrates the encapsulation and a tight seal is formed on just that surface (not over the sample). This silicone seal adheres aggressively and diminishes the possibility of "Edge Effect".

The plasticized backing of this film is scored on both ends to allow for two handed (precise) placement on the plate. Both film and adhesive maintain incredible clarity and are the choice for highly demanding manual and automated processes.

Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.
 

Specifications 

Length141.27 mm
Length (w/ end tabs removed)117.5 mm
Width77.8 mm
Overall Thickness2.8 mil
Adhesive Thickness2.0 mil
MaterialPolyester
AdhesiveEncapsulated Silicone
Max. Temperature120 °C
Min. Temperature-40 °C
Primary UsagesReal Time PCR (qPCR), PCR
Item#:
ASADHESIVEPOLFIN28
  • Quickly Spin down droplets and condensation
  • Use before and after thermal cycling to increase PCR yield
  • Accepts skirted, Non-Skirt and all standard PCR plates
  • Less than 1/4 the size of most plate centrifuges
  • 1 year warranty
Capacity: 2 PCR Plates
Rotor Speed: 2500 rpm
G Force: 500 xg
Rotor: Fixed Vertically
Dimensions (WxDxH): 7.5 x 8.2 x 7.2 in.
19 x 21 x 18.3 cm
Weight: 6 lbs. / 3 kg
Electrical: 120V or 230V, 50-60 Hz

 

Mini Plate Spinner Adapter Supports:

  • 8 x 0.2ml PCR Strips
  • 12 x 0.2ml PCR Strips
  • 0.2ml PCR Tubes
  • 48 well PCR Plates
Item#:
ASPCRCENT1

Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

Platemax CyclerSeal Sealing Film

  • Provides exceptional seal to safeguard samples during transport or storage
  • Eliminates well to well contamination and cross-over in PCR applications when used with Axygen's compression mat
  • Polypropylene film is wide temperature range compatible, -40° to 104°C
  • PCR-TS is designed for ELISA/EIA applications
  • PCR-TS-900 is twice as thick (as PCR-TS) and is suitable for water-bath and other difficult PCR applications
Item#:
ASPCRFILM5
  • Provides exceptional seal under the widest temperature conditions and reagents
  • Wide temperature range compatible, -80° to 104°C
  • Ideal for light sensitive samples and PCR or storage
  • Uniform adhesive provides optimum well sealing
  • PCR-AS-200 is pierceable
  • 100 films/pack
Item#:
ASPCRFILM4
For Sealing One Row of a 98-Well Plate

1x8 FilmStrips™ for Sealing Only One 8-well Row of a 96-well Plate

Designed for use with either 1×8 strip-well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed. Ideal for COVID-19 testing to seal a row of 8 wells after swabs are deposited into a deep-well plate.
  • Certified DNase- & RNase- free
  • A pliable polypropylene film minimizes aerosols upon removal
  • Each sheet contains 8 strips on a continuous liner for ease of use
  • Apply individually or two-at-a-time
  • Use as a secondary film to seal previously pierced wells or to prevent accidental pipetting
Applications
  • General laboratory use
  • Incubation
  • ELISA
Temperature Range
  • -40°C to +120°C
Item#:
AS1X8FILMSTRIPS
Need great (q)PCR Regents at a great price? Try PR1MA!  Click here to order.
 
 
  • Reduces formation of non-specific products
  • Improves results from multiplex reactions
  • Essential for low copy number
  • Buffer II: potassium/ammonium buffer for multiplex reactions
  • Activated at elevated temperatures
  • Gives higher specificity and greater yields than standard Taq
  • Chemical moiety is attached to the enzyme at the active site and renders it inactive at room temperature; is cleaved during a 15 min. heat activation step
  • Prevents mispriming during setup and the first ramp of thermal cycling
Item#:
ASPCRREAG9
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