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PR1MA™ Protein Markers
PR1MA™ All Blue, Regular Range Protein Markers
PR1MA™ All Blue, Regular Range Protein Markers are blue protein standards with 10 or 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, in the presence of Tris- Glycine buffer) are enhanced in intensity.
Loading Volume
- 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel
- 1.5~2.5 μL per well for general Western blotting transfer
The markers are supplied at a concentration of approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol)
PR1MA™ Tricolor Protein Markers
PR1MA™ Tricolor Protein Markers are three-color protein standards with 10, 12, or 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands, one green and one red band (at 25 kDa and 75 kDa, respectively, in the presence of Tris-Glycine buffer).
Loading Volume
- 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
- 1.5~2.5 μL per well for general Western blotting transfer.
The markers are supplied at a concentration of approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Specifications
Item# | Color | Bands | Range |
PR5010-BL | Blue | 10 | 9-180 kDA |
PR5011-BL | Blue | 11 | 9-180 kDA |
PR5010-TC | Tri-Color | 10 | 9-180 kDA |
PR5012-TC | Tri-Color | 12 | 9-245 kDA |
PR5013-TC | Tri-Color | 13 | 9-245 kDA |
- Plate Characteristics
- 96 x 0.1mL, Sub-skirted
- Low Profile (0.1mL), Frosted (low signal to noise ratio
- Extreme Uniformity (EU) Plate Design
- Uniform wall thickness ensures consistent even heating
- Thin wall-bottomed tube for rapid heat transfer
- Ideal paring with EU Caps with "Sealing Ring Band" Design
- Easy open - easy close with ergonomic caps: B57801, B79701, C79701
- Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
- Significant reduction in evaporation due to a wider sealing area.
- Certified DNA, DNase, RNA, RNase and pyrogen-free
Please give us a call for a sample!
Order# |
Description |
Non-Skirt |
|
B70501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs |
B70509 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs |
B50501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs |
AB70651 |
Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs |
Semi-Skirted |
|
B70651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs |
B70659 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs |
B50651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs |
B17480 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs |
B17489 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs |
Sub-Skirted |
|
ABI7501 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs |
ABI7509 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs |
AB19800-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs |
AB19809-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs |
Caps |
|
B57801 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs |
B79701 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs |
C79701 |
Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs |
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
- Standard thermal cycling and qPCR compatible
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Automation friendly, single notch corner, stackable
- Made from 99.9% virgin polypropylene
- 240ul well capacity (working capacity dependent on closure method)
- Barcoding available
- Certified DNAse/RNAse and pyrogen-safe
- Standard thermal cycling and qPCR compatible
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Single notch corner
- Made from 99.9% virgin polypropylene
- 340ul well capacity (working capacity dependent on closure method)
- Fits standard thermal cyclers, plus ABI PCR 2700, 2720, 9700, 9800, 7300, 7500, 7900, and ABI sequencing units
- Certified DNAse/RNAse and pyrogen-safe
PRYME PCR plastics are designed and optimized for PCR & qPCR applications.
Unique design and development provides maximum mold and manufacturing accuracy. We outline precise product specifications and tolerances and minimize mold cavities while using CYCLERtest's temperature control competence in our manufacturing process. Result: superior and reproducible products with, in case of (q)PCR vessels, an average wall thickness of 0.30 mm for tubes and strips and 0.35 mm for plates, both with a maximum tolerance of 0.05 mm. Combined with the identical design of (q)PCR product closure one ends up in fully interchangeable and freely selectable (q)PCR cap and vessel combinations.
Extreme uniform wall thickness leads to a very even heating of the sample and more homogeneous reaction conditions, leading to more reproducible results. Extreme uniform wall thickness also leads to lower evaporation rates and therefore more consistent (q)PCR results. Evaporation rates of >10% up to 30% significantly influence the ratio of your (q)PCR components in the vessel, and thus the resulting reaction and its result. In case of optical detection via optical reading through the side of the qPCR tubes, better wall uniformity increases the signal consistency.
Clever design and sealing bands around the tube closure points provide a leak-free seal, reducing evaporation and leading to more reproducible results. These superior sealing properties allow for running (q)PCR reactions with volumes as low as 5 µl. The cap design allows minimum pressure for closure and opening which is highly appreciated by users and minimizes RSI risks.
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
PCR Fast Plate, 96x0.1ml
- Optimized for fast PCR and qPCR compatible
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Made from 99.9% virgin polypropylene
- 240ul well capacity (working capacity dependent on closure method)
- Designed for ABI thermal cyclers 7500 and 7900, 9800, Veriti, and StepOne
- Certified DNAse/RNAse and pyrogen-safe
Please know that the manufacturer has suspended manufacturing this product indefinitely.
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Full-length insertion cap design prevents sample evaporation
- Made of 99.9% pure virgin polypropylene
- Certified DNAse/RNAse, and pyrogen-safe
PR1MA PCR Tool
Tool for opening and/or closing PCR strips or single tubes. Compatible with flat or domed caps. Can also be used for sealing film.
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
- Optimized for fast PCR and qPCR compatible
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Made from 99.9% virgin polypropylene
- 240ul well capacity (working capacity dependent on closure method)
- Designed for ABI thermal cyclers 7500 and 7900, 9800, Veriti, and StepOne
- Certified DNAse/RNAse and pyrogen-safe