OptiWell™ Square Well Sealing Mats

OptiWell™ Square Well Silicone Sealing Mats are designed to provide a secure, leak-proof seal across 24, 96, or 384 well plates, significantly reducing evaporation and sample loss. Ideal for use in PCR, high-throughput screening (HTS), and long-term sample storage, these sealing mats ensure consistent sample integrity and reliable data generation.

Manufactured from high-quality silicone, OptiWell™ Mats offer a tight, uniform seal that fits most standard plate formats, supporting uninterrupted lab workflows and reproducible results. Their durable, reusable design makes them a cost-effective solution for labs seeking efficiency and precision in every experiment.

Key Features

• Leak-proof seal for 24, 96, and 384 well plates
• Minimizes evaporation and sample loss
• Compatible with most major plate brands
• Ideal for PCR, HTS, and sample storage
• Made from durable, high-grade silicone
• Reusable and easy to apply

Azure Flash Western Chemiluminescence Kit

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

Each kit includes:

• 10x PVDF Membranes
• 100mL Chemi Blot Blocking Buffer
• 500μL Flash HRP Conjugate
• 250mL Flash Wash Buffer
• 100mL Radiance ECL Substrate

Features

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

• Fast protocol with few hands-on steps
• Antibody stabilization for enhanced chemi signal
• Low background and increased signal-to-noise ratios
• Increased detection sensitivity and linear dynamic range

OptiWell™ Round Well Sealing Mats

Reliable, Leak-Proof Seals for 96 and 384-Well Plates.

OptiWell™ Round Well Sealing Mats are designed to provide a secure, leak-proof seal for 96-well and 384-well round well plates, minimizing evaporation, contamination, and sample loss. Ideal for PCR, high-throughput screening (HTS), and sample storage, these reusable mats ensure consistent sample integrity and reliable data quality.

Manufactured from durable, high-performance materials, OptiWell™ sealing mats create a tight, uniform seal across every well—ensuring uninterrupted workflows and preventing cross-contamination. Their universal fit makes them compatible with most standard round well microplates, including OptiWell™ and other leading brands.

Available in multiple formats to suit various plate configurations, these mats are an essential addition to any high-throughput laboratory setting that demands precision, efficiency, and reproducibility.

Key Features

• Compatible with 96 and 384 round well plates
• Leak-proof seal – prevents evaporation and sample loss
• Ideal for PCR and HTS workflows – protects sample integrity
• Reusable and durable – long-lasting material supports repeated use
• Universally compatible – fits most standard round well plate brands
• Supports high-quality, consistent results – minimizes contamination and variability

Accuris Fast Extraction PCR Kit

The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

• Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
• Compatible with animal tissue, plant, saliva, & bacterial samples
• Extraction & Amplification in less than 60 minutes
• Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
• Process extracted sample via PCR with included mastermix
• Alternatively, amplify via qPCR (qPCR mastermix not included) 

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria
 

Proudly made in the USA! Click here for more made in America products!
  

Accuris SmartBlue™ Transilluminators

Accuris SmartBlue™ Transilluminators have been developed for viewing DNA gels stained with new, safe fluorescent dyes.  These new dyes, such as SmartGlow™, SYBR Green, Gel Green, have an excitation wavelength of 465-470 nm (visible blue light), and are safer to use than Ethidium Bromide.

• Blue light, safe for nucleic acids
• Uniform illumination (UI Technology)
• Optimal wavelength (465nm) for common safe stains
• Adjustable position, amber filter covers
• Optional amber viewing glasses: E4000-VG1

Safer for samples, safer for you: Accuris SmartBlue™ Transilluminators are the perfect partner for the most common safe DNA stains. These instruments emit blue light at a wavelength of 465 nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few.  SmartBlue™ transilluminators can also work for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images.   Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures.  In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.*

Adjustable filter cover: Both the E4101 and SB4500 transilluminators come with emission filter covers. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescing DNA bands to be seen clearly.  The covers can be set to a flat position for general gel viewing or can be set at an angle to provide easy access to the gel for band excision.  The SB4500 has a two-position filter cover, and the E4101 features a hinged filter cover.

Optional amber viewing glasses: Our E4000-VG1 SmartBlue™ Plus Viewing Glasses offer efficient blocking of the blue light wavelength from the transilluminators, allowing clear visualization of the fluorescing DNA bands without the filter cover in place.  These convenient glasses are recommended for users that are frequently excising DNA bands from gels.  SmartBlue™ Plus glasses also block 100% of UV wavelength so they can also be used for viewing gels on a UV transilluminator.

UI technology, bright and clear images: The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue™ Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.

Reliable, durable, and long-lasting: An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue™ Transilluminators. Unlike those in a UV transilluminator, the filters in the SmartBlue™ systems will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue™ Transilluminators are covered by a 2 year warranty.

* SmartBlue™ products use high intensity LED lights.  Although the visible blue wavelength is significantly safer than UV light, it is recommended to not stare directly at the viewing surface without the amber cover in place. 

Specifications 

Light Source	High Intensity Blue LEDs
Emission Filter Cover	Amber
Wavelength	Peak at 465 nm
E4000 Viewing Surface	17 x 12 cm
E4000 Ext. Dimensions	30.5 x 21.5 x 5 cm
E4101 Viewing Surface	10.5 x 10.5 cm
E4101 Ext. Dimensions	17 x 12 x 5 cm
Electrical	100 to  240V, 50-60Hz
E4000 Weight	1.2kg / 2.6lbs
E4101 Weight	0.34kg / 0.75lbs
Warranty	2 years

 
 Item # SB4500 is proudly made in the USA! Click here for more made in America products!
 

Radiance ECL Chemiluminescent Substrate

Chemiluminescent HRP substrate for chemiluminescent Western blots

Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.

Size: 500 mL

Features

• Performance — compare performance to SuperSignal West Pico and Amersham ECL
• Sensitivity — high sensitivity in low picograms
• Duration — Signal duration 6-8 hours
• Chemiluminescent Western Blotting
• The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.   

Radiance Plus Chemiluminescent Substrate

The most sensitive HRP substrate available for chemiluminescent Western blotting.

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

Size: 150 mL

Features

• Low-abundance protein detection
• Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
• Sensitivity in low femtograms
• Signal duration 6-8 hours   

Hoefer® Nitrocellulose Membranes

• Ideal for Western Blotting and other immunoblotting techniques
• BSA protein binding capacity of 160 µg/cm2–can bind a wide range of fragment sizes and can still be easily blocked for low background
• High wick rates (lateral flow)–substances move quickly through the membrane

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Radiance Q Chemiluminescent Substrate

Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.

Size: 150 mL

Features
 

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

• Extended dynamic range for detecting low and high abundance proteins within the same blot
• Compare performance to SuperSignal West Dura and Amersham ECL Prime
• Sensitivity in mid femtograms
• Signal duration 10-24 hours   

Hoefer® PVDF (Polyvinylidene Fluoride) Membranes

• Ideal for use in protein binding applications such as western blots, solid phase assays, and immunoblotting procedures
• Multiple reprobings possible
• Superior strength–can withstand aggressive handling or be used with automated equipment without breaking or tearing
• Low extractables–ensures tests will be clean with consistent results
• Exceptional sensitivity detects low-level components
• Hydrophobic–resists water
• High binding capacity–binds a wide range of fragment sizes

Proudly made in the USA! Click here for more made in America products!
  

BluBlot™ HS Autoradiography Film for Western Blots

BluBlot™ HS Autoradiography Film is a premium, high-sensitivity film specifically designed for Western blotting, chemiluminescence detection, and a broad range of autoradiographic applications.

Manufactured with a medium/dark blue polyester base and a double-emulsion coating, this film delivers sharp, publication-quality images with exceptional clarity and contrast.

Compatible with a wide range of radioisotopes, including ¹⁴C, ³²P, ³³P, ¹²⁵I, and ³⁵S, BluBlot™ HS ensures high-resolution results and superior signal-to-noise ratios, making it ideal for sensitive detection methods such as chemiluminescence, fluorescence, and phosphor imaging.

This film performs reliably with both manual development techniques and automatic X-ray film processors, offering outstanding versatility in the lab. It is also suitable for use with blue-emitting rare-earth screens and calcium tungstate intensifying screens. Packaged in light-safe, lined dispenser boxes of 100 sheets, BluBlot™ HS is protected for long-term storage and offers a minimum 18-month shelf life from the date of production. It provides performance comparable to leading autoradiography films at a fraction of the cost.

Key Features

• High-sensitivity autoradiography film for Western blots and electrophoretic applications
• Compatible with ¹⁴C, ³²P, ³³P, ¹²⁵I, ³⁵S isotopes
• Optimized for chemiluminescence and radioactive detection
• Medium/dark blue base for enhanced image contrast
• Double emulsion for superior clarity and consistency
• Publication-quality images
• Works with automatic processors and manual development
• Long shelf life (18+ months)
• Cost-effective alternative to premium autoradiography films