Azure Flash Western Chemiluminescence Kit

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

Each kit includes:

• 10x PVDF Membranes
• 100mL Chemi Blot Blocking Buffer
• 500μL Flash HRP Conjugate
• 250mL Flash Wash Buffer
• 100mL Radiance ECL Substrate

Features

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

• Fast protocol with few hands-on steps
• Antibody stabilization for enhanced chemi signal
• Low background and increased signal-to-noise ratios
• Increased detection sensitivity and linear dynamic range

Radiance ECL Chemiluminescent Substrate

Chemiluminescent HRP substrate for chemiluminescent Western blots

Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.

Size: 500 mL

Features

• Performance — compare performance to SuperSignal West Pico and Amersham ECL
• Sensitivity — high sensitivity in low picograms
• Duration — Signal duration 6-8 hours
• Chemiluminescent Western Blotting
• The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.   

Radiance Plus Chemiluminescent Substrate

The most sensitive HRP substrate available for chemiluminescent Western blotting.

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

Size: 150 mL

Features

• Low-abundance protein detection
• Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
• Sensitivity in low femtograms
• Signal duration 6-8 hours   

Radiance Q Chemiluminescent Substrate

Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.

Size: 150 mL

Features

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

• Extended dynamic range for detecting low and high abundance proteins within the same blot
• Compare performance to SuperSignal West Dura and Amersham ECL Prime
• Sensitivity in mid femtograms
• Signal duration 10-24 hours   

PR1MA™ Tn5 2.0 Transposase

PR1MA™ Tn5 2.0 Transposase (Tnp) is a hyperactive retroviral integrase engineered for improved activity, speed, and robustness that is used to construct random next-generation sequencing libraries and to study chromatin structure using targeted ATAC-seq. PR1MA™ Tn5 2.0 can be used to randomly fragment any target and insert unique oligonucleotide adapters in a single reaction which reduces the time and sample requirements relative to traditional next-gen sequencing library construction. 

• Optimal temperature: 55°C
• Inactivation: 40X Stop solution included (2% SDS)
• Storage temperature: -20°C
• 10X Tn5 reaction buffer included

Buffer composition

• 100 mM Tris-HCl
• 100 mM MgCl2
• pH = 7.5

*These products are intended for research use only, not for diagnostic use. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.   
 

2025 LabValues Promotion!

For a limited time enjoy discounted pricing on the

ENDURO™ Power Supply Series (E0303 and E0203)!

 

See flyer for more details.

 

Special price applies only to the specified item; no substitutions.

Cannot be combined with other discounts or pricing. Not valid on prior purchases.

Promo is valid 01/06/2025 – 06/30/2025 or while supplies last.

All terms and conditions apply.

ENDURO™ Power Supply 

• Two models (300V and 250V) available
• For DNA, RNA, or protein electrophoresis, and blotting applications
• Constant voltage or constant current with automatic crossover
• 250 V model also offers a constant power operating mode
• Timed or continuous operation
• Short circuit and over current/ over voltage protection
• No load and load change detection system
• Programming includes option for automatic recovery after power failure
• 3 year warranty

The new Enduro Power Supplies meet a wide range of applications including DNA, RNA, and protein electrophoresis, blotting, and many others. Two models are available with voltage ranges to 300V and current ranges to 3000mA. Operating modes include constant voltage or constant current with automatic crossover. In addition, the 250V model offers a constant power operating mode.

All parameters, including voltage, current, power (250V model only), and time are easily set using the membrane keypad and displayed digitally on the back lit LCD. Compact in design with a small footprint, the Enduro Power Supplies are also stackable to save space. For easy viewing and setting of parameters on the bench or on a shelf, the front of the power supply can be raised with the integral folding feet.

Specifications

PR1MA™ Taq DNA Polymerase

Saves time and cost by enabling direct PCR amplification of unpurified templates. PR1MA™ Taq DNA polymerase is a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays. It is supplied with 4 M betaine to improve amplification of GC-rich DNA and 30% sucrose to improve amplification from inhibitor-rich substrates such as blood.

• Lacks exonuclease activity
• Thermotolerant up to 98°C
• Resistant to inhibitors, e.g., whole blood
• Ideal for GC-rich templates
• Storage temperature: -20°C
• 10X PR1MA™ buffer, 4 M Betaine, and 30% Sucrose included

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• 0.5% Tween-20
• 0.5% NP-40 substitute
• pH = 7.5 

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

PR1MA™ CRISPR/Cas9 Nucleases

The RNA-guided endonuclease Cas9, associated with Type II CRISPR/Cas systems, site-specifically digests DNA using a single guide RNA (sgRNA) which it binds to direct it to the complementary sequence. MIDSCI™ offers two traditional versions of Streptococcus pyogenes Cas9 nuclease: CRISPR/Cas9, ideal for in vitro DNA digestion, and CRISPR/Cas9 NLS, for in vivo nuclear localization.

• Optimal temperature: 37°C
• Heat inactivation: 65°C for 20 minutes
• Storage temperature: -20°C

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

PR1MA™ Bst DNA Polymerase

PR1MA™ Bst polymerase (patent pending) is a recombinant, truncated, thermostable Bacillus stearothermophilus DNA polymerase with high reverse transcriptase and strand-displacement activities, ideal for isothermal amplification of RNA and DNA targets. PR1MA™ Bst polymerase has increased sensitivity and speed relative to other Bst polymerases and can incorporate dUTP. 

Use PR1MA™ Bst polymerase to develop LAMP assays with high sensitivity and specificity.

• Lacks 5’ to 3’ exonuclease activity
• Only Bst polymerase in the market with robust RT and DNA polymerase activity
• Thermostable, working temperature range 64 - 72°C
• Tolerant to inhibitors
• Storage temperature: - 20°C

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCls
• 1 mM DTT
• 0.1  mM EDTA
• 0.05% Tween – 20
• 0.05% NP - 40 substitute
• pH = 7.5

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.  
 

2025 LabValues Promotion!
For a limited time enjoy discounted pricing on select
Labnet Enduro™ UV Transilluminators (U1001 and U1002)!

 

See flyer for more details.

 

Special price applies only to the specified item; no substitutions.

Cannot be combined with other discounts or pricing. Not valid on prior purchases.

Promo is valid 01/06/2025 – 06/30/2025 or while supplies last.

All terms and conditions apply.

Enduro™ UV Transilluminators

PR1MA™ Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers are blue protein standards with 10 or 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, in the presence of Tris- Glycine buffer) are enhanced in intensity.

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel
• 1.5~2.5 μL per well for general Western blotting transfer

The markers are supplied at a concentration of approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol)

PR1MA™ Tricolor Protein Markers

PR1MA™ Tricolor Protein Markers are three-color protein standards with 10, 12, or 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands, one green and one red band (at 25 kDa and 75 kDa, respectively, in the presence of Tris-Glycine buffer).

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
• 1.5~2.5 μL per well for general Western blotting transfer.

The markers are supplied at a concentration of approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

 
Specifications

Item#	Color	Bands	Range
PR5010-BL	Blue	10	9-180 kDA
PR5011-BL	Blue	11	9-180 kDA
PR5010-TC	Tri-Color	10	9-180 kDA
PR5012-TC	Tri-Color	12	9-245 kDA
PR5013-TC	Tri-Color	13	9-245 kDA

 

PurePlus® PCR Plates

• PurePlus® plates are tested for RNase/DNase lot by lot to ensure pure product
• Our plates are lot certified free of Human DNA contamination
• Our plates are tested for ATP lot by lot to ensure pure consistent product
• Broad product range. Over 18 styles of plates fit most major thermocyclers
• These plates are autoclavable to 122° C for 15 minutes at 15PSI

Ultra Clear and Ultra Clean
We make our PurePlus® Polymerase Chain Reaction (PCR) consumables without additives so you don't need to wonder what is different about the plates, tubes, or strips you are using this week. We also test every batch for contamination by Human DNA as well as the more common tests that other suppliers offer for RNase and DNase enzymes. Many of our plates are printed with black alpha numeric reference grids to make it easier for you to keep track of your samples. We also offer optically clear caps and opaque black or white plates for Real Time PCR. And we also offer re-usable silicone sealing mats and polypropylene sealing film. Labcon PurePlus® PCR tubes, strips and plates are designed to fit many popular thermocyclers including brands like Applied Biosystems, Eppendorf, Thermo Scientific, and Bio Rad. All our PCR disposables are made from 100% virgin polypropylene and are lot tested and certified to be free of RNase, DNase, and Human DNA. Autoclavable. Our pure resin consumables are available in strips, individual tubes, plates in clear natural or colors.

Our PurePlus® sterile disposables, like all our other sterile products, receive valid sterilization processing compliant with ISO 11137. So unlike "pre-sterile" products from other suppliers you can use PurePlus® PCR disposables for diagnostic and regulated procedures.