Hoefer® Air-Cooled Vertical Electrophoresis System

Compact, rugged, and economical units ideal for educational laboratories with multiple users.

• Maximum glass plate size 18 x 16 cm
• Rugged injection molded construction is durable and assembles easily
• Run two gels with the addition of a divider plate
• Built-in casting stand seals leak free
• Choose from a wide variety of accessories
• Plates, clamps, spacers, and combs are interchangeable with the SE600 Series 

Hoefer® Air-Cooled Complete Unit (SE400-15-15)

• Lower Buffer Chamber/Casting Stand on Leveling Base with High Voltage Leads, 1/ea
• Upper Buffer Chamber, 1/ea
• Safety Lid with Electrodes, 1/ea
• Glass Plates, 18 x 16 cm, 2/pk
• Clamp Assemblies, 16 cm, 2/pk
• Cams, 2/pk
• Slotted Gasket for Upper Buffer Chamber, 1/ea
• Bubble Level, 1/ea
• Laminated Gasket for Casting Stand, 1/ea
• Spacer-Mate-Alignment Template, 1/ea
• Wonder Wedge Plate Separation Tool, 1/ea
• Gel Seal, 1/ea
• Comb, 15-well, 1.5 mm thick, 1/ea
• Spacers, 1.5 mm thick, 2 cm wide, 2/pk 

Hoefer® Air-Cooled Basic Unit (SE400)

The same as above without the combs and spacers. Order one SE511 comb and one set of 16 cm long spacers separately.

Specifications
 

Plate Size (W x H)	7.09 x 6.30 In. | 18 x 16 cm
Spacers	0.75 mm, 1.0 mm, 1.5 mm
Max. Power	500 V, 60 mA, 20 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	5.91 x 9.45 x 11.02 In. | 15 x 24 x 28 cm
Weight	6.83 | 3.10 kg

 

Hoefer® Tall Air-Cool Vertical Electrophoresis System

Compact, rugged, and economical units ideal for educational laboratories with multiple users.

• Maximum glass plate size 18 x 24 cm
• Rugged injection molded construction is durable and assembles easily 
• Run two gels with the addition of a divider plate
• Built-in casting stand seals leak free
• Choose from a wide variety of accessories
• Plates, clamps, spacers, and combs are interchangeable with the SE600 Series 

Hoefer® Tall Air-Cool Complete Unit (SE410-15-15)

• Lower Buffer Chamber/Casting Stand on Leveling Base with High Voltage Leads, 1/ea
• Upper Buffer Chamber, 1/ea
• Safety Lid with Electrodes, 1/ea
• Glass Plates, 18 x 24 cm, 2/pk
• Clamp Assemblies, 8 cm, 2/pk
• Clamp Assemblies, 16 cm, 2/pk
• Cams, 2/pk
• Slotted Gasket for Upper Buffer Chamber, 1/ea
• Bubble Level, 1/ea
• Laminated Gasket for Casting Stand, 1/ea
• Spacer-Mate Alignment Template, 1/ea
• Wonder Wedge-Plate Separation Tool, 1/ea
• Gel Seal, 1/ea
• Comb, 15-well, 1.5 mm thick, 1/ea
• Spacers, 1.5 mm thick, 2 cm wide, 2/pk 

Hoefer® Tall Air-Cool Basic Unit (SE410)

The same as above without the combs and spacers. Order one SE511 comb and one set of 24 cm long spacers separately.

Specifications
 

Plate Size (W x H)	7.09 x 9.45 In. | 18 x 24 cm
Spacers	0.75 mm, 1.0 mm, 1.5 mm
Max. Power	500 V, 60 mA, 20 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	5.91 x 9.45 x 14.19 In. | 15 x 24 x 36 cm
Weight	7.94 lb | 3.60 kg

 

Hoefer® Power Supplies

Small footprint, large handle, and simple operation make Hoefer® Power Supplies easy to set up and use in your lab. With a large, easy-to-read LED, simply set the constant parameter, set the timer (if desired), and push start.

• Constant voltage or constant current mode with automatic crossover
• Four sets of 4 mm output jacks
• Continuous runs or a 999-minute timer
• Automatic recovery after power failure
• Open circuit detection
• Short circuit protection

Modes of operation

Constant Voltage Mode: the power supply will attempt to maintain the user selected voltage by automatically adjusting the current flow.

Constant Current Mode: the power supply will attempt to maintain the user selected current flow by automatically adjusting the voltage supplied.

Automatic Crossover: when the constant parameter (voltage or current) cannot be maintained due to power supply operating limits, the power supply automatically switches to maintaining the other parameter.

• PS300B 300 Volt Power Supply is designed for most electrophoresis techniques.
• PS200HC 200 Volt High Current Power Supply is ideal for large electrophoretic blotting applications and for low voltage electrophoresis runs.
• PS600 600 Volt Power Supply is designed for electrophoresis and blotting techniques, including large format and high throughput applications.

Specifications
 

Item #	PS300B	PS200HC	PS600
Voltage	10 to 300 V in 1 V step	5 to 200 V in 1 V step	10 to 600 V in 1 V step
Current	4 to 500 mA in 1 mA steps	10 to 2000 mA in 1 mA steps	4 to 750 mA in 1 mA steps
Max. Wattage	90 W	200 W	150 W
Timer	0 to 999 minutes	0 to 999 minutes	1 to 999 minutes, 1 min increment
Output Modes	Constant voltage or current
Output Jacks	4 pairs in parallel, 4 mm plugs
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	80%	80%	0 to 80%, noncondensing
Voltage Input	100 to 240 VAC, 50/60 Hz
Dims (D x W x H)	11.02 x 4.92 x 13.18 In. | 28 x 12.5 x 33.5 cm
Weight	5.29 lb | 2.4 kg

 

• Uniform Illumination | Four 302 nm Bulbs
• Adjustable UV Safety Cover | Easy Access to Gels
• Large Scratch Resistant Viewing | 16.5 x 13.5 cm

The E3100 Compact UV Transilluminator has a surprisingly small footprint (just over 12 x 8 In.) yet provides industry leading performance. It will accommodate one or two mini gels on the 16.5 x 13.5 cm scratch resistant viewing surface.

The integrated UV blocking cover is made from a polymer that will not fluoresce in UV light. This allows clear viewing of DNA bands through the cover yet offers full UV blocking protection. Two friction hinges on the front edge of the cover allow for full adjustment. Position the cover horizontally for general viewing, tilt it at an angle for gel access and band cutting, or open fully to use the SmartDoc™ Imaging Enclosure (sold separately) for gel imaging with a smartphone.

The transilluminator’s four 6W bulbs evenly illuminate the viewing surface, and the 302 nm wavelength is ideal for Ethidium Bromide, SmartGlow™, SYBR Green®, Gel Green™ and many other common gel stains.  Spacing of the UV bulbs, coupled with a reflector system and special diffusing filter glass provides exceptional uniformity across the viewing surface. This allows for quantification of DNA and higher quality imaging when used with a camera system.

Specifications
 

Light source 4 x 6W UV Light Bulbs	4 x 6W UV Light Bulbs
Safety cover UV blocking polycarbonate	UV blocking polycarbonate
Exterior dimensions	10 x 8 x 2.75 In. | 25.4 x 20.32 x 6.99 cm
Electrical	12VDC (AC power adapter included)
Output wavelength	Peak at 302 nm
Viewing surface	6.5 x 5.3 In. | 16.5 x 13.5 cm
Weight	2 lbs. | 0.9 kg.
Warranty	2 years

Item # E3100 is proudly made in the USA! Click here for more made in America products!
 

Hoefer® Standard Transfer Tank

The TE42 Standard Transfer Tank quickly and evenly transfers proteins and nucleic acids from polyacrylamide or agarose gels onto nylon, nitrocellulose, or PVDF membranes. The four slot design of the TE42 enables either cooled or non-cooled protein or nucleic acid transfers. The optional heat exchange module is easily incorporated into the TE42 enabling transfer of up to 2 gels simultaneously. When used without the optional heat exchange module, up to four gels can be transferred at one time.

• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble cassettes—ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Optional TE47 heat exchanger—provides excellent buffer temperature control when used with an external cooling water bath
• Heat exchanger from an SE600 series electrophoresis unit can be substituted for the TE47 heat exchanger. 

Hoefer® Standard Transfer Tank Includes

• Lower Chamber, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 2/pk
• Foam Sponges, 6 mm thick, 2/pk
• Foam Sponges, 3 mm thick, 4/pk
• Cassette Hook, 1/ea
• Blotter Paper (14.5 x 21.5 cm), 25 sheets/pk

Specifications
 

Capacity	Up to four 15 x 21 cm gels (w/out cooling) Up to two 15 x 21 cm gels (w/ cooling)
Max. Power	100 V, 2 A, 200 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	12.01 x 3.15 x 5.12 In. | 30.5 x 8 x 13 cm

 

Hoefer® Standard Transfer Tank with Cooling Chamber

TE62 Standard Transfer Tank with Cooling Chamber quickly and evenly transfers proteins and nucleic acids from polyacrylamide or agarose gels onto nylon, nitrocellulose, or PVDF membranes while providing excellent temperature control.

The TE62 is designed to accept up to four cassettes simultaneously with cooling. Each cassette holds a 15 x 21 cm gel, or as many as four 7 x 10 cm mini gels.

• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble cassettes - ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Built-in ceramic heat exchanger - provides excellent temperature control when used with an external cooling water bath  

Hoefer® Standard Transfer Tank with Cooling Chamber Includes

• Lower Chamber with Heat Exchanger, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 4/pk
• Foam Sponges, 6 mm thick, 4/pk
• Foam Sponges, 3 mm thick, 8/pk
• Cassette Hook, 1/ea
• Blotter Paper (14.5 x 21.5 cm), 25 sheets/pk 

Specifications
 

Capacity	Up to four 15 x 21 cm gels Up to sixteen 7 x 10 cm gels
Max. Power	100 V, 2 A, 200 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	12.60 x 11.02 x 6.50 In. | 32 x 28 x 16.5 cm

 

Hoefer® Mighty Small Transfer Tank

The TE22 Mighty Small Transfer Tank quickly and efficiently transfers proteins and nucleic acids from small gels to nylon, nitrocellulose or PVDF membranes. Maximum Gel Size 9 x 10 cm.

• Transfer as many as four small gels, up to 9 x 10 cm in less than an hour
• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble and load cassettes—ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Built-in ceramic heat exchanger—provides excellent temperature control when used with an external cooling water bath
• Built-in alumina-covered cooling channel provides excellent temperature control with no more than a 5°C temperature increase during a typical run

Hoefer® Mighty Small Transfer Tank Includes

• Lower Chamber with Heat Exchanger, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 4/pk
• Foam Sponges, 6 mm thick, 4/pk
• Foam Sponges, 3 mm thick, 8/pk
• Cassette Hook, 1/ea
• Blotter Paper (9 x 10.5 cm), 25 sheets/pk 

Specifications
 

Capacity	Up to four 9 x 10 cm gels
Max. Power	100 V, 500 mA, 50 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	6.50 x 5.51 x 9.45 In. | 16.5 x 14 x 24 cm
Weight	4.441 lb | 2.00 kg

 

Gel Crusher™ Spin Column

• Creates uniform gel fragments with no chunks
• Fits in standard microtubes
• Increases DNA yield
• For use in molecular cloning, PCR, restriction digestions, sequencing, and labeling protocols
• Fits 2 mL receiver tubes 

MTC Bio’s Gel Crusher™ Spin Columns are designed to replace labor-intensive gel fragmentation steps (crush+soak method, etc.). Commonly used in molecular cloning, PCR, restriction digestions, sequencing, and labeling protocols, these spin columns can be used to fragment gel slices (typically cut with a gel extractor or razor blade) into smaller pieces.

These smaller uniform pieces will produce greater yields and can be easily pipetted using wide bore tips into an NaCl solution. This reduces the time to create a fine slurry for further elution, filtration, incubation, etc. Place the Gel Crusher™ column in the 2 mL microtube and centrifuge the column/tube assembly in a standard benchtop microcentrifuge for 5 minutes at maximum speed (generally approximately 15,000 x g).

The gel will be forced through the 600 uM holes into the 2 mL receiver tube. The crushed fragments will be small enough to be aspirated/dispensed through a pipette tip.

Reversible Racks

• Alphanumeric markings and are supplied in packs of 5.
• Choose 60 well (5 x 12) or 96 well (8 x 12) formats
• Reversible racks, accept 0.5 and 1.5 to 2.0 mL microtubes
• Alphanumeric marking on each well
• Made of polypropylene   

Azure HRP Stripping Buffer

Works by removing the primary and secondary antibodies from the membrane

Stripping buffer for stripping and re-probing chemiluminescent Western blots.

• Provided as a 1x ready-to-use solution
• Size: 500mL

Azure Aqua Transfer Cell

Perform a quick 1 hour transfer or transfer overnight at a lower voltage

Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.

Includes:

• 2 transfer cassettes
• 6 sponges
• 2 Azure Aqua cooling units
• Tank and upper lid with power cables
• Transfer Electrode Cores

Features

The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.

Chemical reagent compatibility

Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.

 
Cleaning Instructions

Before using, wash all components with a neutral detergent and rinse well with ultra pure water.

Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.

Specifications
 

Azure Transfer Buffer

This proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.

• Provided as a 50x concentrated solution. Sufficient for 50-100 blots
• Size: 500mL

Features

• High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
• Efficient—enhanced transfer efficiency for proteins of all sizes
• Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
• Convenient—use your existing wet transfer electrophoresis apparatus

Azure ChemiWriter ECL Pen

Chemiluminescent pen for blot annotation

The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.

Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.

Quantity: 1 pen

Features

Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.

• Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
• There are two heads to the pen, a fine point tip and a thicker chisel point tip.
• We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
• The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
• For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
• Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
• Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.