PR1MA™ Bst DNA Polymerase

PR1MA™ Bst polymerase (patent pending) is a recombinant, truncated, thermostable Bacillus stearothermophilus DNA polymerase with high reverse transcriptase and strand-displacement activities, ideal for isothermal amplification of RNA and DNA targets. PR1MA™ Bst polymerase has increased sensitivity and speed relative to other Bst polymerases and can incorporate dUTP. 

Use PR1MA™ Bst polymerase to develop LAMP assays with high sensitivity and specificity.

• Lacks 5’ to 3’ exonuclease activity
• Only Bst polymerase in the market with robust RT and DNA polymerase activity
• Thermostable, working temperature range 64 - 72°C
• Tolerant to inhibitors
• Storage temperature: - 20°C

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCls
• 1 mM DTT
• 0.1  mM EDTA
• 0.05% Tween – 20
• 0.05% NP - 40 substitute
• pH = 7.5

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.  
 

2025 LabValues Promotion!
For a limited time enjoy discounted pricing on select
Labnet Enduro™ UV Transilluminators (U1001 and U1002)!

 

See flyer for more details.

 

Special price applies only to the specified item; no substitutions.

Cannot be combined with other discounts or pricing. Not valid on prior purchases.

Promo is valid 01/06/2025 – 06/30/2025 or while supplies last.

All terms and conditions apply.

Enduro™ UV Transilluminators

PR1MA™ Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers are blue protein standards with 10 or 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, in the presence of Tris- Glycine buffer) are enhanced in intensity.

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel
• 1.5~2.5 μL per well for general Western blotting transfer

The markers are supplied at a concentration of approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol)

PR1MA™ Tricolor Protein Markers

PR1MA™ Tricolor Protein Markers are three-color protein standards with 10, 12, or 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands, one green and one red band (at 25 kDa and 75 kDa, respectively, in the presence of Tris-Glycine buffer).

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
• 1.5~2.5 μL per well for general Western blotting transfer.

The markers are supplied at a concentration of approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

 
Specifications

Item#	Color	Bands	Range
PR5010-BL	Blue	10	9-180 kDA
PR5011-BL	Blue	11	9-180 kDA
PR5010-TC	Tri-Color	10	9-180 kDA
PR5012-TC	Tri-Color	12	9-245 kDA
PR5013-TC	Tri-Color	13	9-245 kDA

 

PurePlus® PCR Plates

• PurePlus® plates are tested for RNase/DNase lot by lot to ensure pure product
• Our plates are lot certified free of Human DNA contamination
• Our plates are tested for ATP lot by lot to ensure pure consistent product
• Broad product range. Over 18 styles of plates fit most major thermocyclers
• These plates are autoclavable to 122° C for 15 minutes at 15PSI

Ultra Clear and Ultra Clean
We make our PurePlus® Polymerase Chain Reaction (PCR) consumables without additives so you don't need to wonder what is different about the plates, tubes, or strips you are using this week. We also test every batch for contamination by Human DNA as well as the more common tests that other suppliers offer for RNase and DNase enzymes. Many of our plates are printed with black alpha numeric reference grids to make it easier for you to keep track of your samples. We also offer optically clear caps and opaque black or white plates for Real Time PCR. And we also offer re-usable silicone sealing mats and polypropylene sealing film. Labcon PurePlus® PCR tubes, strips and plates are designed to fit many popular thermocyclers including brands like Applied Biosystems, Eppendorf, Thermo Scientific, and Bio Rad. All our PCR disposables are made from 100% virgin polypropylene and are lot tested and certified to be free of RNase, DNase, and Human DNA. Autoclavable. Our pure resin consumables are available in strips, individual tubes, plates in clear natural or colors.

Our PurePlus® sterile disposables, like all our other sterile products, receive valid sterilization processing compliant with ISO 11137. So unlike "pre-sterile" products from other suppliers you can use PurePlus® PCR disposables for diagnostic and regulated procedures.
 

Plate-Sealing Roller

The plate-sealing roller features a smooth spinning, thermoplastic elastomer sealing surface with a durable plated metal handle.

• Reusable, autoclavable
• Provides a large 4 inch (10.16 cm) wide sealing surface
• A 10 minute or greater dwell me at room temperature before processing increases the film-to-plate bond