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Scientific Lab Products Application Library

Blotting Techniques Enhanced by the Use of Type I Ultrapure Water

Oct7

Blotting Techniques Enhanced by the Use of Type I Ultrapure Water

Northern, Southern and Western blotting techniques depend on the use of Type I ultrapure water to obtain high quality results. Water used for blotting purposes should be nuclease-, endotoxin- and bacteria-free to protect the DNA, RNA and proteins under investigation.

Axygen® 96 Well Half-skirt Microplates for PCR are Comparable with Competitors

Oct12

Axygen® 96 Well Half-skirt Microplates for PCR are Comparable with Competitors

This report presents how the Axygen® 96 well half-skirt PCR microplates performed in terms of consistency, accuracy, and functionality as compared to its market competitors.

Performance of Axygen® Maxymum Recovery® Pipet Tips

Oct5

Performance of Axygen® Maxymum Recovery® Pipet Tips

In this report, Axygen's Maxymum Recovery pipet tips were used and tested for its dependability in sample preparation and its accuracy in sample delivery. Results showed that Axygen's Maxymum Recovery pipet tips retained lesser fluid compared to other tips tested.

Ultrapure Water Free from Biologically Active Impurities Suitable for PCR

Mar31

Ultrapure Water Free from Biologically Active Impurities Suitable for PCR

This report shows a list of contaminants commonly found in water that can affect DNA amplification by PCR. With the help of ELGA's PURELAB Water Purification Systems, ultrapure water recommended for PCR reactions can be achieved.

Isolation of High Purity, High Quality DNA from Buccal Swabs Using the Isohelix Xtreme DNA Kit (Cat. No: XME-5/50)

Mar30

Isolation of High Purity, High Quality DNA from Buccal Swabs Using the Isohelix Xtreme DNA Kit (Cat. No: XME-5/50)

This report shows how the isolation of buccal swab samples using the Isohelix Xtreme DNA Kit combined with the Isohelix Dri-Capsules provides better yields of intact DNA samples for use in a variety of downstream applications.

NanoPhotometer®: Small Volume Spectrophotometric Applications in Biotechnology

Mar29

NanoPhotometer®: Small Volume Spectrophotometric Applications in Biotechnology

Playing an important role before, during, and after nucleic acid (NA) labeling, Implen's NanoPhotometer™ P-Class offers quick determination of NA concentration through spectrophotometric measurements. Requiring only a small sample volume of less than 1µl and allowing recovery of analysed samples, the NanoPhotometer™ P-Class is perfect for quantitative and qualitative NA analyses before and after a Click-Reaction. This report shows how single- and multi-labelling of nucleic acids through Click-Chemistry and with the help of the NanoPhotometer™ P-Class brings in a more efficient daily laboratory process.

Molecular Lipophilicity Evaluation in Drug Design Study

Mar28

Molecular Lipophilicity Evaluation in Drug Design Study

In this application note, results of the conducted comparative study was achieved with the help of Hettich's ROTOFIX 32. The ROTOFIX 32 was used to centrifuge vialed phases for complete phase separation and n-octanol phase eradication of solutions involved.

Ultrapure Water Enables Excellent Chromatographic Performance for LC-MS Analysis

Jan22

Ultrapure Water Enables Excellent Chromatographic Performance for LC-MS Analysis

In this application note, ELGA highlights the importance of using ultrapure water in achieving successful Liquid Chromatography Mass Spectrometry results and analyses. ELGA also presents the types of water impurities that may affect chromatographic performance and mass spectral data collection and provides recommendations on how these possible sample contaminants can be avoided.
Ultrapure Water Essential for the Direct Determination of Bisphenol A by HPLC-MS/MS

Jan21

Ultrapure Water Essential for the Direct Determination of Bisphenol A by HPLC-MS/MS

ELGA tested a selection of their water purification systems to showcase how effective these are in BPA removal below HPLC-MS/MS detection limit. The featured study demonstrates how suitable ELGA PURELAB water purification systems are for use in BPA determination through HPLC-MS/MS.
Pure Water for the Modern Clinical Laboratory

Jan20

Pure Water for the Modern Clinical Laboratory

This ELGA application note provides a better understanding on why research and development laboratories need clinical grade water. Key points presented include the standards for clinical grade water in laboratories, usage of clinical grade water, effects of water impurities, and water purification technologies.
Meeting the Demands of IPC-MS with Type I Ultrapure Water

Jan19

Meeting the Demands of IPC-MS with Type I Ultrapure Water

In this note, ELGA explains the importance of using highly purified water to achieve reliable and reproducible Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) results. Aside from providing a clearer understanding on the impurities that may affect ICP-MS techniques, the process of producing purified water for ICP-MS analyses was presented as well.

Purified Water for Mammalian and Bacterial Cell Culture

Jan11

Purified Water for Mammalian and Bacterial Cell Culture

In this application note, ELGA presented the importance of using purified water in mammalian cell culture research -- how it is the main component of buffers and media used in cell culture studies as well as how it can significantly affect the success of cell culture processes. With Type I ultrapure water as well as Type II purified water, contaminants that might impede culturing processes can be avoided.
A Novel High-throughput Post-PCR DNA Purification System for Improving Lab Productivity

Jan7

A Novel High-throughput Post-PCR DNA Purification System for Improving Lab Productivity

In this application note, Diffinity Genomics showcases the innovatively designed RapidTip®, the pipette tip offering outstanding molecular separation technology for scientific research laboratories. The RapidTip® combined with efficient liquid handling systems effectively purifies DNA from PCR samples at a significantly improved throughput rate without additional costs. Furthermore, it provides excellent yield, rapid sample analysis and results turn-around time as well as reduced environmental and waste impact.
A Low-cost, Automated Post-PCR DNA Purification System for Use in Dispersed Laboratories

Jan6

A Low-cost, Automated Post-PCR DNA Purification System for Use in Dispersed Laboratories

Diffinity Genomics presents an innovative molecular separation technology and functional pipette tip, the RapidTip®, specifically designed for low-cost automated bench top pipetting systems. The RapidTip secures optmized DNA purification from PCR samples and provides quick sample turn-around time, minimized interuption to sample preparation, and lowered waste and environmental impact.

Use of the Diffinity RapidTip® in amplicon library preparations improves sequencing accuracy with Roche 454® Genome Sequencer FLX Systems

Jan5

Use of the Diffinity RapidTip® in amplicon library preparations improves sequencing accuracy with Roche 454® Genome Sequencer FLX Systems

Next-generation sequencing (NGS) systems rely on carefully prepared templates to generate accurate, high throughput data. In the Roche 454 GS FLX system, sequencing templates are prepared by emulsion-based clonal amplification of a DNA library using specialized fusion primers and capture beads. Multiplex Identifiers (MIDs) may be added to the design of the fusion primers and included during library preparation as a barcoding tool to identify specific amplicons. MID-labeled amplicons must be quantified individually and pooled for emPCR (emulsion-based clonal amplification). It is difficult to quantify amplicons if they are not separated from surplus primers in the library preparation step.

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