Isohelix™ DNA/RNA Buccal Swabs

The Isohelix™ range of DNA Buccal Swabs has been specifically designed to give increased yields of high quality buccal cell and genomic DNA. Buccal cell sampling offers a viable alternative to blood collection and, together with the Isohelix™ range of DNA Isolation and stabilization kits, enables high yields of pure, intact DNA to be collected easily and quickly.

Isohelix DNA swabs are suitable for both human and veterinary use and offer significant advantages over other DNA swab designs in terms of efficiency of cell collection by the unique swab matrix, which combined with a quick release surface, maximizes yields of DNA. Swab batches are ethylene oxide treated and routinely tested for human DNA contamination.

• High-yielding swabs for buccal cell DNA collection
• Unique swab matrix greatly improves DNA yields
• Alternative to blood collection
• Suitable for human and veterinary use
• Easy to handle and quick to use
• Available in single and twin sterile packs

Applications

• Research Genotyping Applications
• Veterinary Genotyping and Diagnostics
• Paternity and Heredity Services
• Forensics and DNA Population Studies

Additional for SK-1S:

DNA Buccal Swab Pack: 1 buccal swab with 5 mL tube and cap

Additional for SK-2S:

DNA Buccal Swab Pack: 1 buccal swab with 2ml tube and special release cap

Additional info for SK-3S, SK-4S & SK-5S:

SK-5S: 3 Buccal Swabs per wrapper

Additional info on RapiDri™Pouch

Once placed into the RapiDri™ pouch, the swab is quickly stabilized to maximize the DNA yield without loss of integrity, over many months at ambient temperatures. The entire contents are Ethylene Oxide treated and routinely tested for human DNA contamination. The RapiDri™ pouch also protects the DNA from cross-contamination and conveniently acts as a transport pack, meeting.

GeneFiX™ Saliva DNA/RNA Collection and Isolation

The GeneFix™ range of DNA/RNA products has been designed to simply maximize the yields and purity of DNA/RNA collected and stabilized from Saliva. Isohelix has over the years built a leading reputation for designing and manufacturing DNA/ RNA sampling and purification products, built around their unique High Yielding DNA Buccal Swabs together with their associated kits to stabilize and isolate your DNA/RNA.

GeneFiX™ products use many shared chemistries and knowledge designs from our existing swab DNA isolation kits, and where technically appropriate these have been re-configured, or method developed to optimize their use solely on saliva stabilized either from the GeneFiX™ collectors or other saliva collection devices. The resulting DNA/RNA collected through GeneFiX™, as with the Isohelix Buccal DNA, can be used in the most demanding downstream processes.
 

• Stabilizes DNA & RNA at Room Temperature
• Total DNA Yields Exceeding 300 μg/µL
• Guanidinium Free Pre-Filled Stabilization Buffer
• Optimized with GeneFix Isolation Kits
• Non-Toxic - Non-Hazardous - Easy to Use
• Unique Funnel Design Prevents Spillages or Buffer Flow Back
• Suitable for Field, Home Use & Clinic-Based Sampling
• Collection Tube Specified to 95kPa for Leak Proof Mailing

DNA/RNA Saliva Collectors

GeneFix™ is an easy-to-use and economical DNA/RNA saliva collection device, designed specifically to store and stabilize long-term DNA for 1 or 2 mL saliva samples, and now additionally our new High Capacity collector for 3 mL samples. The collection tube is pre-filled with a fully tested and chemically optimized stabilization buffer. The simple screw-on funnel enables the saliva sample to be delivered directly and quickly into the buffer.

Clear, legible markings on the GeneFiX™ collection tubes indicate the correct volume of Saliva to be delivered, before removing and sealing the tube, the unique funnel design prevents spillages and flow back even when accidentally knocked over. The collection tubes will withstand defined physical pressures that have been specified for leakproof sample transport and are designed for either manual or more automated application with printed bar codes and caps options being suitable for auto de-capping machines.

• GFX-01 Can Collect 1 mL of Saliva & Stabilize DNA For Up To 60 Months
• GFX-02 Can Collect 2 mL of Saliva & Stabilize DNA For Up To 60 Months
• Xtra DNA Collect GFX-03 Can Collect 3 mL of Saliva &stabilize DNA For Up To 60 Months

Isohelix™ Dri-Capsules

Designed to Quickly & Efficiently Dry Buccal Swabs & Preserve DNA.

SGC Dri-Capsules are a Fast, Simple & Easy way of Stabilizing Buccal Swab Samples

• Long-Term Stability at Room Temperature
• Reduces DNA Cross Contamination Risk
• Non-Toxic & Inert Coating – Very Easy to Handle
• Ideal for Remote & Field Sample Collection
• 50 Isohelix™ Dri-Capsules all individually foil wrapped

MADE FOR SK-1S BUCCAL SWABS

The 5 mL Tube in The SK-1S Works Perfectly With Our Dri-Capsules (SGC)

Isohelix™ Xtreme RNA Isolation Kit

Quick & Easy Silica-Based Spin Column Isolation Kit for Saliva & Buccal Samples

XMR is Designed to Maximize Results & is Suitable for Even the Most Demanding Downstream Applications

• High yield & purity RNA
• Enzymatic method for disruption of RNAses
• Scalable to different sample input volumes
• Suitable for Viral RNA extraction
• No toxic phenol, chloroform, β-mercaptoethanol, or guanidine salts
• Ideal for rt-qPCR & other downstream RNA applications

Fully Optimized for Both Stabilized Saliva & Swab Samples
 
 

Isohelix™ BuccalPrep Plus DNA Isolation Kit

• Latest optimized chemistry for the highest purities
• Includes lysis and stabilization buffers
• High purity values as measured by A260/280
• High yields of intact DNA with negligible RNA
• All the same advantages as Buccal-Prep kits

The Buccal-Prep Plus DNA Isolation Kit is our latest precipitation-based chemistry combining all the necessary optimized buffers to fully lyse and simply purify DNA from Buccal swabs. The kit has a fast protocol, producing high yields and purity for all downstream processing applications. As with our other precipitation-based chemistries, no solvents, alcohol, or other external buffers are required, giving fewer processing steps and creating a faster process time.

Isohelix™ BuccalFix Plus DNA Isolation Kit

All the reagents needed to easily and quickly isolate and purify DNA with extremely high yields and purity.

The BuccalFix DNA Isolation Kits are designed to easily and quickly purify DNA already stored in BuccalFix Stabilization buffers. 

The kit comes with all the reagents required to perform the necessary isolation steps, including Proteinase K, to produce DNA with high yields and purity for all downstream processing applications.
The protocol is extremely flexible and easily adjusted if there are varying volumes to be purified. The average processing time for 0.5 mL is well under 1 hour, and no solvents, alcohol, or other buffers are required.  

• Optimized for DNA Buccal Swabs
• Can be scaled up or down for varying volumes
• Gives extremely high yields and purity
• Rapid protocol times
• Can be used in single or automated environments
• Uses optimized chemistries with data history
• Does not co-elute RNA

Isohelix™ Buccalyse Direct To PCR Kit

The Isohelix™ Buccalyse DNA Release Kit produces high DNA yields from buccal swabs (2 to 4 µg) average total yield from an adult) offering significant advantages of ease of handling, speed, and ease of sample preparation with rapid extraction of PCR-ready DNA using a single tube method not requiring centrifugation.

BEK-50 DNA release kit is for 50 samples. BEK-3 is for 3 samples. 

• Quick and easy 1 tube / 1 step kit
• PCR-ready DNA in under 20 mins
• For simple and multiplex PCR
• Ideal for qPCR DNA samples
• Outperforms alternative single-tube kits
• For High Throughput and Robotics
• Developed for DNA buccal swabs

Isohelix™ Xtreme DNA Isolation Kit

The Xtreme DNA Isolation Kit is a silica membrane-based spin column DNA purification kit designed to isolate highly purified, high molecular weight genomic DNA from buccal swabs with minimal losses. The DNA is suitable for use in even the most demanding downstream applications.

The XCU-50 kit is for 50 reactions, while XME-5 is for 5 reactions.

• Very high purity DNA
• DNA free from trace PCR inhibitors
• Optimized for buccal DNA
• Manual or high throughput formats
• No solvent-based chemicals
• Maintains integrity of the genomic DNA
• Improved success in demanding downstream applications

Isohelix™ Buccal-Mag DNA Isolation Kit

High Throughput Magnetic Bead DNA Isolation

BMG is a Fast & Reliable Method for Medium to High Throughput Purification of DNA Samples.

• High Binding Capacity to Maximize Sample Yields
• High Purity & Yield for Enhanced Downstream Performance
• Variable Sample Volumes for Flexible Methodology
• Non-Toxic to Reduce Risk Considerations Over Other Kits
• Makes Use of Original Tubes to Reduce Waste & Cost
• Developed Specifically for Buccal Swabs

Isohelix™ Buccalfix RNA Stabilization & Lysis Kits

Designed to Stabilize RNA Long Term for Buccal Swab Samples.

RFSS Fully Stabilizes RNA & DNA at Room Temperature for up to 4 Weeks

• Stabilizes all RNA Species
• Instant Stabilization
• Room Temperature Storage & Transport
• Compatible with all Isolation Kits (Proteinase K Supplied)
• Includes Flocked Swabs - For Superior RNA Collection

Isohelix™ GeneFix™ Saliva-Prep 2 DNA Isolation Kit

The Isohelix GeneFix™ Saliva-Prep Isolation kit is designed specifically for use with Saliva samples and uses a precipitation method to quickly and easily isolate the DNA up to 2 mL volumes from the GeneFix™ Saliva DNA Collectors.

The GeneFix™ Saliva-Prep kit does not use solvents, columns, or filtration and the reduced number of protocol steps makes it extremely easy to use where a simple yet efficient isolation method is required.

Features

• High yields in excess of 100 µg from 2 mL of Saliva
• High Purity for the most demanding of applications
• Fully optimized for use with Saliva
• Fast handling times, and reduced handling steps
• No Solvents, Columns, or Filtration requirements
• High Throughput Protocol for DeepWell Plates

Isohelix™ Genefix Saliva DNA/RNA Collection - Assisted

• Stabilizes DNA & RNA at Room Temperature
• Guanidinium Free Pre-Filled Stabilization Buffer
• Optimized with GeneFix Isolation Kits
• Includes Absorbant Swabs for Assisted Saliva Collection
• Non-Toxic - Non-Hazardous - Easy to Use
• Unique Funnel Design Prevents Spillages or Buffer Flow Back
• Suitable for Field, Home Use and Clinic-Based Sampling
• Collection Tube Specified to 95kPa for Leak Proof Mailing

GFXA is an easy-to-use assisted DNA saliva collection device that stabilizes DNA long-term

GeneFix™ is a new easy to use and economical RNA saliva collection device, designed specifically to store and stabilize RNA from 1 mL Saliva samples. The collection tube is pre-filled with a fully tested and Non-toxic stabilization buffer. The simple screw-on funnel enables the saliva sample to be delivered directly and quickly into the buffer, and requires no specialist supervision, making remote sampling possible.

Clear, legible markings on the GeneFix™ collection tubes indicate the correct volume of Saliva to be delivered, the design patented funnel prevents spillages and flow back even when knocked over. The collection tubes will withstand defined physical pressures that have been specified to leakproof sample transport.

The tube is compatible with most automated extraction platforms, and the collector is supplied with Proteinase K, to allow RNA isolation using most kits.

GeneFix™ Assisted RNA Saliva Collectors

Assisted collectors are especially useful where donors find it difficult to deliver saliva, such as children. The assisted kits contain the standard GeneFix components and 2 absorbent swabs to assist the saliva collection, the swab is simply squeezed against the inside of the funnel to release the saliva into the 1 mL of stabilization buffer.

SWABS INCLUDED

The GFXA Swabs Are Specifically Designed As A Safe And Non-Invasive Way To Collect Saliva Samples From Individuals That Cannot Provide It Themselves

Isohelix™ Genefix RNA Saliva Collection & Stabilization

Saliva RNA Collection & Stabilization Device for Research Purposes

The GeneFix RFX is an easy-to-use RNA saliva collection device that stabilizes RNA from the moment of collection for 2 months.

• Allows for Isolation of High Yield, High Purity RNA & DNA
• RNA Stabilized at Room Temperature for at least 2 months
• Economic, Easy-to-Use Integrated RNA Stabilization Buffer
• Compatible with Most Manual & HT Isolation Kits
• Unique Funnel Design Prevents Spillages or Buffer Flow Back
• Suitable for Field, Home Use & Clinic-Based Sampling
• Collection Tube Specified to 95kPa for Leak Proof Mailing
• Suitable for COVID-19 Research

Isohelix™ Stoolfix Gut DNA Microbiome Stabilization Kits

The StoolFix Gut Microbiome Collection and Stabilization prefilled tubes use the latest Isohelix™ DNA Stabilization chemistry, fully optimized for fecal microbiome DNA.

To minimize sample handling, the Isohelix™ DNA swabs (SK-3S) are used to collect and transfer the sample into prefilled tubes containing the StoolFix buffer. Once sealed the buffer protects the DNA integrity and maximizes yield for months at room temperature. 

The robust tube is well suited to shipping, combining the collection, transportation, and storage of samples into one tube.

• Combines effectively with Isohelix™ swabs for high yield and versatility
• Transport tested for at-home sampling
• Eliminates the need for cold storage
• Stabilized for months at room temperature
• Minimized sample handling
• Widely compatible chemistry
• Optimal DNA Isolation with BuccalFix Plus DNA Isolation kit (1 x BFP-50 = 25 StoolFix samples)

Isohelix™ DNA/RNA Buccal Mouse Swabs

The MS-001, MS-002, MS-003, and MS-004 Mini DNA Isohelix™ Swabs use the same matrix material as the SK swabs so there is a significant history of use but have reduced dimensions and area. The width of the swab has been reduced from 8 mm to 6 mm. The swabs are Ethylene Oxide sterilized and are available in different swab numbers per wrapper; the break point has been reduced compared to the SK swab allowing it to be used in smaller tubes.

Mini Buccal Swabs are Available in individual packs of 1 & 2 Plus a 1 Swab Pack with 5 mL Tube Option

MS-01, MS-02 & MS-03 Are Easy To Use Buccal Swabs That Have Reduced Dimensions For Smaller Subjects 

• Ambient Storage – No Expensive Storage or Transport
• Easy To Handle & Quick To Use
• Available In Multiple Pack Sizes
• MS-01 Includes a 5ml Tube
• Smaller surface area size
• Very high pro rata DNA yields
• Fits smaller tubes so it can be 96-racked
• Lower break point to fit shorter tubes
• Uses identical swab material to SK series

PERFECT FOR VETERINARY USE

MS Swabs Are Ideally Suited For Veterinary Use Including Small Mammals & Fish

Isohelix™ Proteinase K

• Available in Different Quantities
• Light Reducing Container
• Suitable for -20ºC Storage
• High Quality – Fully QC Tested

Lyophilized proteinase K is ideal for the Genefix™ Saliva Kits. Comes with instructions.

 

Isohelix™ DNA Cleanup Kit

A Secondary Isolation Protocol That Further Improves the DNA Purification from Contaminated Saliva or Buccal Samples

DCU Re-Vitalises & Rescues Failed Samples Due to Poor Sampling or Non-Optimized DNA Isolation Kits

• Re-Claims Failed Purity Samples
• High Purity with 260/280 Ratios to 1.8
• Preserves Yields
• Extremely Rapid Protocol of 30 Minutes
• No Alcohol or Solvents Required
• DNA From Any Source

Isohelix™ BuccalFix DNA Stabilization and Lysis Kits

Stabilizes DNA at room temperature maintaining integrity for downstream process applications.

Isohelix BuccalFix range of stabilizing buffers are designed to completely stabilize the DNA in samples from DNA buccal swabs by inhibiting all enzymatic and microbial activity that occurs following any buccal sampling.

The DNA is fully stabilized and, importantly, the structure and integrity of the DNA is fully maintained for further downstream processing applications.

Once stabilized, the DNA can be stored in the BuccalFix at room temperature for periods in excess of 2 years, and then easily be processed for DNA isolation by the addition of Proteinase K and subsequent isolation steps.

The BuccalFix buffers are offered in bulk, as well as pre-filled into various tube formats to suit both manual and more automated applications where 1D and 2D barcoding and auto de-cap options are valuable. 

• Stabilizes your DNA long-term
• Maintains DNA structural Integrity
• Improves DNA yields and purity
• Room temperature storage & transport
• Accommodates various swab designs
• Compatible with most DNA isolation kits

Isohelix™ Genefix™ Saliva DNA Microbiome Collection & Stabilization

Collection & Stabilization Devices for Saliva Microbiome DNA

MFX Is An Easy To Use Saliva Collection Device That Stabilizes Microbiome DNA For Over 12 Months

• Ambient Storage – No Expensive Storage or Transport
• Instant Stabilization – Provides a Snapshot of the Saliva Microbiome
• No Trace DNA Contamination – Reliable Results
• Compatible to Most Downstream Chemistries
• Non-Toxic – Non-Hazardous – Easy to Use
• Unique Funnel Design Prevents Spillages or Buffer Flow Back
• Suitable for Field, Home Use & Clinic-Based Sampling
• Collection Tube Specified to 95kPa for Leak Proof Mailing

NO FREEZING REQUIRED - Microbial DNA is Perfectly Preserved at Room Temperature

Isohelix™ Spin+Collect™ Devices

Spin+Collect™ is a novel sample recovery device to increase sample yields from swabs and other materials. The Isohelix™ Spin+Collect™ firmly grips the swab or other material whilst exposing maximal surface area for the first step of elution or lysis of the sample. A 1.5 mL microfuge tube is then fitted as a cap and inverted for centrifugation and final release of any sample trapped in the matrix.

• Yields increase over 25%
• Recover samples deeply embedded in the matrix
• Requires a single centrifuge step
• Disposable, sterile, and packaged aseptically
• Tested for Human DNA contamination

Additional info for SC/ST-100

100 x Spin+Collect™ devices packed 5 per bag with 100 x 1.5 mL matched tubes.

Additional info for SC-150

150 x Spin+Collect™ devices packed 5 per bag.

Isohelix™ Genefix™ Salivalyse Direct To PCR Kit

High DNA Yields from Stabilized Saliva Using a Single Tube Method

SEK Offers Significant Advantages of Ease of Handling, Speed & Ease of Sample Preparation

• Quick & Easy 1 Tube / 1 Step Kit
• Fast Preparation Times
• Ideal for qPCR DNA Samples
• Outperforms Alternative Single Tube Kits
• For High Throughput & Robotics
• For Simple & Multiplex PCR

TIME FLIES WITH SALIVALYSE - A must-have for time essential studies.

Isohelix™ Genefix™ Saliva-Mag DNA Isolation Kit

High Throughput Magnetic Bead DNA Isolation

GSM is a Fast & Reliable Method for Medium to High Throughput Purification of DNA Samples.

• High Binding Capacity to Maximize Sample Yields
• High Purity & Yield for Enhanced Downstream Performances
• Variable Sample Volumes for Flexible Methodology
• Non-Toxic to Reduce Risk Considerations Over Other Kits
• Makes Use of Original Tubes to Reduce Waste & Cost
• Developed Specifically for Saliva

These products have been discontinued.

Please contact us for other available options.

Isohelix™ GeneFix™ Saliva Mini and Midi DNA Isolation Kits

The Isohelix™ GeneFix™ DNA kits use either mini or midi-formatted high-performance silica spin columns (specifically optimized) for the purification of DNA from Saliva. The isolated genomic DNA is highly purified with a typical A260/280 ratio above 1.8 and A260/230 above 1.0 with a high molecular DNA weight derived directly from the saliva DNA samples, most importantly showing minimum losses from the easy-to-use protocol.

The Mini format is especially useful when the Scientist wishes to isolate 0.5 mL to 1.0 mL aliquots of saliva stabilized from the GeneFix™ Saliva collector. The midi-format is used when the whole 2 mL of saliva needs to be isolated in a one-batch protocol. The DNA produced from both systems is suitable for use in the most demanding of downstream processing applications.

• High Purity Genomic DNA with Yields in excess of 100 µg
• Fully Optimized for Saliva samples
• DNA free from trace PCR inhibitors
• Designed for both Manual and HT Applications
• No Solvent-based chemicals required
• Ideal for the most demanding downstream Applications

This product has been discontinued.

Please contact us for other available options.

The Isohelix DDK DNA Isolation Kit has been specifically formulated to produce high DNA yield from buccal swabs. The DDK-50 kits are fully optimised for use on buccal samples and offer reduced handling times and increased DNA yields together with many other important technical and practical benefits.

Features

• Includes Stabilisation & Lysis steps
• High yields from buccal swabs
• Optimised to Buccal Cell Swabs
• Fast handling times
• Manual or High Throughput formats
• Considerable test data available
• No solvents, columns or filtration

This is a multiplex PCR kit designed to show the presence and quality of human genomic DNA. The expected results from intact human genomic DNA are bands of 100bp, 200bp, 300bp, 400bp and 600bp together with an internal control band of 500bp derived from Lambda DNA. The presence of the 500bp band in the negative control demonstrates a successful amplification reaction has occurred.