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Promo is valid from 11/04/24 - 12/06/24
All promotions are valid while supplies last. Prices are subject to change without notice.
Not to be combined with any offers or discounts. No institutional pricing. All terms apply.
Use item number +"-VF" or promo code TURKEY24
PR1MA™ TC9639 Gradient Thermal Cycler
The PR1MA™ TC9639 Thermal Cycler provides consistent, reliable results for laboratories with medium to high throughput. From its large, touch-screen user interface to its unique, multi-format block, this cycler is loaded with features. The program wizard and 10 pre-installed programs make it easier than ever to get up and running.
The fully adjustable heated lid prevents condensation and evaporation of samples. Lid temperature can be programmed, and when the block temperature is below 35°C, the lid automatically shuts off. The heating and cooling of the block are precisely and accurately controlled within exceptionally tight tolerances by a proprietary algorithm that simulates sample temperature.
With the multi-format block, there is no need for the expense of multiple interchangeable blocks. The PR1MA™ TC9639 has the flexibility to accept 96 × 0.2 mL tubes, a 96-well plate, 0.2 mL strips or 39 x 0.5 mL tubes all in the same sample block. For protocol optimization, a gradient can be set up across the block. An optional in-situ adapter allows for the incubation of slides. Additionally, the unit may be purchased with a 384-well sample block.
Compatible with PR1MA™ and Accuris™ PCR reagents. For research use only.
A large, full-color touchscreen serves as the operations center where all functions are accessed. From the “Home” screen, simply touch an icon to view the last run program, enter the program library, or use the program wizard to quickly enter a protocol. In addition, icons for favorite programs can be added for faster access.
The program wizard guides users through quickly setting up a protocol on a single screen. Once entered, programs are shown graphically and can be edited by simply touching the step that requires modification. Time and temperature increments can be built into programs as well as slower ramp rates. Storage capabilities are unlimited; programs can be stored in public folders, private (password-protected) folders, or on a flash drive. The full virtual keyboard allows for alphanumeric naming of programs and folders.
Features
- Gradient capabilities- ideal for protocol optimization
- Intuitive touchscreen control- Works even when wearing gloves!
- Upgraded software- Even easier to use than before!
- No need for interchangeable blocks- multi-format accepts:
- 0.2 mL tubes or strips (96)
- 0.5 mL tubes (39)
- 96-well PCR plate
PR1MA™ Taq
- Ideal for routine PCR applications as well as genotyping, colony PCR, and fast PCR
- Improved template affinity and solubility for higher enzyme activity and greater yields
- Proprietary buffer optimized for a variety of assay conditions
- Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer
PR1MA™ Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. PR1MA™ Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.
The polymerase is supplied with a 5X buffer containing MgCl2 and a proprietary mix of enhancers (dNTP's not included). For convenience, PR1MA Taq is also available in a ready-to-use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel. The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.
PR1MA™ LD032 Mini Thermal Cycler
- "Mini" sized, 9 x 7 inch footprint
- Capacity for 32 x 0.2 mL or 4 PCR Strips
- Intuitive touchscreen control
"Mini-sized" doesn't have to mean a sacrifice on features and performance. PR1MA™'s LD032 Mini Thermal Cycler offers advanced programming features, easy-to-use software, and fast, reliable cycling but with a smaller block and lower price point.
Designed for lower throughput needs, the PR1MA™ LD032 Mini Thermal Cycler has a capacity for 32 x 0.2 mL tubes. The wells are arranged in a 4 x 8 format, allowing the block to accept strips of 8 as well as individual tubes. The block heats and cools very quickly - at rates as fast as 5°C/second. A proprietary algorithm controls block temperature, bringing samples quickly to the target temperature without any over- or under-shoot. Excellent block uniformity ensures consistency from sample to sample.
The operation of the thermal cycler is controlled from the large, full-color, touch screen. Icon-driven menus allow users to get running quickly. Programming is extremely simple and includes options for time and temperature increments/decrements, adjustable ramp rates, and auto pause and restart functions. More than 100 typical programs can be stored in memory and easily recalled. After a run, samples can be held at 4°C. An on-screen report is also available at the completion of a run.
The heated lid is self-adjusting - the spring-loaded platen in the lid comes firmly into contact with the tops of the tubes to prevent sample evaporation and condensation. There are no knobs to turn or levers to adjust. The temperature of the heated lid can be changed, or the heating function of the lid may be turned off.
With a footprint less than that of the average laboratory notebook, the PR1MA™ LD032 Mini Thermal Cycler will fit almost anywhere. Its reduced capacity block and economical price make the PR1MA™ LD032 Mini Thermal Cycler the perfect choice for lower throughput laboratories and educational facilities.
Specifications
| Capacity - 0.2 mL tubes | 32 |
| Capacity - 8 x 0.2 mL PCR strips | 4 |
| Temp. Control Range | 0° to 99°C |
| Temp. Control | Simulated sample or block |
| Accuracy/Uniformity | ± 0.25°C | ± 0.25°C |
| Ramp Rate | Heating: 5°C / Sec. (max) Cooling: 5°C / Sec. (max) |
| Lid Temp | 30°C to 112°C, user adjustable |
| Program Memory | Over 100 typical programs |
| Increments/Decrements | Yes, both time and temperature |
| Power Failure Restart | Yes |
| Power Supply | Universal,100 to 240 V, 50 to 60 Hz |
| Dimensions | 7.2 x 9.2 x 6.2 in. | 18.2 x 23.2 x 15.7 cm |
| Weight | 6.4 lbs. | 2.9 kg |
| Warranty | 2 years |
PR1MA™ High Fidelity Polymerase
- 50X higher fidelity than Taq DNA polymerase
- Works with crude DNA sample
- Ideal for cloning, mutagenesis and microarrays
- Produces blunt end products, to clone directly into blunt end vectors
- Optimized buffer system with unique PCR enhancers
For applications requiring highly accurate amplification, choose PR1MA™ High Fidelity DNA polymerase. Modified for better solubility and higher activity across a broad range of ionic conditions, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples.
A 3'-5' proofreading exonuclease activity and an error rate of 4.55×10-7 makes PR1MA™ High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets.
Specifications
| Item | Description | Volume |
| PR1000-HF-S | PR1MA™ High Fidelity DNA Polymerase | 20 Units (Sample) |
| PR1000-HF-200 | PR1MA™ High Fidelity DNA Polymerase | 200 Units (2 U/p1) |
| PR1000-HF-500 | PR1MA™ High Fidelity DNA Polymerase | 500 Units (2 U/p1) |
| PR1000-HF-1000 | PR1MA™ High Fidelity DNA Polymerase | 1,000 Units (2 U/p1) |
PR1MA™ Bst DNA Polymerase
PR1MA™ Bst polymerase (patent pending) is a recombinant, truncated, thermostable Bacillus stearothermophilus DNA polymerase with high reverse transcriptase and strand-displacement activities, ideal for isothermal amplification of RNA and DNA targets. PR1MA™ Bst polymerase has increased sensitivity and speed relative to other Bst polymerases and can incorporate dUTP.
Use PR1MA™ Bst polymerase to develop LAMP assays with high sensitivity and specificity.
- Lacks 5’ to 3’ exonuclease activity
- Only Bst polymerase in the market with robust RT and DNA polymerase activity
- Thermostable, working temperature range 64 - 72°C
- Tolerant to inhibitors
- Storage temperature: - 20°C
Buffer composition
- 50% glycerol
- 50 mM Tris-HCl
- 50 mM KCls
- 1 mM DTT
- 0.1 mM EDTA
- 0.05% Tween – 20
- 0.05% NP - 40 substitute
- pH = 7.5
Important note: Please be sure to use the buffer provided with this product to ensure optimal results.
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
PR1MA™ Taq DNA Polymerase
Saves time and cost by enabling direct PCR amplification of unpurified templates. PR1MA™ Taq DNA polymerase is a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays. It is supplied with 4 M betaine to improve amplification of GC-rich DNA and 30% sucrose to improve amplification from inhibitor-rich substrates such as blood.
- Lacks exonuclease activity
- Thermotolerant up to 98°C
- Resistant to inhibitors, e.g., whole blood
- Ideal for GC-rich templates
- Storage temperature: -20°C
- 10X PR1MA™ buffer, 4 M Betaine, and 30% Sucrose included
Buffer composition
- 50% glycerol
- 50 mM Tris-HCl
- 50 mM KCl
- 1 mM DTT
- 0.1 mM EDTA
- 0.5% Tween-20
- 0.5% NP-40 substitute
- pH = 7.5
Important note: Please be sure to use the buffer provided with this product to ensure optimal results.
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
PR1MA™ Taq DNA Polymerase 1X Master Mix
PR1MA™ DNA polymerase 1X Master Mix saves time and cost by enabling direct PCR amplification of unpurified templates. It contains a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays and is provided as a complete reaction master mix consisting of reaction buffer, dNTPs, MgCl2, and loading dye and only requires the addition of primers and DNA template. Once PCR is complete, the reaction products can be loaded directly into an agarose gel for analysis.
- Lacks exonuclease activity
- Thermotolerant up to 98°C
- Inhibitor Resistant
- Ideal for colony PCR, genotyping, and GC-rich templates
- Storage temperature: -20°C
Important note: Please be sure to use the buffer provided with this product to ensure optimal results.
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
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