Home  >  Tissue/Cell Culture  >  TPP Plastics  >  TPP Tubespin Bioreators  > TPP TubeSpin® Bioreactors

TPP TubeSpin® Bioreactors

Item #: ASTPTBSPINS 

Order # Description Working Vol (mL) Dimensions (mm) Material Max g Force Packaging Quantity
TP87017-200 15 mL Tubespin Bioreactors, Round 1-10 mL 17.1 x 120 PP 15500 40/pk, 5 pks/cs 200/cs
TP87017-400 15 mL Tubespin Bioreactors, Round 1-10 mL 17.1 x 120 PP 15500 40/pk, 10 pks,cs 400/cs
TP87017 15 mL Tubespin Bioreactors, Round 1-10 mL 17.1 x 120  PP 15500 40/pk, 20 pks/cs 800/cs
TP87015 15 mL Tubespin Bioreactors, Conical 1-10 mL 17.1 x 120 PP 15500 40/pk, 20 pks/cs 800/cs
TP87050-60 50 mL Tubespin Bioreactors, Conical 1-35 mL 30 x 115 PP 15500 20/pk, 3 pks/cs 60/cs
TP87050 50 mL Tubespin Bioreactors, Conical 1-35 mL 30 x 115 PP 15500 20/pk, 9 pks,cs 180/cs
TP87056 50 mL Tubespin Bioreactors, Round 1-35 mL 30 x 115 PP 15500 20/pk, 9 pks,cs 180/cs
TP86050 50 mL Tubespin Bioreactors, Conical with Septum 1-35 mL 30 x 115 PP/PE 15500 20/pk, 9 pks/cs 180/cs
TP87450 450 mL Tubespin Bioreactors, Conical 300 mL 96 x 145 PP 3500 32/pk 32/cs
TP87600-3 600 mL Tubespin Bioreactors, Conical 400 mL 100 x 183 PP 3500 3/pk 3/cs
TP87600-13 600 mL Tubespin Bioreactors, Conical 400 mL 100 x 183 PP 3500 13/pk 13/cs
TP87600 600 mL Tubespin Bioreactors, Conical 400 mL 100 x 183 PP 3500 26/pk 26/cs
TP99013 Rack for Three 600 mL Bottles 3 x TP87600 366 x 146 x 70 PP N/A 1/pk, 6 pks/cs 6/cs
TP80617 Rack for 15 / 50 mL Tubes 18 x 15 mL / 10 x 50 mL 209 x 98 x 62 PP N/A 1/ea 1/ea
TP87411 Adapter Plate 32 x 450 mL for Kuhner Shaker 32 x 450 mL 800 x 420 Alu N/A 1/ea 1/cs
TP87431 Adapter Plate 32 x 450 mL for Infors Multitron Shaker 32 x 450 mL 850 x 470 Alu N/A 1/ea 1/cs
TP87611 Adapter Plate 32 x 600 mL for Kuhner Shaker 32 x 600 mL 800 x 420 Alu N/A 1/ea 1/cs
TP87612 Adapter Plate 20 x 600 mL for Kuhner Shaker 20 x 600 mL 500 x 420 Alu N/A 1/ea 1/cs
TP87613 Adapter Plate 16 x 600 mL for Kuhner Shaker 16 x 600 mL 420 x 420 Alu N/A 1/ea 1/cs
TP87631 Adapter Plate 32 x 600 mL for Infors Multitron Shaker 32 x 600 mL 850 x 470 Alu N/A 1/ea 1/cs
TP87633 Adapter Plate 16 x 600 mL for Infors Multitron Shaker 16 x 600 mL 480 x 420 Alu N/A 1/ea 1/cs

TPP's Packed Cell Vol (PCV) tube enables a quick and reproducible method of cell counting based on cell Vol. A quick centrifuge of the cell suspension pipetted into a PCV tube reveals a packed cell pellet in the PCV capillary, which can be easily read with the aid of an Easy read ruler. This non-subjective method is superior to old-fashioned and labor intensive hemacytometer based counting.

Functional Features:

  • Cell suspension sample can be directly quantitated without dilution
  • Multiple samples can be assayed simultaneously
    • Load and spin as many PCV tubes as your centrifuge rotor holds
  • Quick and reproducible
    • Cells can be spun down in 1 minute at 2500xg

Ergonomic Features:

  • Reproducible measurements
    • Graduated capillary model offers immediate quantitation
    • For finer quantitation: The Easy Read Ruler  is used with non-graduated PCV tubes
  • Eliminates expensive automated counters / fragile slides
    • Avoids subjective microscope counting

Quality Assurance:

  • Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
  • Individually wrapped in peel-off plastic wrapper

Protocol Example:

  • Uniformly mix cell suspension collected from TPP  flask/dish/bioreactor
  • Pipet 1000µL of cell suspension into a PCV tube
  • Spin multiple samples for 1 minute at 2500xg in a standard microcentrifuge
  • Record pellet Vol and compare to pre-generated standard curve of cell numbers to packed cell Vol.
    • Standard curve needs to be set up only once per cell line
    • Pipet pre-determined cell suspension Vols (dilutions) into a hemacytometer
    • Calculate and record the number of live cells for each concentration
    • Pipet the identical Vols into the PCV tube, centrifuge and record the numeric in the capillary that corresponds to pellet Vol
    • Plot the hemacytometer readings vs. the PCV readings for each Vol
    • This Standard Curve doesn't need to be redone for the same cell line and moving forward the cell suspension harvested from TPP flasks/dishes/plates only need to be centrifuged in PCV tubes and assayed for cell pellet measurements

High density of cells

    • With a very high density of the cells, the samples must not be diluted. You might simply reduce the sample Vol. Samples from the same series should be treated accordingly. 
    • For bacteria measurement the parameters have to be adjusted, for example higher g-force, smaller Vol, etc.
    • Micro centrifuges with swing-out rotors are preferable.

     

    Order#

    Description

    Quantity

    TP87005

    PCV Cell Counting Tubes (with capillary graduations only)

    50/pk

    TP87007

    PCV Cell Counting Tubes (no graduations)

    50/pk

    TP87008

    TPP Cap for PCV Tubes

    50/pk

    TP87010

    Easy Read Ruler

    1

    Please call us for sample!
PR1MA CoolCube
 
 
Premium Cell Strainers
 
 
TPP Tissue culture flasks
 
 
SKU Number Size Bottom Qty Price Quantity Add to Cart
TP87017-200 15 mL Round 40/pk, 5 pks/cs
475.00
Each
TP87017-400 15 mL Round 40/pk, 10 pks/cs
955.00
Each
TP87017 15 mL Round 40/pk, 20 pks/cs
1904.00
Each
TP87015 15 mL Conical 40/pk, 20 pks/cs
1271.00
Each
TP87050-60 50 mL Conical 20/pk, 3 pks/cs
204.00
Each
TP87050 50 mL Conical 20/pk, 9 pks/cs
554.00
Each
TP87056 50 mL Round 20/pk, 9 pks/cs
863.00
Each
TP86050 50 mL with septum Conical 180/cs
3182.00
Each
TP186550 50 mL with septum, no barcode Conical, Indiv. Wrpd, Sterile 220/cs
1935.00
Each
TP87450 400 mL Conical, Indiv. Wrpd, Sterile 32/pk
736.00
Each
TP87600-3 600 mL Conical, Indiv. Wrpd, Sterile 3/pk
135.00
Each
TP87600-13 600 mL Conical, Indiv. Wrpd, Sterile 13/pk
580.00
Each
TP87600 600 mL Conical, Indiv. Wrpd, Sterile 26/pk
1161.00
Each
TP99013 3 x 600 mL Bottles Rack 6/pk
360.00
Each
TP80617 18 x 15mL or 10 x 50mL Rack 1/ea
12.00
Each
TP87414 Adapter Plate 162 x 50 mL for Kuhner Shaker 1/ea
3964.00
Each
TP87413 Adapter Plate 16 x 450 mL for Kuhner Shaker 1/ea
2495.00
Each
TP87412 Adapter Plate 20 x 450 mL for Kuhner Shaker 1/ea
2879.00
Each
TP87411 Adapter Plate 32 x 450 mL for Kuhner Shaker 1/ea
5561.00
Each
TP87613 Adapter Plate 16 x 600 mL for Kuhner Shaker 1/ea
4509.00
Each
TP87612 Adapter Plate 20 x 600 mL for Kuhner Shaker 1/ea
5199.00
Each
TP87611 Adapter Plate 32 x 600 mL for Kuhner Shaker 1/ea
6585.00
Each
TP87433 Adapter Plate 16 x 450 mL For Infors Minitron Shaker 1/ea
4568.00
Each
TP87633 Adapter Plate 16 x 600 mL For Infors Minitron Shaker 1/ea
4568.00
Each
TP87431 Adapter Plate 32 x 450 mL For Infors Multitron Shaker 1/ea
4692.00
Each
TP87631 Adapter Plate 32 x 600 mL For Infors Multitron Shaker 1/ea
6544.00
Each
TP87355 Solid Screw Cap For 600 mL Bioreactor 36/cs
194.00
Each
TP87356 Filter Screw Cap For 450/600 mL Bioreactor 36/cs
282.00
Each
TPP Bioreactors
TPP TubeSpin® Bioreactors
Special Order
Quantity: