IBI High-Speed Mini Plasmid Kit

The High-Speed Mini Plasmid Kit isolates plasmid DNA from 1-5ml cultures using alkaline lysis and RNase treatment, ensuring minimal genomic DNA/RNA contamination.

Additional Details

The High-Speed Mini Plasmid Kits by IBI Scientific are designed to process a sample size of 1-4 mL of bacterial culture, yielding 20-30 µg for high-copy plasmids and 3-10 µg for low-copy plasmids. With an operation time of 30 minutes or less and a binding capacity of up to 30 µg, these kits offer a rapid and efficient solution for plasmid DNA extraction. Check out our basic guide for plasmid kits for more information.

Technical Details

In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to the glass-fiber matrix in the spin column. The contaminants are washed off with an ethanol-based wash buffer. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol extraction or alcohol plasmid. Typical yields are 20-30 µg for high-copy numbered plasmids or 3-10 µg for low-copy numbered plasmids. The purified plasmid DNA is ready to use for restriction enzyme digestion, ligation, PCR, or sequencing reactions. The entire procedure can be completed in under 30 minutes.

Quality Control

The quality of the High-Speed Plasmid Mini Kit is tested on a lot-to-lot basis, by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid p Bluescript (A600>2U/mL). Following the purification process, a yield of more than 20 µg is expected and the ratio of A260/A280 is between 1.7-1.9. The purified plasmid (1 µg) is used in EcoRI digestion and checked by electrophoresis.

Physical Specifications