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PREMIUM HS-Taq DNA Polymerase Master Mix

Item #: ASPCRREAG8 
Bullseye Taq DNA Polymera

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HS Taq Polymerase, 2X Mix

with HS Buffer I

 

Cat.No.

Size Reactions

HS Taq, 2X Mix (Buffer I)

Final MgCl2

Conc.

BE230301

100

2X HS Buffer I Mix

1.5mM

BE230303

500

2X HS Buffer I Mix

1.5mM

BE230304

1,000

2X HS Buffer I Mix

1.5mM

BE230306

2,500

2X HS Buffer I Mix

1.5mM

Store at -20°CFor in-vitro laboratory use only

 

General Description

Bullseye HS Taq Polymerase, 2X Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.

Bullseye HS Taq Polymerase Mix, the NH4+ buffer system, dNTPs and magnesium chloride are present in HS Taq Pol Mix with HS Buffer I. Each reaction requires 25 µL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 µL.

Bullseye" HS Taq Polymerase Mix is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.

Bullseye" HS Taq Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.

Composition of HS Taq Pol, 2x Mix

Tris-HCl pH 8.5, (NH4)2S04, 3.0mM MgCl2, 0.2% Tween 20Ã, 0.4 mM dNTPs, 0.2 units/µL HS Hot Start DNA Polymerase

Stabilizer

Suggested Protocol using HS Taq Pol, 2x Mix

This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.

  • Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis.
  • The table below shows the reaction set up for a final volume of 50 mL.
  • Important: Mix the solutions completely before use to avoid localized concentrations of salts.

    1. Set up each reaction as follows:

Component

Vol./Reaction

Final Conc.

HS Hot Start Master Mix

with HS Bufffer I

25 µL

1X

Primer A

Variable

0.11.0 µM

Primer B

Variable

0.11.0 µM

Distilled Water

Variable

- - - -

Template DNA

Variable

Variable

TOTAL volume

50 µL

- - - -

2. Mix gently by pipetting the solution up and down a few times.

3. Program the thermal cycler according to the manufacturer's instructions.

4. Each program must start with an initial heat activation step at 95°C for 15 minutes.

For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

A typical thermal cycling program is shown below:

95°C for 15 min. Activate HS Hot Start Polymerase

30-40 cycles:

95°C 30 sec Denature template

45-65°C 30 sec Anneal primer

72°C 1-5 min Elongation

72°C for 5 min Elongation

5. Place the tubes in the thermal cycler and start the reaction.

PNPCRREAG

Magnesium
Mg
Dye
No Dye
Enzymes
Premium
Pkg
Indiv
Type
Hot-Start
SKU Number Vol Magnesium Dye Enzymes Pkg Type Price Quantity Add to Cart
BE230303 500 rxn Mg No Dye Premium Indiv Hot-Start
485.40
Each
  Item is no longer available.  
BE230304 1000 rxn Mg No Dye Premium Indiv Hot-Start
879.84
Each
  Item is no longer available.  
Bullseye Taq DNA Polymera
PREMIUM HS-Taq DNA Polymerase Master Mix
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