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High Throughput Gel Electrophoresis System

25 x 25cm
Item #: ASPCRELCTP4 

Efficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction
Format:
Spin columns
Operation:
Centrifuge/Vacuum Manifold
Binding Capacity:
10µg DNA
Expectant Yield:
80-90% for gel extraction
90-95% for PCR clean-up
Operational Time: 15min. for PCR clean-up/20min. for gel extraction
Applications:
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations

PNRNADNA

Isolation type
DNA
Source Cell
Gel
SKU Number Type Size Teeth Thickness Qty Price Quantity Add to Cart
IB57000 Electrophoresis system 25 x 25cm N/A N/A 1
994.79
Each
IB57020 Casting Tray N/A N/A N/A 1
140.83
Each
IB57030 Casting Fixture N/A N/A N/A 1
133.24
Each
IB57040 Casting Tray N/A N/A N/A 1
231.68
Each
IB57125 Comb N/A 25 1mm 2
148.41
Each
IB57150 Comb N/A 50 1mm 2
148.41
Each
IB57200 Replacement Tank N/A N/A N/A 1
340.73
Each
IB57250 Comb N/A 50 2mm 2
148.41
Each
IB57300 Replacement Lid N/A N/A N/A 1
172.64
Each
IB57305 Replacement Electrode Assembly N/A N/A N/A 1
130.22
Each
IB57325 Comb N/A 25 3mm 2
148.41
Each
IB57350 Comb N/A 50 3mm 2
148.41
Each
IB57425 Comb N/A 25 1.5mm 2
148.41
Each
IB57450 Comb N/A 50 1.5mm 2
148.41
Each
HR-2525 Horizontal
High Throughput Gel Electrophoresis System
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