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RWD Cell Separation Assembly Kit
Applications include immunology research, oncology research, neuroscience, stem cell research, and cell therapy.
RWD magnetic microbead cell separation products consist of Nano-Bead Cell Separation Platform and Nano-Beads (Column Based and Column free). Nano-sized magnetic beads do not need elution, and the separated cells can be directly used in downstream experiments such as flow cytometry, cell culture, single-cell sequencing, and more.
- High purity, high viability, and high recovery rate
- Obtained cells are compatible with downstream applications
- Mild, non-toxic, biodegradable
Kit Includes
- 1 box of LarSep Columns (25/pk)
- 1 LSC Separator (single channel)
- 1 Separation Stand
Work Flow
- Antibody incubation: Incubate the antibody with cell suspension at 4ºC for 10-15 mins.
- Microbead incubation: Incubate the magnetic microbeads with cell suspension at 4ºC for 10-15 mins.
- Magnetic separation: Put the separation column into the magnetic field and add the cell suspension into the column.
- Only cells labeled with magnetic beads will be captured in the column.
- Elution: Remove the column from the magnetic field and add a buffer to elute the positive cells.
Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.
For more information or alternative product recommendations, please contact us.
RWD Human Cell Separation Kits
CD3+ Cell Separation Kit - K1201-10
For positive selection or depletion of CD3+ T cells from peripheral blood, thymus, lymphoid, and tumor tissue.
CD4+ Cell Separation Kit - K1202-10
For positive selection or depletion of T helper cells (Th) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.
CD8+ Cell Separation Kit - K1203-10
For positive selection or depletion of cytotoxic T cells (Tc) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.
CD14+ Cell Separation Kit - K1204-10
For positive selection or depletion of monocytes from human peripheral blood, umbilical cord blood, spleen, lymph nodes, and other samples.
CD45+ Cell Separation Kit - K1205-10
For positive selection or depletion of white blood cells from human peripheral blood and lymphoid tissue samples.
CD19+ Cell Separation Kit - K1206-10
For positive selection or depletion of B cells from human peripheral blood, lymph nodes, bone marrow, and other single-cell suspensions.
Human NK Cell Isolation Kit - K1207-10
For negative selection or depletion of NK cells from single cell suspension of human peripheral blood. Non-NK cells, i.e., T cells, B cells, stem cells, dendritic cells, monocytes, granulocytes, and red blood cells are labeled by biotin antibodies coupled to magnetic beads. High purity NK cells are obtained by isolation of magnetic bead labeled cells.
*All kits are for research use only.
Specifications
Item # | Cell Type | Size | Capacity |
K1201-10 | T Cells | 2 x 1 mL | For 1 x 10^9 Total Cells |
K1202-10 | T Helper Cells | 2 x 1 mL | |
K1203-10 | Cytotoxic T Cells | 2 x 1 mL | |
K1204-10 | Monocytes | 2 x 1 mL | |
K1205-10 | Leukocytes | 2 x 1 mL | |
K1206-10 | B Cells | 2 x 1 mL | |
K1207-10 | NK Cells | 2 x 2 mL |
Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.
For more information or alternative product recommendations, please contact us.
RWD High Activity Enzymatic Digestion Kits
Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.
For more information or alternative product recommendations, please contact us.
Optimized enzymatic hydrolysis formulation for efficient and gentle digestion. Preserves the integrity of surface antigenic epitopes. Store in refrigerators at 4°C or -20°C.
- Targeted optimization of enzymatic hydrolysis formula can ensure high cell viability.
- Preserving cell surface epitopes leads to no effect on downstream experiments.
Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.
For more information or alternative product recommendations, please contact us.
RWD Mouse and Microglia Cell Separation Kits
Mouse CD3+ Cell Separation Kit - K1301-10
For positive selection or depletion of mature T lymphocytes from mouse lymph nodes, thymus, tumors, and other samples.
Mouse CD4+ Cell Separation Kit - K1302-10
For positive selection or depletion of T helper cells (Th) from mouse lymph nodes, thymus, spleen, skin, and other samples.
Mouse CD8+ Cell Separation Kit - K1303-10
For positive selection or depletion of cytotoxic T cells (Tc) from mouse thymus, lymph nodes, spleen, and other samples.
Mouse CD45+ Cell Separation Kit - K1304-10
For positive selection or depletion of white blood cells from mouse lymphoid tissue or non-lymphoid tissue.
Mouse CD19+ Cell Separation Kit - K1305-10
For positive selection or depletion of B cells from mouse spleen and other samples.
CD11b+ (Microglia) cell separation Kit - K1306-10
For positive selection or depletion of microglia from human, adult mice, or rats brain tissue.
Specifications
Item # | Cell Type | Size | Capacity |
K1301-10 | T Lymphocytes | 2 x 1 mL | For 1*10^9 Total Cells |
K1302-10 | T Helper Cells | For 1*10^9 Total Cells | |
K1303-10 | Cytotoxic T Cells | For 1*10^9 Total Cells | |
K1304-10 | Leukocytes | For 1*10^9 Total Cells | |
K1305-20 | B Cells | For 2*10^9 Total Cells | |
K1306-10 | Myeloid Cells | For 1*10^9 Total Cells |
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® Angiogenesis Assay HC Kit
Tunable hydrogel system for 2D gel coating and 3D culture of
angiogenesis tube formation, invasion, and animal injection.
- Hydrogel Kit Only: VitroGel® AAK-HC hydrogel ( 1 mL), AAK Dilution Solution (10 mL)
- Type 1 Full Kit (Hydrogel+Supplements): VitroGel® AAK-HC (1 mL), AAK Dilution Solution (10 mL), AAK Supplement 1 (3 x 500 µL), AAK Supplement 2 (3 x 500 µL)
- Type 2 Full Kit (Hydrogel+Supplements): VitroGel® AAK-HC (1 mL), AAK Dilution Solution (10 mL), AAK Supplement 1 (6 x 500 µL)
- Type 3 Full Kit (Hydrogel+Supplements): VitroGel® AAK-HC (1 mL), AAK Dilution Solution (10 mL), AAK Supplement 2 (6 x 500 µL)
VitroGel® Angiogenesis Assay HC Kit
- Xeno-free functional hydrogel system supporting angiogenesis process
- Control and study the effect of the hydrogel mechanical properties on the angiogenesis process
- Multiple applications in one kit: Tube formation, invasion, and animal injection
- Control the hydrogel properties: Add your own growth factors and compare with positive control
- Harvest cells within 20 minutes at 37 °C without enzyme solution
VitroGel® Angiogenesis Assay Kit is a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesis process. The kit can be used to study the angiogenesis tube formation and invasion on both 2D hydrogel coating and 3D cell culture methods. The VitroGel® system is also good for animal injection for in vivo studies.
Angiogenesis is a highly regulated process that involves the growth of new blood vessels from the existing vasculature. This process plays an important role in both normal developmental processes and numerous pathologies, including wound healing, tumor growth, and metastasis to inflammation and ocular disease.
Traditional angiogenesis assay highly relies on natural extracellular matrix (natural ECM), which has non-adjustable hydrogel compositions and properties. Therefore, our understanding of the angiogenesis process is limited by studying the molecular cues such as growth factors and inhibitors in culture medium only. There is a lack of knowledge on how the properties of hydrogel affect the angiogenesis process.
There are two versions of VitroGel® Angiogenesis Assay Kits:
- VitroGel® Angiogenesis Assay Kit (Cat No. VHM06-K): Ready-to-use with a fixed hydrogel mechanical strength to support the angiogenesis assay with adjustable supplements.
- VitroGel® Angiogenesis Assay HC Kit (Cat No. TWG011-K): Assay kit with a tunable high concentration hydrogel to allow full control of the hydrogel’s mechanical strength with adjustable supplements.
Depending on the kit type, the tunable VitroGel Angiogenesis
Assay HC Kit can contain the following components:
- VitroGel® AAK-HC, a tunable, xeno-free high concentration hydrogel.
- AAK Dilution Solution, for adjusting the hydrogel concentration
- AAK Supplement 1, a hydrogel supplement without vascular endothelial growth factors (VEGFs) for promoting cell attachment and growth.
- AAK Supplement 2, a hydrogel supplement with VEGFs to promote tube formation and as a positive control.
- All the components can be purchased separately.
Besides molecular cues, the VitroGel® Angiogenesis Assay HC
Kit allows researchers to explore the effects of hydrogel mechanical properties
on angiogenesis. The high concentration
VitroGel® AAK-HC hydrogel is room temperature stable and can be adjusted by simply
mixing the hydrogel solution and dilution solution at different rations
(recommend 1:1 to 1:5 v/v) to achieve different mechanical strengths. The
diluted hydrogel solution can be directly mixed with supplement at 2:1 (v/v)
ratio for hydrogel formation. Researchers can adjust the molecular cues of the
hydrogel by adding the growth actors/inhibitors directly to the supplement
before mixing with VitroGel® AAK-HC.
Cells cultured in this system can be further harvested easily with the
VitroGel® Cell Recovery Solution.
TYPE
1 Kit Contents |
VitroGel®
AAK-HC (1 mL) |
AAK
Dilution Solution (10 mL) |
|
AAK
Supplement 1 (3 x 500 µL) |
|
AAK
Supplement 2 (3 x 500 µL) |
|
TYPE
2 Kit Contents |
VitroGel®
AAK-HC (1 mL) |
AAK Dilution
Solution (10 mL) |
|
AAK
Supplement 1 (6 x 500 µL) |
|
TYPE
3 Kit Contents |
VitroGel®
AAK-HC (1 mL) |
AAK
Dilution Solution (10 mL) |
|
AAK
Supplement 2 (6 x 500 µL) |
|
Formulation |
Tunable,
xeno-free functional hydrogel |
AAK Supplement
1: Without VEGFs |
|
AAK
Supplement 2: With VEGFs |
|
Use |
Angiogenesis
Assay, tube formation, invasion, animal injection |
Biocompatibility |
Biocompatible,
safe for animal studies |
Injection |
Injectable
hydrogel for in vivo studies and lab automation |
Cell
Harvesting |
Easy
cell recovery using VitroGel® Cell Recovery Solution |
pH |
Neutral |
Shipping |
Supplements
require dry ice shipment. |
Storage |
VitroGel®
AAK-HC hydrogel: store at 2-8°C |
AAK
Supplement 1 and AAK Supplement 2: store at -20°C |
|
Number
of Uses |
30-180
tests per kit |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
Cyto3D™ Live-Dead Assay Kit
Live dead cell viability analysis for 3D and 2D cell
culture.
The Cyto3D™ Live-Dead Assay Kit is used to determine the live/dead nucleated cells by using a fast one-step staining procedure for analysis on a dual-fluorescence system. This kit is recommended for viability analysis of cells cultured in 3D, 2D coating and on monolayer.
- Ready-to-use
- Fast
- Sensitive
- Excellent for 3D cell cultures
- Cost-effective
Acridine orange (AO) and propidium iodide (PI), both nuclear staining (nucleic acid binding) dyes, are used in this kit. AO is permeable to both live and dead cells and stains all nucleated cells to generate green fluorescence. PI only penetrates the membranes of nucleated cells with compromised membranes and stains the dead cells to generate red fluorescence. Due to the quenching, when cells are stained with both AO and PI, all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red (the PI reduces the fluorescence intensity of the AO by fluorescence resonance energy transfer (FRET)). Non-nucleated materials such as red blood cells, platelets and debris do not fluorescence and are ignored by fluorescence microscopes.
Dual-Fluorescence Viability, using AO and PI, is the
recommended viability analysis method for cell lines, primary cells, and stem
cells.
Specifications
Formulation |
Premixed
acridine orange (AO) and propidium iodide (PI), nuclear staining dyes |
Use |
Live
dead cell viability analysis for 3D and 2D cell culture |
Detection
Method |
Fluorescent |
Excitation/Emission: |
AO
(494/517nm), |
PI
(535/617nm) |
|
Standard
filters |
AO
(GFP), PI (Texas Red) |
For
use with (Equipment): |
Fluorescence
microscope, flow cytometer, microplate reader, fluorescence cell counter. |
Storage |
2 to
8°C (Protect from light) |
Shipping
Conditions: |
Ships
at ambient temperature |
Sizes |
1 mL |
Number
of reactions |
500
(at 2 µL per 100 µL) |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® RGD High Concentration
RGD modified – tunable, xeno-free hydrogel – high concentration (3 mL kit)
- Dilution Solution TYPE 1 contains sucrose for best osmolarity
- Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium
VitroGel® RGD High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions during the 3D cell culture. VitroGel® RGD High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection,
VitroGel makes it possible to bridge the in vitro and in vivo studies with the
same platform system.
Contents |
VitroGel® RGD
High Concentration, 3 mL |
VitroGel® Dilution
Solution, 50 mL |
|
Hydrogel
Formulation |
Xeno-free
tunable hydrogel modified with RGD peptide. |
HIGH
CONCENTRATION |
|
Use |
Good
for adhesion cells or cells requiring stronger cell-matrix interactions. |
Mix
& Match |
Can be blended with other versions of VitroGel® to create a multi-functional hydrogel |
Operation |
Room
temperature |
Hydrogel
Strength |
10 to
4,000 Pa of G’ depending on dilution ratio. |
Dilute with VitroGel® Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
|
pH |
Neutral |
Color |
Transparent |
Cell
Harvesting |
20 min cell recovery using VitroGel® Cell Recovery Solution |
Injectable |
Injectable
hydrogel |
Storage |
Store
at 2-8°C. Ships at ambient temperature |
Number
of Uses |
Dilution
ratio: |
1:2 =
225 uses at 50 µL per well |
|
1:3 =
300 uses at 50 µL per well |
|
1:5 =
450 uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® Angiogenesis Assay Kit
ready-to-use, hydrogel system for 2D gel coating and 3D
culture of angiogenesis tube formation, invasion, and animal injection.
- Hydrogel - Hydrogel Only: VitroGel® AAK (2 mL)
- Type 1 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 1 (500 µL), AAK Supplement 2 (500 µL)
- Type 2 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 1 (2 x 500 µL)
- Type 3 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 2 (2 x 500 µL)
VitroGel Angiogenesis Assay Kit
- Xeno-free hydrogel system supporting angiogenesis process
- Multiple applications in one kit: Tube formation, invasion, and animal injection
- Control the hydrogel properties: Add your own growth factors and compare with positive control
- Easy, efficient, and enzyme-free cell harvesting in 20 minutes
VitroGel® Angiogenesis Assay Kit is a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesis process. The kit can be used to study the angiogenesis tube formation and invasion on both 2D hydrogel coating method and 3D cell culture method. The VitroGel® system is also good for animal injection for in vivo study.
Angiogenesis is a highly regulated process that involves the growth of new blood vessels from the existing vasculature. This process plays an important role in both normal developmental processes and numerous pathologies, including wound healing, tumor growth, and metastasis to inflammation and ocular disease.
Traditional angiogenesis assay highly relies on natural extracellular matrix (natural ECM), which has non-adjustable hydrogel compositions and properties. Therefore, our understanding of the angiogenesis process is limited by studying the molecular cues such as growth factors and inhibitors in culture medium only. There is a lack of knowledge on how the properties of hydrogel affect the angiogenesis process.
There are two versions of VitroGel® Angiogenesis Assay Kits:
- VitroGel® Angiogenesis Assay Kit (Ready-To-Use): Ready-to-use with a fixed hydrogel mechanical strength to support the angiogenesis assay with adjustable supplements
- VitroGel® Angiogenesis Assay HC Kit (Cat No. TWG011-K): Assay kit with a tunable high concentration hydrogel to allow full control of the hydrogel’s mechanical strength with adjustable supplements.
Depending on the kit type, the ready-to-use VitroGel®
Angiogenesis Assay Kits can contain the following components:
- VitroGel® AAK, a xeno-free ready-to-use hydrogel.
- AAK Supplement 1, a hydrogel supplement without vascular endothelial growth factors (VEGFs) for cell attachment and growth.
- AAK Supplement 2, a hydrogel supplement with VEGFs to promote tube formation and as a positive control.
The VitroGel® AAK hydrogel is room temperature stable and
can be directly mixed with each supplement at 2:1 (v/v) ratio for hydrogel
formation. Researchers can adjust the
hydrogel’s molecular cues by adding the growth factors/inhibitors directly to
the supplements before mixing with the VitroGel® AAK hydrogel. Cells cultured in this system can be
harvested further easily with the VitroGel® Cell Recovery Solution.
Contents and Specifications
TYPE
1 Kit Contents |
VitroGel® AAK
(2 mL) |
AAK
Supplement 1 (1 x 500 µL) |
|
AAK
Supplement 2 (1 x 500 µL) |
|
TYPE
2 Kit Contents |
VitroGel® AAK
(2 mL) |
AAK
Supplement 1 (2 x 500 µL) |
|
TYPE
3 Kit Contents |
VitroGel® AAK
(2 mL) |
AAK
Supplement 2 (2 x 500 µL) |
|
Formulation |
Ready-to-use,
xeno-free functional hydrogel |
AAK
Supplement 1: Without VEGFs |
|
AAK
Supplement 2: With VEGFs |
|
Use |
Angiogenesis
Assay, tube formation, invasion, animal injection |
Biocompatibility |
Biocompatible,
safe for animal studies |
Injection |
Injectable
hydrogel for in vivo studies and lab automation |
Cell
Harvesting |
Easy
cell recovery using VitroGel® Cell Recovery Solution |
pH |
Neutral |
Shipping |
Supplements
require dry ice shipment. |
Storage |
VitroGel® AAK
hydrogel: store at 2-8°C |
AAK
Supplement 1 and AAK Supplement 2: store at -20°C |
|
Number
of Uses |
60
uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® ORGANOID
ready-to-use, xeno-free (animal origin-free) hydrogel system
for organoid culture
- Xeno-free hydrogel for organoid formation
- Supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources
- Scalable protocol for lab automation
- Easy 20-minute cell harvesting (enzyme-free)
- VitroGel® ORGANOID (1-4) are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model.
VitroGel® ORGANOID hydrogels are ready to use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel® ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.
The VitroGel® ORGANOID hydrogels can work in conjunction with VitroGel® STEM (a xeno-free hydrogel for 3D static suspension cultures and scale-up of human pluripotent stem cells) by transferring stem cell spheroids cultured in VitroGel® STEM to VitroGel® ORGANOID hydrogels for organoid differentiation. See Xeno-free 3D Organoid Workflow B. Key growth factors and molecules can be directly mixed with the hydrogel matrix or by adding on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. VitroGel® ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.
Which organoid hydrogel to choose
We still strongly suggest starting with the VitroGel®
ORGANOID Discovery Kit (Item # VHM04-K) to make a quick screening to find the
hydrogel version that is optimal for the organoid conditions.
The kit includes all four types of organoid hydrogels:
VitroGel® ORGANOID-1, VitroGel® ORGANOID-2, VitroGel® ORGANOID-3, VitroGel® ORGANOID-4)
Each of the four types has various bio-functional ligands,
mechanical strengths, and degradability to fulfill the needs of different
organoid culture conditions.
Because there are a wide range of organoid cell resources
(from stem cells, patient-derived tissue, co-culture, to PDX), it is hard to
tell which organoid hydrogel would best perform the researcher’s experiment.
Therefore, the Discovery Kit helps researchers to screen the four different
formulations to determine the best organoid hydrogel version for the organoid
conditions.
From our findings, versions 1, 2, or 3 are suitable for
gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2
or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for
cancer organoids.
Specifications
Formulation |
Xeno-free,
functional hydrogel |
Use |
Organoid
culture |
Operation |
Ready-to-use
at room temperature |
Biocompatibility |
Biocompatible,
safe for animal studies |
Injection |
Injectable
hydrogel for in vivo studies and lab automation |
Cell
Harvesting |
Easy cell recovery using VitroGel® Cell Recovery Solution |
pH |
Neutral |
Storage |
Store
at 2-8°C. Ships at ambient temperature |
Sizes |
Single
Vial: 10 mL |
Discovery
Kit: (2 mL) each of ORGANOID-1, ORGANOID-2, ORGANOID-3, ORGANOID-4 |
|
Number
of Uses |
(10
mL) 300 uses at 50 µL per well |
(2
mL) 60 uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
AAK Supplement
Hydrogel supplements for use with the VitroGel® Angiogenesis Assay Kits
- AAK Supplement 1 - Without VEGFs (Dry Ice Shipping Required)
- AAK Supplement 2 - With VEGFs (Dry Ice Shipping Required)
Specifications
Formulation |
AAK
Supplement 1: |
Without
VEGFs |
|
AAK
Supplement 2: |
|
With
VEGFs |
|
Use |
Angiogenesis
Assay, tube formation, invasion, animal injection |
Storage |
Store
at -20°C |
Shipping |
Requires
dry ice shipment. |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® MSC
Mesenchymal Stem Cell 3D Culture and Scale-up
VitroGel® MSC is a xeno-free (animal origin-free) hydrogel system developed to support three-dimensional (3D) cultures of mesenchymal stem cells (MSCs). This hydrogel system can be used to make hydrogel cell beads for MSC scale-up. Microcarriers are not required for MSC scale-up.
VitroGel® MSC is ready-to-use with an optimized formulation that fully supports the rapid expansion of MSCs. Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D culture or hydrogel-cell bead generation. This hydrogel system is compatible with most MSC culture media and tissue culture vessels. By using the VitroGel® Cell Recovery Solution, cell harvesting after 3D culture is simple and efficient.
3D cell culture process in 20 min – “Just add cells”
VitroGel® MSC is ready-to-use. Just mix with your cells.
Specifications
Formulation |
Xeno-free, functional
hydrogel |
Use |
3D cell culture, 2D
hydrogel coating, hydrogel-cell bead formation |
Operation |
Ready-to-use at room
temperature |
Biocompatibility |
Biocompatible, safe for
animal studies |
Cell Harvesting |
Easy cell recovery
using VitroGel® Cell Recovery Solution |
pH |
Neutral |
Storage |
Store at 2-8°C. Ships at
ambient temperature |
Sizes |
10 mL and 2 mL |
Number of Uses |
(10 mL) 300 uses at 50 µL
per well |
(2 mL) 60 uses at 50 µL
per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® MMP High Concentration
Matrix metalloproteinases (MMP) sensitive biodegradable
hydrogel – tunable, xeno-free hydrogel, high concentration (3 mL kit)
- Dilution Solution TYPE 1 contains sucrose for best osmolarity
- Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium
VitroGel® MMP High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with matrix metalloproteinase-sensitive peptides. The enzyme-sensitive hydrogel can be degraded by several MMPs (MMP1, MMP2, MMP3, MMP9, and MMP13) and support different biological activities such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, etc. VitroGel® MMP High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection,
VitroGel® makes it possible to bridge the in vitro and in vivo studies with the
same platform system.
Contents |
VitroGel® MMP
High Concentration, 3 mL |
VitroGel® Dilution
Solution, 50 mL |
|
Hydrogel
Formulation |
Xeno-free
tunable hydrogel modified with matrix metalloproteinases sensitive peptide. |
HIGH
CONCENTRATION |
|
Use |
MMP
sensitive biodegradable hydrogel. |
Mix
& Match |
Can
be blended with other versions of VitroGel to create a multi-functional
hydrogel |
Operation |
Room
temperature |
Hydrogel
Strength |
10 to
4,000 Pa of G’ depending on dilution ratio. |
Dilute
with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different
concentrations. |
|
pH |
Neutral |
Color |
Transparent |
Cell
Harvesting |
20
min cell recovery using VitroGel® Cell Recovery Solution |
Injectable |
Injectable
hydrogel |
Storage |
Store
at 2-8°C. Ships at ambient temperature |
Number
of Uses |
Dilution
ratio: |
1:2 =
225 uses at 50 µL per well |
|
1:3 =
300 uses at 50 µL per well |
|
1:5 =
450 uses at 50 µL per well |
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.