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ActionsFor any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® IKVAV High Concentration
Laminin-derived functional peptide (IKVAV) modified –
tunable, xeno-free hydrogel, high concentration (3 mL kit)
- Dilution Solution TYPE 1 contains sucrose for best osmolarity
- Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium
VitroGel® IKVAV High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (IKVAV). IKVAV is the bioactive sequence located on the C-terminal end of the long arm of the α-1 chain of laminin, which is actively involved in different biological activities such as neuronal progenitor cell differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth. VitroGel® IKVAV High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.
Specifications
Contents |
VitroGel® RGD
High Concentration, 3 mL |
VitroGel® Dilution
Solution, 50 mL |
|
Hydrogel
Formulation |
Xeno-free
tunable hydrogel modified with RGD peptide. |
HIGH
CONCENTRATION |
|
Use |
Good
for adhesion cells or cells requiring stronger cell-matrix interactions. |
Mix
& Match |
Can be blended with other versions of VitroGel® to create a multi-functional hydrogel |
Operation |
Room
temperature |
Hydrogel
Strength |
10 to
4,000 Pa of G’ depending on dilution ratio. |
Dilute with VitroGel® Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
|
pH |
Neutral |
Color |
Transparent |
Cell
Harvesting |
20 min cell recovery using VitroGel® Cell Recovery Solution |
Injectable |
Injectable
hydrogel |
Storage |
Store
at 2-8°C. Ships at ambient temperature |
Number
of Uses |
Dilution
ratio: |
1:2 =
225 uses at 50 µL per well |
|
1:3 =
300 uses at 50 µL per well |
|
1:5 =
450 uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® YIGSR High Concentration
Laminin-derived functional peptide (YIGSR) modified –
tunable, xeno-free hydrogel, high concentration (3 mL kit)
- Dilution Solution TYPE 1 contains sucrose for best osmolarity
- Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium
VitroGel® YIGSR High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (YIGSR). YIGSR sequence resides on the laminin β1 chain, which involves endothelial cell adhesion, cell proliferation and motility/migration. VitroGel® YIGSR High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection,
VitroGel® makes it possible to bridge the in vitro and in vivo studies with the
same platform system.
Contents |
VitroGel® YIGSR
High Concentration, 3 mL |
VitroGel® Dilution
Solution, 50 mL |
|
Hydrogel
Formulation |
Xeno-free
tunable hydrogel modified with YIGSR peptide. HIGH CONCENTRATION |
Use |
Support
endothelial cell adhesion, cell proliferation and motility/migration |
Mix
& Match |
Can
be blended with other versions of VitroGel® to create a multi-functional
hydrogel |
Operation |
Room
temperature |
Hydrogel
Strength |
10 to
4,000 Pa of G’ depending on dilution ratio. |
Dilute
with VitroGel® Dilution Solution (TYPE 1 or TYPE 2) for different
concentrations. |
|
pH |
Neutral |
Color |
Transparent |
Cell
Harvesting |
20
min cell recovery using VitroGel® Cell Recovery Solution |
Injectable |
Injectable
hydrogel |
Storage |
Store
at 2-8°C. Ships at ambient temperature |
Number
of Uses |
Dilution
ratio: |
1:2 =
225 uses at 50 µL per well |
|
1:3 =
300 uses at 50 µL per well |
|
1:5 =
450 uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans. To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® COL High Concentration
collagen-mimetic functional hydrogel – tunable, xeno-free (3
mL)
- Dilution Solution TYPE 1 contains sucrose for best osmolarity
- Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium
VitroGel® COL High Concentration is a tunable, xeno-free hydrogel system that mimics the functions of native collagen. The hydrogel can promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo, which shows great potential for tissue engineering and regeneration medicine application. VitroGel® COL High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.
Contents |
VitroGel® COL
High Concentration, 3 mL |
VitroGel® Dilution
Solution, 50 mL |
|
Hydrogel
Formulation |
Xeno-free
tunable collagen-mimetic functional hydrogel. HIGH CONCENTRATION |
Use |
Support
integrin binding to promote osteoblastic differentiation in vitro and
enhancing osteoblastic activity in vivo |
Mix
& Match |
Can
be blended with other versions of VitroGel to create a multi-functional
hydrogel |
Operation |
Room
temperature |
Hydrogel
Strength |
10 to
4,000 Pa of G’ depending on dilution ratio. |
Dilute
with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different
concentrations. |
|
pH |
Neutral |
Color |
Transparent |
Cell
Harvesting |
20
min cell recovery using VitroGel Cell Recovery Solution |
Injectable |
Injectable
hydrogel |
Storage |
Store
at 2-8°C. Ships at ambient temperature |
Number
of Uses |
Dilution
ratio: |
1:2 =
225 uses at 50 µL per well |
|
1:3 =
300 uses at 50 µL per well |
|
1:5 =
450 uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® Dilution Solution (50 mL)
For hydrogel concentration adjustment with the high
concentration hydrogels.
- Type 1 - Contains sucrose to maintain the best osmolarity.
- Type 2 - Sucrose-free formulation for cells sensitive to the sugar level in the medium.
VitroGel® Dilution Solution is a ready-to-use solution to be used with the VitroGel® High Concentration hydrogels to adjust the hydrogel concentration for different hydrogel strength.
The solution is room temperature stable with a neutral pH,
which maintains a good physiological condition for hydrogel formation and can
accelerate the hydrogel formation in combination with the cell medium. The
Dilution Solution can be used to prepare a thin gel coating plate or injectable
hydrogel with the high concentrations hydrogels.
Specifications
- Ready-to-use to adjust the concentration of VitroGel® High Concentration hydrogels
- Neutral pH
- Room temperature stable
- Transparent
- Compatible with VitroGel® system for 2D & 3D cell culture and hydrogel injection
- Ships and store at room temperature
- Size: 50 mL
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® HEK293
Ready-to-use, xeno-free (animal origin-free) hydrogel system
for 3D culture of HEK293 cells
VitroGel® HEK293 is a xeno-free (animal origin-free) functional hydrogel system developed to support three-dimensional (3D) cultures of human embryonic kidney 293 (HEK293) cells.
- Simple 5-minute protocol
- High-quality 3D spheroids
- Easily scale up with flasks and bioreactors
- Easy cell harvesting protocol (Enzyme-free, No trypsin)
VitroGel® HEK293 is a unique system to easily support the
rapid 3D growth of HEK293 cells. Cells
directly thawed from liquid nitrogen or passaged from 2D culture vessels can be
immediately mixed with the hydrogel solution for 3D static suspension cultures.
Both our standard 3D cell culture protocol and the 3D static suspension culture
protocols can be used for rapid cell expansion. The high-quality 3D spheroids
enhance the cell performance for protein expression and downstream
applications. HEK293 cells cultured in VitroGel® HEK293 can easily be scale-up
for large-scale bioreactors or spin flasks. By using the VitroGel® Cell
Recovery Solution, the cell harvesting after 3D culture is simple and
effective.
Formulation |
Xeno-free,
functional hydrogel |
Use |
3D
cell culture, 2D hydrogel coating, hydrogel-cell bead formation |
Operation |
Ready-to-use
at room temperature |
Biocompatibility |
Biocompatible,
safe for animal studies |
Injection |
Injectable
hydrogel for in vivo studies and lab automation |
Cell
Harvesting |
Easy
cell recovery using VitroGel® Cell Recovery Solution |
pH |
Neutral |
Storage |
Store
at 2-8°C. Ships at ambient temperature |
Sizes |
10 mL
and 2 mL |
Number
of Uses |
(10
mL) 300 uses at 50 µL per well |
(2
mL) 60 uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
GelNest™ Matrix
GelNest™ Matrix is derived from mouse tumor tissue and contains extracellular matrix components such as laminin, type IV collagen, heparan sulfate proteoglycans, and more. These components support cell adhesion, differentiation, and proliferation, providing signals for these processes. Additionally, they simulate the characteristics of the basement membrane in the physiological environment, enhancing the success rate and effectiveness of cell culture.
In addition to the matrix components, GelNest™ Matrix is rich in various growth factors. These growth factors promote cell differentiation, proliferation, and migration, mimicking cellular signaling pathways and interactions in the physiological environment. GelNest™ Matrix has a wide range of applications, particularly in tissue engineering, cell culture, and research. It can be used for organoid culture, stem cell differentiation, angiogenesis, migration or invasion assays, and in vivo tumor studies.
Storage and Operation Instructions
To store GelNest™ Matrix, it is recommended to keep it in a -20°C freezer until ready for use. Prior to initial use, thaw the gel and divide it into single-use portions. Store these portions in a -80°C freezer for a maximum of 2 years. Please note that GelNest™ Matrix Gel should not be stored in a frost-free freezer.
GelNest™ Matrix is in a liquid state at 4°C and transitions into a gel state at 37°C. It starts to solidify into a gel when the temperature exceeds 10°C. For optimal performance, it is advised to pre-chill the pipette tips and conduct the procedures on ice.
GelNest™ Matrix Quality Assurance
- The protein concentration is guaranteed to be between 8 - 20 mg / mL
- The gel formation performance remains stable
- There are no LDEV (Lactate Dehydrogenase Elevated Virus), bacteria, or mycoplasma present for a high safety performance
- The endotoxin level is < 10 EU / mL
- Tests for organ-like structures and stem cell culture have been successfully performed.
- Tests for angiogenesis, tumor invasion, and tumor formation have been successfully conducted
Specifications
Item | Growth Factor | Phenol Red | Recommended Applications | Volume |
211212 | Normal | Yes | General 2D, 3D cell culture | 5 mL |
211222 | Normal | No | Requires colorimetric identification (such as fluorescence) or sensitivity to steroids | 5 mL |
211232 | Low | Yes | 2D, 3D cell culture with higher accuracy requirements for matrix components and requires | 5 mL |
211242 | Low | No | Colorimetric identification or sensitivity to steroids | 5 mL |
211252 | Normal | Yes | In vivo tumor formation, thrombosis test, angiogenesis experiment, general cell culture, etc. | 5 mL |
211262 | Normal | No | 2D, 3D cell culture with higher accuracy requirements for matrix components | 5 mL |
211272 | Low | Yes | hESC stem cell culture | 5 mL |
211282 | Low | No | Organoid culture and differentiation | 5 mL |
211492 | Normal | Yes | Angiogenesis Experiment | 5 mL |
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® Hydrogel Matrix
ready-to-use, xeno-free (animal origin-free) functional
hydrogel system
- Xeno-free, biological functional hydrogel
- Ready-to-use at room temperature (20 min protocol)
- Supports a wide range of cell types and applications
- Easy, efficient, and enzyme-free cell harvesting in 20 min
VitroGel® Hydrogel Matrix is a ready-to-use, xeno-free (animal origin-free) functional hydrogel for 3D cell culture research. VitroGel® Hydrogel Matrix is an optimized formulation of multi-functional ligands and concentration to support a wide range of cell types for different applications.“Just Add Cells” – The hydrogel matrix is ready to mix with cell suspension directly.
There is no additional adjustment needed. VitroGel® Hydrogel Matrix closely mimics the natural extracellular matrix (ECM) environment to make cells feel more like at home. The hydrogel is room temperature stable, has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. This user-friendly functional hydrogel creates an excellent balance of simplicity and versatility.
3D cell culture process in 20 min – “Just add cells”
VitroGel® Hydrogel Matrix is ready-to-use. Just mix with
your cells. There is no cross-linking agent or the need to adjust the hydrogel
concentration.
Specifications
Formulation |
Xeno-free,
functional hydrogel |
Use |
3D
and 2D cell culture |
Operation |
Ready-to-use
at room temperature |
Biocompatibility |
Biocompatible,
safe for animal studies |
Injection |
Injectable
hydrogel for in vivo studies and lab automation |
Cell
Harvesting |
20
min cell recovery using VitroGel® Cell Recovery Solution |
pH |
Neutral |
Storage |
Store
at 2-8°C. Ships at ambient temperature |
Sizes |
10 mL
and 2 mL |
Number
of Uses |
(10
mL) 300 uses at 50 µL per well |
(2
mL) 60 uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
ig BL21 (DE3)
Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.
Specifications
Competent cell type: Chemically competent
Derivative of: BL21(DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: 1.0 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes
Shipping condition: Dry ice
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Reagents Needed for One Reaction
BL21(DE3) Chemically Competent Cells: 50 µl
DNA (or pUC19 Control, 10 pg/µl): 1 µl
Recovery Medium: 1 ml
Contents & Storage
BL21(DE3) Competent Cells: -80 °C
pUC19 Control DNA: -20 °C
Recovery Medium: 4 °C
Genomic Features
BL21(DE3) chemically competent cells have the following features:
- Widely used host background
- T7 Expression Strain
- Deficient in both lon (1) and ompT proteases
- Resistant to phage T1 (fhuA2)
- B Strain
Genotype
F-ompT hsdS(rB- mB-) gal dcm (DE3)
General Guidelines
Follow these guidelines when using BL21(DE3) chemically competent E. coli.
- Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
- Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.
Transformation Protocol
- Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
- Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
- When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl)
- DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping.
- Incubate the cells with DNA on ice for 30 minutes.
- After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
- Transfer the tubes to ice for 2 minutes.
- Add 950 µl of Recovery Medium or any other medium of choice to each tube.
- Incubate tubes at 37 °C for 1 hour at 210 rpm.
- Spread 50 μl to 200 μl from each transformation on pre-warmed selection plates.
- Incubate the plates overnight at 37 °C.
ig 5-Alpha
Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.
- Up to 2x higher efficiency than any competitor
- Exact same Genotype as NEB's 5-Alpha Cells
Number of Colonies per Transformation Test*
Specifications
Competent cell type: Chemically competent
Derivative of: 5-alpha
Species: E. coli
Format: Tubes
Transformation efficiency: 1 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes
Shipping condition: Dry ice
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Reagents Needed for One Reaction
ig 5-alpha chemically competent cells: 50 µl
DNA (or pUC19 Control, 10 pg/µl): 1 µl
Recovery medium: 1 ml
Contents & Storage
ig 5-alpha competent cells: -80 °C
pUC19 control DNA: -20 °C
Recovery medium: 4 °C
Genotype
80 (lacZ)M15 fhuA2 (argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17
General Guidelines
Follow these guidelines when using ig 5-alpha chemically competent cells.
- Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
- Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.
Protocol
- Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
- Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
- When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl) DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping. 4) Incubate the cells with DNA on ice for 30 minutes.
- After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
- Transfer the tubes to ice for 2 minutes.
- Add 950 µl of Recovery Medium or any other medium of choice to each tube.
- Incubate tubes at 37 °C for 1 hour at 210 rpm.
- Spread 50 μl to 200 μl from each transformation on Pre-warmed selection plates.
- Incubate the plates overnight at 37 °C.
ig 10B
Intact Genomics ig 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.
- Most efficient chemically competent cells in the industry (up to 10X more efficient!)
- May easily be used in place of NEB 10-Beta, TOP10 from Invitrogen or other common brands
- Significantly more affordable
Specifications
Competent cell type: Chemically competent
Derivative of: DH10B
Species: E. coli
Format: Tubes
Transformation efficiency: â¥1.0 x 1010 cfu/µg pUC19 DNA
Blue/white screening: Yes
Shipping condition: Dry ice
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Reagents Needed for One Reaction
ig 10B chemically competent cells: 50 µl
DNA (or pUC19 Control, 10 pg/µl): 1 µl
Recovery medium: 1 ml
Contents & Storage
- ig 10B competent cells: -80 °C
- pUC19 control DNA: -20 °C
- Recovery medium: 4 °C
Genomic Features
ig 10B (DH10B derivative) chemically competent cells have the following features:
- 80lacZM15 marker provides α-complementation of the β-galactosidase gene with blue/white screening
- mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
Genotype
F â mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 80dlacZM15 lacX74 araD139 (ara, leu)7697 galU galK rpsL (StrR) nupG -
General Guidelines
Follow these guidelines when using ig 10B chemically competent E. coli.
- Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
- Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.
Protocol
- Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
- Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
- When the cells are thawed, add 50 µl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl) DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping.
- Incubate the cells with DNA on ice for 30 minutes.
- After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
- Transfer the tubes to ice for 2 minutes.
- Add 950 µl of Recovery Medium or any other medium of choice to each tube.
- Incubate tubes at 37 °C for 1 hour at 210 rpm.
- Spread 50 µl to 200 µl from each transformation on Pre-warmed selection plates.
- Incubate the plates overnight at 37 °C.
VitroINK® Bioinks
Ready-to-use bioinks for 3D bioprinting
VitroINK® are ready-to-use, xeno-free (animal origin-free) bioink system either unmodified or modified with either RGD, Collagen-memetic, IKVAV, or YIGSR peptides or with Matrix Metalloproteinases (MMP) for biodegradable bioink.
VitroINK® is a family of the ready-to-use, xeno-free tunable bioink system that requires no UV, no temperature/pH curing, or chemical cross-linking. The system is ready-to-use at room temperature, neutral in pH, transparent, and has excellent visibility after printing and cell culture. Due to the unique shear-thinning and rapid recovery mechanical property, VitroINK® can maintain the printed structure without UV or other special curing methods. Adding cell culture medium after printing can further stabilize the printed structure and support cell growth. Cells can be pre-mix with VitroINK® using our VitroINK® Mixing Kit for mixing ratios at 3:1 or 10:1. Different versions of VitroINK® may incorporate multiple biological functional ligands to promote cell attachment, cell-matrix interactions, cell proliferation, motility/migration and differentiation for many different applications.
Specifications
- Xeno-free tunable modified bioink.
- Ready-to-use at room temperature
- No UV, temperature/pH curing, or chemical cross-linking required
- Neutral pH
- Transparent. Excellent visibility after printing and cell culture
- Pre-mix with cells using the included VitroINK® Mixing Kit
- Ships room temperature. Store at 2-8°C
- Size: 3 mL
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroINK® 10:1 Mixing Component Pack
Single-use components for 10:1 mixing ratio for VitroINK®
Mixing Kit. DISPENSER NOT INCLUDED
The VitroINK® 10:1 Mixing Component Kit includes a 1 mL syringe, connector and mixing head to replace the one-time-use components in the full VitroINK® Mixing Kit – Complete Pack (Item # IMK00-1) for 10:1 VitroINK® and cells mixing.
This package is sterilized and disposable for one-time use.
Kit Contents and Specifications
- Disposable mixing kit components for VitroINK® kit:
- (1) 1 mL syringe
- (1) connector and tubing
- (1) mixing head
- Sterile
- Use for 10:1 mixing ratio
- Ships room temperature. Store at room temperature.
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.