Azure Nitrocellulose Membranes

Pre-cut Nitrocellulose membrane for Western blotting

Low-fluorescence Nitrocellulose membranes are available in both 0.45 μm and 0.22 μm pore sizes to accommodate larger and smaller proteins, respectively. We recommend using the .45 µm pore size for the most analytical blotting: protein, ssDNA, and RNA transfers.

• Low molecular weight proteins
• Typically used for chemiluminescence
• Not ideal for stripping
• Excellent binding properties

Quantity: 10 membranes each   
 
 
 

Azure PVDF Membranes

Pre-cut PVDF membranes for Western blotting applications

Ideal for chemiluminescent and colorimetric Western blotting. Pre-cut to save you time in the lab. These low-fluorescence PVDF membranes reduce background noise and nonspecific protein binding for improved sensitivity. Available in three different pre-cut sizes.

Quantity: 10 membranes per package

Features

Low-fluorescence PVDF membranes reduce background noise for improved sensitivity. PVDF (polyvinylidene difluoride) membranes are commonly used for immunoblotting techniques, like Western blots. During the blotting process, samples are transferred from the protein gel onto a PVDF membrane by electroblotting. From there they can be used for protein sequencing.

• High binding capacity is great for low-expressing proteins
• Typically used for fluorescence
• High protein retention for stripping and reprobing

Azure Blotting Paper for Western Blotting

Pre-cut blotting paper for Western blot transfer

Forget about measuring and cutting blotting paper to the size of your mini-gels. Use these pre-cut papers to assemble your transfer “sandwiches”. Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow.

Qty: 60 sheets

Blotting papers are cut to completely cover the gels and membranes.

For 8x10cm gels: blotting papers available in 9x13cm

For 7x9cm gels: blotting papers available in 8x10cm

Features

Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow. Forget about measuring and cutting blotting paper to the size of your mini-gels! Use these pre-cut papers to assemble your transfer “sandwiches”.

Blotting papers come pre-cut to completely cover the gels and membranes.   
 
 
 

Cytiva 3MM Chr paper is the world's most widely used blotting paper.

This acceptance and usage reflect the high quality, purity and consistency that are relied upon by researchers doing Southern, Northern and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) used extensively for blotting in electrophoresis for lifting of sequencing gels.

GB004
A thick gel blotting paper (1.0 mm), used for wicking purposes only. Provides higher absorbency and more consistent wicking than paper towels. Recommended for applications where fewer layers of gel blotting paper must still ensure a high capacity. Fewer layers of blotting paper reduce the risk of trapping air bubbles. Recommended for capillary blotting of nucleic acids.

GB005
An extra-thick (1.2 mm ), highly absorbent paper recommended for applications where fewer layers of blotting paper must siill ensure a high capacity. Recommended for semi-dry blotting of proteins.

17 Chr
A thick (0.92 mm) and highly absorbent paper.

31 ET
An extremely fast and thick paper (0.5 mm) with a fairly soft surface.

Features and Benefits

• Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps
• Manufactured and tested specifically for chromatographic techniques. This ensures the wicking capability and uniformity of capillary action that is important in obtaining clean and even transfers during blotting
• Cytiva 3MM Chr is considered the industry standard for blotting procedures
• Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows "out-of-the-box" usage and eliminates sheet-to-sheet variations

3MW is used widely in nucleic acid and protein research for blotting, wicking, spacers, and gel drying due to its purity, quality and consistent results. Uniform capillary action provides even wicking and enables gel transfers to be lifted RELIABLY from glass supports.

Cytiva 3MM Chr paper is the world's most widely used blotting paper.

This acceptance and usage reflect the high quality, purity and consistency that are relied upon by researchers doing Southern, Northern and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) used extensively for blotting in electrophoresis for lifting of sequencing gels.

GB004
A thick gel blotting paper (1.0 mm), used for wicking purposes only. Provides higher absorbency and more consistent wicking than paper towels. Recommended for applications where fewer layers of gel blotting paper must still ensure a high capacity. Fewer layers of blotting paper reduce the risk of trapping air bubbles. Recommended for capillary blotting of nucleic acids.

GB005
An extra-thick (1.2 mm ), highly absorbent paper recommended for applications where fewer layers of blotting paper must siill ensure a high capacity. Recommended for semi-dry blotting of proteins.

17 Chr
A thick (0.92 mm) and highly absorbent paper.

31 ET
An extremely fast and thick paper (0.5 mm) with a fairly soft surface.

Features and Benefits

• Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps
• Manufactured and tested specifically for chromatographic techniques. This ensures the wicking capability and uniformity of capillary action that is important in obtaining clean and even transfers during blotting
• Cytiva 3MM Chr is considered the industry standard for blotting procedures
• Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows "out-of-the-box" usage and eliminates sheet-to-sheet variations

Protran nitrocellulose membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate, which will lower the protein binding capacity. In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantees superior signal-to-noise ratios, without the need for stringent washing conditions. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins that prefer aqueous environments. Prior to transfer, the membrane is simply wet in water and then in the transfer buffer, with no other necessary pre-treatment steps. Protran membranes exhibit the best handling strangth of all pure nitrocellulose membranes.
Features:

• High binding capacity (80-150g/cm2) of proteins and nucleic acids.
• 100% pure nitrocellulose, with no cellulose acetate added, ensures the highest binding capacity possible.
• Compatible with virtually all standard detection methods.
• Low background - NC surface properties guarantee superior signal-to-noise ratios.
• Triton-free matrix with low extractable levels will support the cell growth essential for colony blotting, plaque lifts, and tissue culture applications.
• Available in many sizes and formats to fit different transfer devices and gel chambers.

Nitrocellulose Membranes

Combining the advantages of high protein binding capacity with low background and high membrane stability, which ensures easy handling.

Ideal for western blotting, DNA blotting as well as dot or slot blots. They have been optimized for all protein blotting systems, such as electro transfer, semi-dry or simple capillary blotting

Specifications:

Protran nitrocellulose membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate, which will lower the protein binding capacity. In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantees superior signal-to-noise ratios, without the need for stringent washing conditions. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins that prefer aqueous environments. Prior to transfer, the membrane is simply wet in water and then in the transfer buffer, with no other necessary pre-treatment steps. Protran membranes exhibit the best handling strangth of all pure nitrocellulose membranes.
Features:

• High binding capacity (80-150g/cm2) of proteins and nucleic acids.
• 100% pure nitrocellulose, with no cellulose acetate added, ensures the highest binding capacity possible.
• Compatible with virtually all standard detection methods.
• Low background - NC surface properties guarantee superior signal-to-noise ratios.
• Triton-free matrix with low extractable levels will support the cell growth essential for colony blotting, plaque lifts, and tissue culture applications.
• Available in many sizes and formats to fit different transfer devices and gel chambers.