Western Blot 2.0 with PURITY™

The PURITY™ detection system revolutionizes Western Blotting in three steps.
 

Blocking buffer and secondary antibody are already components of the PURITY™ Reagent. And the washing buffer is also included. The only thing to add is your specific primary antibody.

Benefits:

• 1 simple protocol which makes almost all optimization unnecessary.
• Increased sensitivity and reproducibility of blots.
• Dramatically reduces the number of steps and time required for Western blots.
• Drastically decreases background phenomena.
• Highly reliable.

• Used for agarose electrophoresis of DNA, RNA or nucleic acids
• Contains 3 tracking dyes and 15% Ficoll in a special Tris dye

• Light blue - around 4000bp in 1% agarose
• Indigo - around 600bp in 1% agarose
• Magenta - around 150bp in 1% agarose

• DNase/RNase/Protease free

IB01015
5X RNA Gel Loading Kit

• Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
• RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
• DNase/RNase/Protease free

IB01190
2X Protein Loading Dye

• Tracks the migration progression of your sample during polyacrylamide electrophoresis
• Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
• DNase/RNase/Protease free

IB72120
Xylene Cyanol FF

• Used as a tracking dye at 5kb to monitor the progress of electrophoresis separation
• CAS Number: 2650-17-1
• DNase/RNase/Protease free

IB74040
Bromophenol Blue

• Tracking dye in electrophoretic separations
• Migrates around 0.5kb
• CAS Number: 115-39-9
• Purity: >98.0%

RunBlue SDS Running Buffer (NXB50500) a TEO-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can be for reduced and non-reduced samples.

RunBlue TGS Blot Buffer(NXB82600) has been specially formulated to provide optimal blot transfer of proteins from RunBlue Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue Dual Run and Blot system or a factor of 20 for use with other blotting methods.

Cytiva 3MM Chr paper is the world's most widely used blotting paper.

This acceptance and usage reflect the high quality, purity and consistency that are relied upon by researchers doing Southern, Northern and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) used extensively for blotting in electrophoresis for lifting of sequencing gels.

GB004
A thick gel blotting paper (1.0 mm), used for wicking purposes only. Provides higher absorbency and more consistent wicking than paper towels. Recommended for applications where fewer layers of gel blotting paper must still ensure a high capacity. Fewer layers of blotting paper reduce the risk of trapping air bubbles. Recommended for capillary blotting of nucleic acids.

GB005
An extra-thick (1.2 mm ), highly absorbent paper recommended for applications where fewer layers of blotting paper must siill ensure a high capacity. Recommended for semi-dry blotting of proteins.

17 Chr
A thick (0.92 mm) and highly absorbent paper.

31 ET
An extremely fast and thick paper (0.5 mm) with a fairly soft surface.

Features and Benefits

• Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps
• Manufactured and tested specifically for chromatographic techniques. This ensures the wicking capability and uniformity of capillary action that is important in obtaining clean and even transfers during blotting
• Cytiva 3MM Chr is considered the industry standard for blotting procedures
• Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows "out-of-the-box" usage and eliminates sheet-to-sheet variations

3MW is used widely in nucleic acid and protein research for blotting, wicking, spacers, and gel drying due to its purity, quality and consistent results. Uniform capillary action provides even wicking and enables gel transfers to be lifted RELIABLY from glass supports.

Cytiva 3MM Chr paper is the world's most widely used blotting paper.

This acceptance and usage reflect the high quality, purity and consistency that are relied upon by researchers doing Southern, Northern and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) used extensively for blotting in electrophoresis for lifting of sequencing gels.

GB004
A thick gel blotting paper (1.0 mm), used for wicking purposes only. Provides higher absorbency and more consistent wicking than paper towels. Recommended for applications where fewer layers of gel blotting paper must still ensure a high capacity. Fewer layers of blotting paper reduce the risk of trapping air bubbles. Recommended for capillary blotting of nucleic acids.

GB005
An extra-thick (1.2 mm ), highly absorbent paper recommended for applications where fewer layers of blotting paper must siill ensure a high capacity. Recommended for semi-dry blotting of proteins.

17 Chr
A thick (0.92 mm) and highly absorbent paper.

31 ET
An extremely fast and thick paper (0.5 mm) with a fairly soft surface.

Features and Benefits

• Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps
• Manufactured and tested specifically for chromatographic techniques. This ensures the wicking capability and uniformity of capillary action that is important in obtaining clean and even transfers during blotting
• Cytiva 3MM Chr is considered the industry standard for blotting procedures
• Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows "out-of-the-box" usage and eliminates sheet-to-sheet variations

Protran nitrocellulose membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate, which will lower the protein binding capacity. In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantees superior signal-to-noise ratios, without the need for stringent washing conditions. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins that prefer aqueous environments. Prior to transfer, the membrane is simply wet in water and then in the transfer buffer, with no other necessary pre-treatment steps. Protran membranes exhibit the best handling strangth of all pure nitrocellulose membranes.
Features:

• High binding capacity (80-150g/cm2) of proteins and nucleic acids.
• 100% pure nitrocellulose, with no cellulose acetate added, ensures the highest binding capacity possible.
• Compatible with virtually all standard detection methods.
• Low background - NC surface properties guarantee superior signal-to-noise ratios.
• Triton-free matrix with low extractable levels will support the cell growth essential for colony blotting, plaque lifts, and tissue culture applications.
• Available in many sizes and formats to fit different transfer devices and gel chambers.

Nitrocellulose Membranes

Combining the advantages of high protein binding capacity with low background and high membrane stability, which ensures easy handling.

Ideal for western blotting, DNA blotting as well as dot or slot blots. They have been optimized for all protein blotting systems, such as electro transfer, semi-dry or simple capillary blotting

Specifications:

Protran nitrocellulose membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate, which will lower the protein binding capacity. In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantees superior signal-to-noise ratios, without the need for stringent washing conditions. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins that prefer aqueous environments. Prior to transfer, the membrane is simply wet in water and then in the transfer buffer, with no other necessary pre-treatment steps. Protran membranes exhibit the best handling strangth of all pure nitrocellulose membranes.
Features:

• High binding capacity (80-150g/cm2) of proteins and nucleic acids.
• 100% pure nitrocellulose, with no cellulose acetate added, ensures the highest binding capacity possible.
• Compatible with virtually all standard detection methods.
• Low background - NC surface properties guarantee superior signal-to-noise ratios.
• Triton-free matrix with low extractable levels will support the cell growth essential for colony blotting, plaque lifts, and tissue culture applications.
• Available in many sizes and formats to fit different transfer devices and gel chambers.