Azure PVDF Membranes

Pre-cut PVDF membranes for Western blotting applications

Ideal for chemiluminescent and colorimetric Western blotting. Pre-cut to save you time in the lab. These low-fluorescence PVDF membranes reduce background noise and nonspecific protein binding for improved sensitivity. Available in three different pre-cut sizes.

Quantity: 10 membranes per package

Features

Low-fluorescence PVDF membranes reduce background noise for improved sensitivity. PVDF (polyvinylidene difluoride) membranes are commonly used for immunoblotting techniques, like Western blots. During the blotting process, samples are transferred from the protein gel onto a PVDF membrane by electroblotting. From there they can be used for protein sequencing.

• High binding capacity is great for low-expressing proteins
• Typically used for fluorescence
• High protein retention for stripping and reprobing

Azure Blotting Paper for Western Blotting

Pre-cut blotting paper for Western blot transfer

Forget about measuring and cutting blotting paper to the size of your mini-gels. Use these pre-cut papers to assemble your transfer “sandwiches”. Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow.

Qty: 60 sheets

Blotting papers are cut to completely cover the gels and membranes.

For 8x10cm gels: blotting papers available in 9x13cm

For 7x9cm gels: blotting papers available in 8x10cm

Features

Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow. Forget about measuring and cutting blotting paper to the size of your mini-gels! Use these pre-cut papers to assemble your transfer “sandwiches”.

Blotting papers come pre-cut to completely cover the gels and membranes.   
 
 
 

Azure Flash Western Chemiluminescence Kit

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

Each kit includes:

• 10x PVDF Membranes
• 100mL Chemi Blot Blocking Buffer
• 500μL Flash HRP Conjugate
• 250mL Flash Wash Buffer
• 100mL Radiance ECL Substrate

Features

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

• Fast protocol with few hands-on steps
• Antibody stabilization for enhanced chemi signal
• Low background and increased signal-to-noise ratios
• Increased detection sensitivity and linear dynamic range

Hoefer® Nitrocellulose Membranes

• Ideal for Western Blotting and other immunoblotting techniques
• BSA protein binding capacity of 160 µg/cm2–can bind a wide range of fragment sizes and can still be easily blocked for low background
• High wick rates (lateral flow)–substances move quickly through the membrane

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Hoefer® PVDF (Polyvinylidene Fluoride) Membranes

• Ideal for use in protein binding applications such as western blots, solid phase assays, and immunoblotting procedures
• Multiple reprobings possible
• Superior strength–can withstand aggressive handling or be used with automated equipment without breaking or tearing
• Low extractables–ensures tests will be clean with consistent results
• Exceptional sensitivity detects low-level components
• Hydrophobic–resists water
• High binding capacity–binds a wide range of fragment sizes

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BluBlot™ HS Autoradiography Film for Western Blots

BluBlot™ HS Autoradiography Film is a premium, high-sensitivity film specifically designed for Western blotting, chemiluminescence detection, and a broad range of autoradiographic applications.

Manufactured with a medium/dark blue polyester base and a double-emulsion coating, this film delivers sharp, publication-quality images with exceptional clarity and contrast.

Compatible with a wide range of radioisotopes, including ¹⁴C, ³²P, ³³P, ¹²⁵I, and ³⁵S, BluBlot™ HS ensures high-resolution results and superior signal-to-noise ratios, making it ideal for sensitive detection methods such as chemiluminescence, fluorescence, and phosphor imaging.

This film performs reliably with both manual development techniques and automatic X-ray film processors, offering outstanding versatility in the lab. It is also suitable for use with blue-emitting rare-earth screens and calcium tungstate intensifying screens. Packaged in light-safe, lined dispenser boxes of 100 sheets, BluBlot™ HS is protected for long-term storage and offers a minimum 18-month shelf life from the date of production. It provides performance comparable to leading autoradiography films at a fraction of the cost.

Key Features

• High-sensitivity autoradiography film for Western blots and electrophoretic applications
• Compatible with ¹⁴C, ³²P, ³³P, ¹²⁵I, ³⁵S isotopes
• Optimized for chemiluminescence and radioactive detection
• Medium/dark blue base for enhanced image contrast
• Double emulsion for superior clarity and consistency
• Publication-quality images
• Works with automatic processors and manual development
• Long shelf life (18+ months)
• Cost-effective alternative to premium autoradiography films

Hoefer® Gradient Makers

Ideal for generating gradients of polyacrylamide, sucrose, and cesium chloride.

• Made of heavy acrylic plastic with high-quality, leak-free Teflon® valves
• Flat base provides stability on the magnetic stir. Comes with a support rod for attaching to a ring stand
• Takes Luer or 4 mm ID tubing. With included adapters, it can also take 2 mm ID or 22-gauge tubing

SG15, SG30, SG50 and SG100 include

• Gradient Maker with Teflon® Valves, 1/ea
• Adapter Barbed Fitting, 1/ea
• Luer Outlet Fitting, 1/ea
• 22-gauge Needle, 1/ea
• Support Rod, 1/ea 

The 500 mL SG500 Gradient Maker is made of two cylindrical acrylic chambers joined and mounted on a flat acrylic base.

• An easy-to-use push-pull valve opens and closes the passage between the reservoir and mixing chambers
• Suitable for forming polyacrylamide gradients or buffer gradients used with medium to small chromatography columns
• Ideal for use with the SE615 Multiple Gel Caster  

SG500 includes

• Gradient Maker with Push-Pull Valve, 1/ea
• Hose Clamps, 4/pk

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Hoefer® Gel Casters

Enable several gradient or homogeneous gels to be cast simultaneously. Choice of multiple-gel casters or dual-gel casters.

Gel Casters for Small Format Vertical Electrophoresis Systems (SE250, SE260, SE300)

Multiple-Gel Casters

• Pour uniform gels from the top; pump gradient gels into an inlet port at the bottom of the unit
• Sandwiches fill at the same time and at the same rate from the same solution
• The result: identical gels
• Glass plate sandwiches seal leak-free without the use of messy grease or tape
• Once cast, gels can be wrapped in plastic and refrigerated for several weeks

Dual-Gel Caster (SE245)

• Cast one or two gels
• Accommodates both 10 x 8 cm and 10 x 10.5 cm plates

*For use with the SE250 and SE260 Series only.
 

Gel Casters for SE600 and SE400 Series Vertical Units   

Multiple-Gel Casters

• SE675 casts up to four 16 cm long gels
• SE615 casts up to ten 16 cm long gels
• Easy to use and leak-free
• Cast homogeneous or gradient gels. Pour homogeneous gels directly into the top of the casting chamber: pump gradient gels through an inlet port at the bottom

Dual-Gel Caster (SE6015)

SE6015 Dual Gel Caster is included when you order an SE600 series unit. It can also be ordered separately.

• Casts two 8, 16, or 24 cm long gels at a time
• Double the capacity with optional divider plates
• Easy to use and leak-free
• Glass Sandwichers seal into the casting 

*For use with the SE600 Series only.

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Hoefer® Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation. Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution.

• High loading capacity: 50 uL max. sample volume
• High resolution: Upgraded bis-Tris system for clearer, sharper separation bands and higher resolution
• Ready to use and easy to store: No solution preparation or gel casting is required, saving time. Store at 2 to 8ºC
• Good Repeatability: Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability
• Highly effective: Max 40 min for electrophoresis
• Safe and non-toxic: No hazardous reagents need to be handled 

Compatible with Hoefer® Mighty Small II Vertical Electrophoresis Systems and the Hoefer® miniVE Vertical Protein Electrophoresis and Blotting Systems.  

Specifications
 

Item #	MSGT	MSGF
Plate Size	3.94 x 3.15 In. | 10 x 8 cm
Gel Size	3.35 x 2.76 In. | 8.5 x 7 cm, 1mm thickness
System/Buffer	Bis-Tris System, MOPS-SDS Buffer	Bis-Tris System, MOPS Buffer
Wells	12-well, 15-well
Storage	Store at 2 to 8°C. (35.6 to 46.4ºF) Do not freeze.
Concentration	Gradient Gels: 4 to 12%, 4 to 20% | Homogenous Gels: 10%, 12%
Loading Vol.	For 12 wells: max 50 uL (recommended: ≤ 25 uL) | For 15 wells: max 30 uL (recommended: ≤ 15 uL)

Hoefer® Combs for SUB15 and SUBHT Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

MC = Microtiter spacing for use with a multichannel pipette
Sample volume listed is for a 5 mm deep well

For use with SUB15 and SUBHT Submarine Gel Electrophoresis Systems

Specifications
 

Item #	Description	Wells	Teeth	Thickness	Width	Sample Vol.
SUB15-C1-10	SUB15 and SUBHT Comb	10 Wells	10 Teeth	1 mm	12.8 mm	64 uL
SUB15-C15-10	SUB15 and SUBHT Comb	10 Wells	10 Teeth	1.5 mm	12.8 mm	96 uL
SUB15-C2-10	SUB15 and SUBHT Comb	10 Wells	10 Teeth	2 mm	12.8 mm	128 uL
SUB15-C1-16MC	SUB15 and SUBHT Comb	16 Wells	16 Teeth	1 mm	6.4 mm	32 uL
SUB15-C15-16MC	SUB15 and SUBHT Comb	16 Wells	16 Teeth	1.5 mm	6.4 mm	48 uL
SUB15-C2-16MC	SUB15 and SUBHT Comb	16 Wells	16 Teeth	2 mm	6.4 mm	64 uL
SUB15-C1-20	SUB15 and SUBHT Comb	20 Wells	20 Teeth	1 mm	5.9 mm	29 uL
SUB15-C15-20	SUB15 and SUBHT Comb	20 Wells	20 Teeth	1.5 mm	5.9 mm	43 uL
SUB15-C2-20	SUB15 and SUBHT Comb	20 Wells	20 Teeth	2 mm	5.9 mm	58 uL
SUB15-C1-25	SUB15 and SUBHT Comb	25 Wells	25 Teeth	1 mm	3.5 mm	17 uL
SUB15-C15-25	SUB15 and SUBHT Comb	25 Wells	25 Teeth	1.5 mm	3.5 mm	25 uL
SUB15-C2-25	SUB15 and SUBHT Comb	25 Wells	25 Teeth	2 mm	3.5 mm	34 uL
SUB15-C1-30MC	SUB15 and SUBHT Comb	30 Wells	30 Teeth	1 mm	2.5 mm	12 uL
SUB15-C15-30MC	SUB15 and SUBHT Comb	30 Wells	30 Teeth	1.5 mm	2.5 mm	18 uL
SUB15-C2-30MC	SUB15 and SUBHT Comb	30 Wells	30 Teeth	2 mm	2.5 mm	24 uL

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Hoefer® Combs for SUB20 and SUB20C Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

• MC = Microtiter spacing for use with a multichannel pipette
• Sample volume listed is for a 5 mm deep well
• For use with SUB20 and SUB20C Submarine Gel Electrophoresis Systems

Specifications
 

Item #	Description	Wells	Teeth	Thickness	Width	Sample Vol.
SUB20-C1-16	SUB20 and SUB20C Comb	16 Wells	16 Teeth	1 mm	8.5 mm	35 uL
SUB20-C15-16	SUB20 and SUB20C Comb	16 Wells	16 Teeth	1.5 mm	8.5 mm	55 uL
SUB20-C2-16	SUB20 and SUB20C Comb	16 Wells	16 Teeth	2 mm	8.5 mm	75 uL
SUB20-C1-20MC	SUB20 and SUB20C Comb	20 Wells	20 Teeth	1 mm	7 mm	30 uL
SUB20-C15-20MC	SUB20 and SUB20C Comb	20 Wells	20 Teeth	1.5 mm	7 mm	45 uL
SUB20-C2-20MC	SUB20 and SUB20C Comb	20 Wells	20 Teeth	2 mm	7 mm	60 uL
SUB20-C1-28	SUB20 and SUB20C Comb	28 Wells	28 Teeth	1 mm	5 mm	20 uL
SUB20-C15-28	SUB20 and SUB20C Comb	28 Wells	28 Teeth	1.5 mm	5 mm	30 uL
SUB20-C2-28	SUB20 and SUB20C Comb	28 Wells	28 Teeth	2 mm	5 mm	40 uL
SUB20-C1-40MC	SUB20 and SUB20C Comb	40 Wells	40 Teeth	1 mm	3 mm	13 uL
SUB20-C15-40MC	SUB20 and SUB20C Comb	40 Wells	40 Teeth	1.5 mm	3 mm	19 uL
SUB20-C2-40MC	SUB20 and SUB20C Comb	40 Wells	40 Teeth	2 mm	3 mm	25 uL

 

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Hoefer® Combs for SUB10 Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

• MC = Microtiter spacing for use with a multichannel pipette
• Sample volume listed is for a 5 mm deep well
• For use with SUB10 Submarine Gel Electrophoresis Systems

Specifications

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