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PR1MA™ Autoradiography and Chemiluminescence Film for Western Blots
One autoradiography film for ALL your needs!
Many companies sell film solely on price without considering your needs. Some offer many different films making it difficult to decide what is best for your needs.
MIDSCI™ narrowed the Autoradiography Film field to PR1MA™ Blue Sensitive Film. It will work outstandingly well for almost 100% of all molecular labs' needs.
- PR1MA™ Autoradiography Film is double emulsion
- High signal sensitivity
- For autoradiography of 32P, 33P, 35S, 125I, and 14C
- Excellent lot-to-lot consistency
- Exceptional clarity with extremely low backgrounds
- Consistently superior results for exposure of blotting experiments and sequencing gels
- Publishable grade autoradiographs
- Optimized for chemiluminescence
- Film works with calcium tungstate or blue rare earth screens
- For use manually or with automatic processing
- 100 sheets per box
- Available in 3 sizes: 5 x 7 in., 8 x 10 in., and 14 x 17 in.
Specifications
PR1MA™ Blue Film BX | |||
Item # | Size | qty | Features |
BX57 | 5 x 7 in. | 100/pk | Double Emulsion |
BX810 | 8 x 10 in. | 100/pk | |
BX1417 | 14 x 17 in. | 100/pk, 5pks/cs |
Autoradiography, and, Chemiluminescence, Film, Western, blots, PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
PR1MA™ Rapid Coomassie Stain
- Appearance: Dark Blue Color
- Concentration: 20X
- Solubility: Suitable for use in staining protein in polyacrylamide gels
Rapid, Commassie, Stain, PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
PR1MA™ Protein Gel-Loading Dye for SDS-PAGE
- Appearance: Slightly Viscous Dark Purple Liquid
- DNase, RNase, protease: Not Detected
- Electrophoresis: Pass Test
- OPTICAL ABS AT 525 NM: 0.15 - 0.35
- OPTICAL ABS BETWEEN 588-594 NM: 0.32 - 0.47
- OPTICAL ABS BETWEEN 635-641 NM: 0.25 - 0.40
Protein, Gel-Loading, Dye, SDS-PAGE,PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
Aqua Transfer Cell
Perform a quick 1 hour transfer or transfer overnight at a lower voltage
Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.
Includes:
- 2 transfer cassettes
- 6 sponges
- 2 Azure Aqua cooling units
- Tank and upper lid with power cables
- Transfer Electrode Cores
Features
The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.
Chemical reagent compatibility
Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.
Cleaning Instructions
Before using, wash all components with a neutral detergent and rinse well with ultra pure water.
Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.
Specifications
Parameters | Azure Aqua Transfer Cell Specs |
Number of cassettes | 2 |
Buffer requirement | 1100 mL |
Electrode distance | 4 cm |
Electrode assembly size ( L x W x H) | 17.5 cm x 13.5 cm x 19 cm |
Transfer time | 60 minutes |
Cooling | Azure Aqua cooling unit |
Blotting area | 7.5 cm x 10 cm |
Buffer volume (with cooling unit) | 650 mL |
Buffer volume (without cooling unit) | 850 mL |
Unit dimensions (W x L x H) | 13.5 cm x 17.5 cm x 19 cm |
Tank buffer volume | 1100 mL |
Maximum gel size | 7.5 cm x 10 cm |
Gel capacity | 2 precast or handcast gels |
Weight | 1.8 lbs |
Recommended power supply | Azure Aqua Power Supply 600V |
Azure ChemiWriter ECL Pen
Chemiluminescent pen for blot annotation
The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.
Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
Quantity: 1 pen
Features
Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.
- Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
- There are two heads to the pen, a fine point tip and a thicker chisel point tip.
- We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
- The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
- For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
- Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
- Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.
Azure Aqua Power Supply
Designed for powering electrophoresis and Western blotting transfer modules
This power supply can create and save protocols for all your gel and blotting experiments. Designed with you in mind, features a pause/resume function for flexibility.
Power supply includes output range of 5–600V, 1–1200mA, 1–500W, LCD readable voltage, current, power, and four power connections to run multiple experiments at once. Able to control constant power, current, or voltage.
Features
The Azure Aqua Power Supply is a universal power supply that is designed for powering electrophoresis and transfer modules. The power supply can create and save protocols for all your gel and blotting experiments and includes a pause/resume function for flexibility.
Azure Power Supply includes an output range of 5-600V, 1-1200mA, 1-500W, LCD readable voltage, current, power, and 4 connections for electrophoresis and transfer.
Specifications
Parameters | Azure Aqua Power Supply 600V Specs |
Output specifications | 600V, 1.2 A, 500W |
Output range (programmable) | 10-600V 10-1200mA1-500W |
Type of output | Constant voltage, current, and power |
Timer control | 1 min – 99 hr 59 min |
Pause/resume function | Yes |
Output terminals | 4 connections |
Unit dimensions (W x L x H) | 24 x 30.5 x 10 cm |
Weight | 5.6 lbs |
Stackable | Yes |
Regulatory | CE |
Safety features | Automatic monitoring of over-voltage, no load, sudden change of load, overload, and short circuit |
Azure Western Blot Quenching Sheets
Background quenching sheets for use with fluorescent or chemiluminescent samples
Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.
- For use with fluorescent or chemiluminescent samples.
- Size: 9 x 12.5 cm
- Quantity: 10 Sheets
Features
Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.
The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.
Azure Aqua Quad Mini-Cell
Vertical Gel Running System
Designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis. The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels.
Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize. Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.
Includes:
- 4 gel casting stands and casting frames buffer dam
- 5 spacer plates (1mm)
- 10 short glass plates
- 5 15-well combs
- 5 10-well combs
- 5 sealing gaskets
- 5 gel shovels
- Tank and lid with power cables
- Primary and Secondary Electrode Cores
Features
- The Azure Aqua Quad Mini-Cell is designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis.
- The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels: Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize.
- Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.
Specifications
Parameters | Azure Aqua Quad Mini-Cell Specs |
Unit dimensions (W x L x H) | 13.5 cm x 17.5 cm x 19 cm |
Weight | 2.2 lbs |
Recommended power supply | Azure Aqua Power Supply 600V |
Number of gels | 1-4 precast (most major brands) or handcast gels |
Tank buffer volume | 2 gels: 540 mL | 4 gels: 1100 mL |
Maximum gel size | 6.7 cm x 8.6 cm |
Typical run times | 30-45 minutes at 200V |
Gel cassette size (WxL) | 10 cm x 8 cm |
Azure Nitrocellulose Membranes
Pre-cut Nitrocellulose membrane for Western blotting
Low-fluorescence Nitrocellulose membranes are available in both 0.45 μm and 0.22 μm pore sizes to accommodate larger and smaller proteins, respectively. We recommend using the .45 µm pore size for the most analytical blotting: protein, ssDNA, and RNA transfers.
- Low molecular weight proteins
- Typically used for chemiluminescence
- Not ideal for stripping
- Excellent binding properties
Quantity: 10 membranes each
Azure PVDF Membranes
Pre-cut PVDF membranes for Western blotting applications
Ideal for chemiluminescent and colorimetric Western blotting. Pre-cut to save you time in the lab. These low-fluorescence PVDF membranes reduce background noise and nonspecific protein binding for improved sensitivity. Available in three different pre-cut sizes.
Quantity: 10 membranes per package
Features
Low-fluorescence PVDF membranes reduce background noise for improved sensitivity. PVDF (polyvinylidene difluoride) membranes are commonly used for immunoblotting techniques, like Western blots. During the blotting process, samples are transferred from the protein gel onto a PVDF membrane by electroblotting. From there they can be used for protein sequencing.
- High binding capacity is great for low-expressing proteins
- Typically used for fluorescence
- High protein retention for stripping and reprobing
Azure Blotting Paper for Western Blotting
Pre-cut blotting paper for Western blot transfer
Forget about measuring and cutting blotting paper to the size of your mini-gels. Use these pre-cut papers to assemble your transfer “sandwiches”. Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow.
Qty: 60 sheets
Blotting papers are cut to completely cover the gels and membranes.
For 8x10cm gels: blotting papers available in 9x13cm
For 7x9cm gels: blotting papers available in 8x10cm
Features
Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow. Forget about measuring and cutting blotting paper to the size of your mini-gels! Use these pre-cut papers to assemble your transfer “sandwiches”.
Blotting papers come pre-cut to completely cover the gels and membranes.
Azure Flash Western Chemiluminescence Kit
Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol
Each kit includes:
- 10x PVDF Membranes
- 100mL Chemi Blot Blocking Buffer
- 500μL Flash HRP Conjugate
- 250mL Flash Wash Buffer
- 100mL Radiance ECL Substrate
Features
Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.
The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.
- Fast protocol with few hands-on steps
- Antibody stabilization for enhanced chemi signal
- Low background and increased signal-to-noise ratios
- Increased detection sensitivity and linear dynamic range