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PR1MA™ Autoradiography and Chemiluminescence Film for Western Blots

 

One autoradiography film for ALL your needs!


Many companies sell film solely on price without considering your needs. Some offer many different films making it difficult to decide what is best for your needs.

MIDSCI™ narrowed the Autoradiography Film field to PR1MA™ Blue Sensitive Film. It will work outstandingly well for almost 100% of all molecular labs' needs.
 

  • PR1MA™ Autoradiography Film is double emulsion
  • High signal sensitivity
  • For autoradiography of 32P, 33P, 35S, 125I, and 14C
  • Excellent lot-to-lot consistency
  • Exceptional clarity with extremely low backgrounds
  • Consistently superior results for exposure of blotting experiments and sequencing gels
  • Publishable grade autoradiographs
  • Optimized for chemiluminescence
  • Film works with calcium tungstate or blue rare earth screens
  • For use manually or with automatic processing
  • 100 sheets per box
  • Available in 3 sizes: 5 x 7 in., 8 x 10 in., and 14 x 17 in.

 

Specifications

 

PR1MA™ Blue Film BX
Item #SizeqtyFeatures
BX575 x 7 in.100/pkDouble Emulsion
BX8108 x 10 in.100/pk
BX141714 x 17 in.100/pk, 5pks/cs

 


Item#:
ASFILM

PR1MA™ Rapid Coomassie Stain


Chemical Formula: C5H10O5

Solubility: Water

Storage Temperature: Room Temperature


This unique Coomassie Blue based formulation features a special dye that is absorbed by protein bands instead of the gel matrix. This eliminates the need for destaining and offers enhanced sensitivity and sharper resolution of bands. Protein bands can be detected within 10 minutes. Maximum intensification may require incubation up to 40 minutes. Offers greater convenience and sensitivity than conventional stains. Compatible with silver staining. Rapid Coomassie stain uses a simple two-step procedure. Stain is designed to detect as little as 7.9ng of protein within one hour of electrophoresis.

Supplied as 20X concentrated solution.

Shelf life is approximately 18 months at room temperature.

Shipping Weight: 0.3 lbs

Shipping Dimensions: 2.00 x 4.00 x 2.00

  • Appearance: Dark Blue Color
  • Concentration: 20X
  • Solubility: Suitable for use in staining protein in polyacrylamide gels

Research or further manufacturing use only, not for food or drug use.

PR1MA™ Protein Gel-Loading Dye for SDS-PAGE

CAS Number: 7732-18-5; 56-81-5; 7647-01-0; 77-86-1; 151-21-3

Storage Temperature: Room Temperature

This dye is added to protein samples prior to electrophoresis on SDS–PAGE gels.

Composition:

Water: 80%
Glycerol: <12%
Sodium Dodecyl Sulfate: <5%
Proprietary Component: <2%
TRIS: <1.0%
Shipping Weight: 0.1 lbs

Shipping Dimensions: 1.50 x 3.50 x 1.50
  • Appearance: Slightly Viscous Dark Purple Liquid
  • DNase, RNase, protease: Not Detected
  • Electrophoresis: Pass Test
  • OPTICAL ABS AT 525 NM: 0.15 - 0.35
  • OPTICAL ABS BETWEEN 588-594 NM: 0.32 - 0.47
  • OPTICAL ABS BETWEEN 635-641 NM: 0.25 - 0.40

Research or further manufacturing use only, not for food or drug use.
Item#:
ASPROTEINGELLDNSDS

Aqua Transfer Cell

 

Perform a quick 1 hour transfer or transfer overnight at a lower voltage

Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.

 

Includes:

  • 2 transfer cassettes
  • 6 sponges
  • 2 Azure Aqua cooling units
  • Tank and upper lid with power cables
  • Transfer Electrode Cores

 

Features

 

The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.

 

Chemical reagent compatibility

 

Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.

 
Cleaning Instructions

 

Before using, wash all components with a neutral detergent and rinse well with ultra pure water.

Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.

 

Specifications
 

ParametersAzure Aqua Transfer Cell Specs
Number of cassettes2
Buffer requirement1100 mL
Electrode distance4 cm
Electrode assembly size ( L x W x H)17.5 cm x 13.5 cm x 19 cm
Transfer time60 minutes
CoolingAzure Aqua cooling unit
Blotting area7.5 cm x 10 cm
Buffer volume (with cooling unit)650 mL
Buffer volume (without cooling unit)850 mL
Unit dimensions (W x L x H)13.5 cm x 17.5 cm x 19 cm
Tank buffer volume 1100 mL
Maximum gel size7.5 cm x 10 cm
Gel capacity2 precast or handcast gels
Weight1.8 lbs
Recommended power supplyAzure Aqua Power Supply 600V

 

 

Item#:
ASAZAQUATRANCELL

Azure ChemiWriter ECL Pen

 

Chemiluminescent pen for blot annotation

The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.

Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.


Quantity: 1 pen

 

Features

Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.

  • Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
  • There are two heads to the pen, a fine point tip and a thicker chisel point tip.
  • We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
  • The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
  • For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
  • Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
  • Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.
 
Item#:
ASAZCHEMIPEN

Azure Aqua Power Supply

 

Designed for powering electrophoresis and Western blotting transfer modules

This power supply can create and save protocols for all your gel and blotting experiments. Designed with you in mind, features a pause/resume function for flexibility.

 

Power supply includes output range of 5–600V, 1–1200mA, 1–500W, LCD readable voltage, current, power, and four power connections to run multiple experiments at once. Able to control constant power, current, or voltage.

 

Features

The Azure Aqua Power Supply is a universal power supply that is designed for powering electrophoresis and transfer modules. The power supply can create and save protocols for all your gel and blotting experiments and includes a pause/resume function for flexibility.

Azure Power Supply includes an output range of 5-600V, 1-1200mA, 1-500W, LCD readable voltage, current, power, and 4 connections for electrophoresis and transfer.

 

Specifications
 

ParametersAzure Aqua Power Supply 600V Specs
Output specifications600V, 1.2 A, 500W
Output range (programmable)10-600V 10-1200mA1-500W
Type of outputConstant voltage, current, and power
Timer control1 min – 99 hr 59 min
Pause/resume functionYes
Output terminals4 connections
Unit dimensions (W x L x H)24 x 30.5 x 10 cm
Weight5.6 lbs
StackableYes
RegulatoryCE
Safety featuresAutomatic monitoring of over-voltage, no load, sudden change of load, overload, and short circuit

 

Item#:
ASAZAQUAPOWSUP

Azure Western Blot Quenching Sheets

 

Background quenching sheets for use with fluorescent or chemiluminescent samples

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.

  • For use with fluorescent or chemiluminescent samples.
  • Size: 9 x 12.5 cm
  • Quantity: 10 Sheets

Features

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.

The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.




Item#:
ASAZQUENCH

Azure Aqua Quad Mini-Cell

 

Vertical Gel Running System

Designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis. The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels.

Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize. Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

 

Includes:

  • 4 gel casting stands and casting frames buffer dam
  • 5 spacer plates (1mm)
  • 10 short glass plates
  • 5 15-well combs
  • 5 10-well combs
  • 5 sealing gaskets
  • 5 gel shovels
  • Tank and lid with power cables
  • Primary and Secondary Electrode Cores

 

Features

  • The Azure Aqua Quad Mini-Cell is designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis.
  • The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels: Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize.
  • Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

 


Specifications
 
ParametersAzure Aqua Quad Mini-Cell Specs
Unit dimensions (W x L x H)13.5 cm x 17.5 cm x 19 cm
Weight2.2 lbs
Recommended power supplyAzure Aqua Power Supply 600V
Number of gels1-4 precast (most major brands) or handcast gels
Tank buffer volume2 gels: 540 mL | 4 gels: 1100 mL
Maximum gel size6.7 cm x 8.6 cm
Typical run times30-45 minutes at 200V
Gel cassette size (WxL)10 cm x 8 cm

 

Item#:
ASAZAQUADMINI

Azure Nitrocellulose Membranes

 

Pre-cut Nitrocellulose membrane for Western blotting

Low-fluorescence Nitrocellulose membranes are available in both 0.45 μm and 0.22 μm pore sizes to accommodate larger and smaller proteins, respectively. We recommend using the .45 µm pore size for the most analytical blotting: protein, ssDNA, and RNA transfers.

 

  • Low molecular weight proteins
  • Typically used for chemiluminescence
  • Not ideal for stripping
  • Excellent binding properties

 

Quantity: 10 membranes each   
 
 
 

Item#:
ASPCNITROMEM

Azure PVDF Membranes

 

Pre-cut PVDF membranes for Western blotting applications

Ideal for chemiluminescent and colorimetric Western blotting. Pre-cut to save you time in the lab. These low-fluorescence PVDF membranes reduce background noise and nonspecific protein binding for improved sensitivity. Available in three different pre-cut sizes.

 

Quantity: 10 membranes per package

 

Features

Low-fluorescence PVDF membranes reduce background noise for improved sensitivity. PVDF (polyvinylidene difluoride) membranes are commonly used for immunoblotting techniques, like Western blots. During the blotting process, samples are transferred from the protein gel onto a PVDF membrane by electroblotting. From there they can be used for protein sequencing.

  • High binding capacity is great for low-expressing proteins
  • Typically used for fluorescence
  • High protein retention for stripping and reprobing

 

Item#:
ASAZPVDFMEM

Azure Blotting Paper for Western Blotting

 

Pre-cut blotting paper for Western blot transfer

Forget about measuring and cutting blotting paper to the size of your mini-gels. Use these pre-cut papers to assemble your transfer “sandwiches”. Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow.

 

Qty: 60 sheets

Blotting papers are cut to completely cover the gels and membranes.

For 8x10cm gels: blotting papers available in 9x13cm

For 7x9cm gels: blotting papers available in 8x10cm

 

Features


Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow. Forget about measuring and cutting blotting paper to the size of your mini-gels! Use these pre-cut papers to assemble your transfer “sandwiches”.

Blotting papers come pre-cut to completely cover the gels and membranes.   
 
 
 

Item#:
ASAZBLOTPAPE

Azure Flash Western Chemiluminescence Kit

 

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

 

Each kit includes:

  • 10x PVDF Membranes
  • 100mL Chemi Blot Blocking Buffer
  • 500μL Flash HRP Conjugate
  • 250mL Flash Wash Buffer
  • 100mL Radiance ECL Substrate

Features

 

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

 

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

  • Fast protocol with few hands-on steps
  • Antibody stabilization for enhanced chemi signal
  • Low background and increased signal-to-noise ratios
  • Increased detection sensitivity and linear dynamic range

 

 

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