PR1MA™ Autoradiography and Chemiluminescence Film for Western Blots

One autoradiography film for ALL your needs!

Many companies sell film solely on price without considering your needs. Some offer many different films making it difficult to decide what is best for your needs.

MIDSCI™ narrowed the Autoradiography Film field to PR1MA™ Blue Sensitive Film. It will work outstandingly well for almost 100% of all molecular labs' needs.
 

• PR1MA™ Autoradiography Film is double emulsion
• High signal sensitivity
• For autoradiography of ³²P, ³³P, ³⁵S, ¹²⁵I, and ¹⁴C
• Excellent lot-to-lot consistency
• Exceptional clarity with extremely low backgrounds
• Consistently superior results for exposure of blotting experiments and sequencing gels
• Publishable grade autoradiographs
• Optimized for chemiluminescence
• Film works with calcium tungstate or blue rare earth screens
• For use manually or with automatic processing
• 100 sheets per box
• Available in 3 sizes: 5 x 7 in., 8 x 10 in., and 14 x 17 in.

Specifications

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New Look, Same Reliable Product - Now from PR1MA™!

PR1MA™ Western Blotting Boxes

Minimize Antibody Waste & Maximize Efficiency.

No matter the size of your blot, there’s a PR1MA™ Western Blotting Box designed to fit it perfectly. Our selection includes boxes tailored for mini, half, whole, and strip blots, ensuring the most efficient use of space and reagents. By matching blot size to the correct container, you can reduce reagent and antibody consumption, save money, and maximize shaker or rocker capacity during incubation.

All boxes feature uniformly flat bottoms and 90º angled corners to ensure even reagent distribution and minimal pooling. For light-sensitive samples, opaque black blotting boxes are available to protect antibodies and blots from photodegradation.

With a hinged lid to minimize evaporation and sizes optimized for all common blotting applications, these boxes are a must-have for any lab performing Western blot analysis.

Key Features

• Sized to fit all common blot formats – mini, half, whole, and strip blots
• Reduce antibody and reagent usage with perfectly sized boxes
• Flat bottom and 90° corners for uniform reagent coverage
• Hinged lids reduce evaporation during incubations
• Light-blocking black boxes available for light-sensitive protocols
• Ideal for Western blotting, membrane staining, and antibody incubations 

Use the right-sized box to get better results while saving on expensive reagents.   

Specifications

PR1MA™ Rapid Coomassie Stain

Proudly made in the USA! Click here for more made in America products!
  

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PR1MA™ Protein Gel-Loading Dye for SDS-PAGE

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Gel Crusher™ Spin Column

Rapid Gel Fragmentation for DNA Recovery.

Gel Crusher™ Spin Columns provide a fast, efficient, and consistent method for gel fragmentation, replacing traditional crush-and-soak or manual gel breaker steps. Designed with 600 uM filtration pores, these columns enable quick and uniform fragmentation of agarose or acrylamide gel slices, saving time and increasing DNA recovery yields.

Ideal for workflows in molecular cloning, PCR cleanup, restriction digests, DNA sequencing, and labeling protocols, the Gel Crusher™ simplifies the preparation of gel fragments for downstream purification and elution steps.

Simply insert the Gel Crusher™ column into a 2 mL microcentrifuge tube, add your pre-cut gel slice (prepared using a gel cutter or razor blade), and centrifuge at max speed (~15,000 x g) for 5 minutes. The gel will be forced through the 600 uM pores, producing small, uniform fragments that can be easily pipetted using wide-bore tips into NaCl or other solutions.

This process creates a fine slurry that enhances elution efficiency and improves access to the target nucleic acids, significantly reducing incubation and processing time.

Key Features

• Efficiently breaks gel slices into uniform fragments
• Eliminates manual crush-and-soak steps
• Increases DNA yield and recovery efficiency
• Fits standard 2 mL microcentrifuge tubes
• Compatible with standard benchtop centrifuges (up to 15,000 x g)
• 600 uM pore size ideal for agarose or acrylamide gels
• Simplifies downstream processes: elution, filtration, incubation
• For use in PCR, cloning, digestion, sequencing, and more 

Reversible Racks

• Choose 60 well (5 x 12) or 96 well (8 x 12) formats
• Reversible racks, accept 0.5 and 1.5 to 2.0 mL microtubes
• Alphanumeric marking on each well
• Made of polypropylene

Blu-Lite UHC™ Autoradiography Film for Western Blots

Blu-Lite UHC™ Autoradiography Film is a high-performance, ultra-high contrast (UHC) film designed for Western blotting, electrophoretic applications, and autoradiography detection. Engineered with a light blue polyester base and double-emulsion coating, Blu-Lite UHC™ offers exceptional sensitivity, low background, and crisp, publication-quality images.

Its specially formulated coating ensures an ideal balance between high sensitivity and low background noise, resulting in superior contrast for DNA, RNA, and protein visualization. The lighter blue tone enhances visibility and image clarity, making it especially effective for chemiluminescence-based detection methods.

Blu-Lite UHC™ is compatible with common radioisotopes, including ¹⁴C, ³²P, ¹²⁵I, and ³⁵S, making it versatile for a range of molecular biology applications. It works seamlessly with both manual film development and automatic X-ray film processors, ensuring ease of use in any lab setting.

With a shelf life of up to 2 years, Blu-Lite UHC™ is conveniently packaged in lined, light-safe dispenser boxes of 100 sheets, featuring an improved lift-off cover for easy access. Offering performance comparable to premium brands at a fraction of the cost, Blu-Lite UHC™ is a smart, reliable choice for consistent, high-quality autoradiographic results.

Key Features

• Ultra-high contrast (UHC) film with low background noise
• High sensitivity and sharp image production
• Optimized for chemiluminescence and radioactive detection
• Compatible with ¹⁴C, ³²P, ¹²⁵I, and ³⁵S isotopes
• Double-emulsion coating for consistent results
• Lighter blue film base for enhanced image clarity
• Works with both automatic processors and manual development
• Shelf life of 2 years from production date
• Packaged in light-safe dispenser boxes (100 sheets/box) with lift-off cover
• Cost-effective alternative to leading autoradiography film brands

Azure Aqua Transfer Cell

Perform a quick 1 hour transfer or transfer overnight at a lower voltage

Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.

Includes:

• 2 transfer cassettes
• 6 sponges
• 2 Azure Aqua cooling units
• Tank and upper lid with power cables
• Transfer Electrode Cores

Features

The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.

Chemical reagent compatibility

Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.

 
Cleaning Instructions

Before using, wash all components with a neutral detergent and rinse well with ultra pure water.

Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.

Specifications
 

Azure ChemiWriter ECL Pen

Chemiluminescent pen for blot annotation

The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.

Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.

Quantity: 1 pen

Features

Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.

• Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
• There are two heads to the pen, a fine point tip and a thicker chisel point tip.
• We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
• The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
• For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
• Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
• Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.

Azure Aqua Power Supply

Designed for powering electrophoresis and Western blotting transfer modules

This power supply can create and save protocols for all your gel and blotting experiments. Designed with you in mind, features a pause/resume function for flexibility.

Power supply includes output range of 5–600V, 1–1200mA, 1–500W, LCD readable voltage, current, power, and four power connections to run multiple experiments at once. Able to control constant power, current, or voltage.

Features

The Azure Aqua Power Supply is a universal power supply that is designed for powering electrophoresis and transfer modules. The power supply can create and save protocols for all your gel and blotting experiments and includes a pause/resume function for flexibility.

Azure Power Supply includes an output range of 5-600V, 1-1200mA, 1-500W, LCD readable voltage, current, power, and 4 connections for electrophoresis and transfer.

Specifications
 

Azure Western Blot Quenching Sheets

Background quenching sheets for use with fluorescent or chemiluminescent samples

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.

• For use with fluorescent or chemiluminescent samples.
• Size: 9 x 12.5 cm
• Quantity: 10 Sheets

Features

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.

The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.

Azure Aqua Quad Mini-Cell

Vertical Gel Running System

Designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis. The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels.

Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize. Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

Includes:

• 4 gel casting stands and casting frames buffer dam
• 5 spacer plates (1mm)
• 10 short glass plates
• 5 15-well combs
• 5 10-well combs
• 5 sealing gaskets
• 5 gel shovels
• Tank and lid with power cables
• Primary and Secondary Electrode Cores

Features

• The Azure Aqua Quad Mini-Cell is designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis.
• The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels: Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize.
• Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

Specifications
 

Parameters	Azure Aqua Quad Mini-Cell Specs
Unit dimensions (W x L x H)	13.5 cm x 17.5 cm x 19 cm
Weight	2.2 lbs
Recommended power supply	Azure Aqua Power Supply 600V
Number of gels	1-4 precast (most major brands) or handcast gels
Tank buffer volume	2 gels: 540 mL | 4 gels: 1100 mL
Maximum gel size	6.7 cm x 8.6 cm
Typical run times	30-45 minutes at 200V
Gel cassette size (WxL)	10 cm x 8 cm

 

Azure Nitrocellulose Membranes

Pre-cut Nitrocellulose membrane for Western blotting

Low-fluorescence Nitrocellulose membranes are available in both 0.45 μm and 0.22 μm pore sizes to accommodate larger and smaller proteins, respectively. We recommend using the .45 µm pore size for the most analytical blotting: protein, ssDNA, and RNA transfers.

• Low molecular weight proteins
• Typically used for chemiluminescence
• Not ideal for stripping
• Excellent binding properties

Quantity: 10 membranes each