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PR1MA Rapid Coomassie Stain

Chemical Formula: C5H10O5

Solubility: Water

Storage Temp: Room Temperature

This unique Coomassie Blue based formulation features a special dye that is absorbed by protein bands instead of the gel matrix. This eliminates the need for destaining and offers enhanced sensitivity and sharper resolution of bands. Protein bands can be detected within 10 minutes. Maximum intensification may require incubation up to 40 minutes. Offers greater convenience and sensitivity than conventional stains. Compatible with silver staining. Rapid Coomassie stain uses a simple two-step procedure. Stain is designed to detect as little as 7.9ng of protein within one hour of electrophoresis.

Supplied as 20X concentrated solution.

Shelf life is approximately 18 months at room temperature.

Shipping Weight: 0.3 lbs

Shipping Dimensions: 2.00 x 4.00 x 2.00
  • Appearance: Dark Blue Color
  • Concentration: 20X
  • Solubility: Suitable for use in staining protein in polyacrylamide gels
Research or further manufacturing use only, not for food or drug use.
 

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Protein Gel-Loading Dye for SDS-PAGE

CAS Number: 7732-18-5; 56-81-5; 7647-01-0; 77-86-1; 151-21-3
Storage Temp: Room Temperature

This dye is added to protein samples prior to electrophoresis on SDS–PAGE gels.

Composition:

Water: 80%
Glycerol: <12%
Sodium Dodecyl Sulfate: <5%
Proprietary Component: <2%
TRIS: <1.0%
Shipping Weight: 0.1 lbs

Shipping Dimensions: 1.50 x 3.50 x 1.50
  • Appearance: Slightly Viscous Dark Purple Liquid
  • DNase, RNase, protease: Not Detected
  • Electrophoresis: Pass Test
  • OPTICAL ABS AT 525 NM: 0.15 - 0.35
  • OPTICAL ABS BETWEEN 588-594 NM: 0.32 - 0.47
  • OPTICAL ABS BETWEEN 635-641 NM: 0.25 - 0.40
Research or further manufacturing use only, not for food or drug use.

Proudly made in the USA! Click here for more made in America products!
 
 

Item#:
ASPROTEINGELLDNSDS


Gel Crusher Spin Column

 

Rapid Gel Fragmentation for DNA Recovery.

 

Gel Crusher Spin Columns provide a fast, efficient, and consistent method for gel fragmentation, replacing traditional crush-and-soak or manual gel breaker steps. Designed with 600 uM filtration pores, these columns enable quick and uniform fragmentation of agarose or acrylamide gel slices, saving time and increasing DNA recovery yields.

 

Ideal for workflows in molecular cloning, PCR cleanup, restriction digests, DNA sequencing, and labeling protocols, the Gel Crusher simplifies the preparation of gel fragments for downstream purification and elution steps.

 

Simply insert the Gel Crusher column into a 2 mL microcentrifuge tube, add your pre-cut gel slice (prepared using a gel cutter or razor blade), and centrifuge at max speed (~15,000 x g) for 5 minutes. The gel will be forced through the 600 uM pores, producing small, uniform fragments that can be easily pipetted using wide-bore tips into NaCl or other solutions.

 

This process creates a fine slurry that enhances elution efficiency and improves access to the target nucleic acids, significantly reducing incubation and processing time.

 

Key Features

  • Efficiently breaks gel slices into uniform fragments
  • Eliminates manual crush-and-soak steps
  • Increases DNA yield and recovery efficiency
  • Fits standard 2 mL microcentrifuge tubes
  • Compatible with standard benchtop centrifuges (up to 15,000 x g)
  • 600 uM pore size ideal for agarose or acrylamide gels
  • Simplifies downstream processes: elution, filtration, incubation
  • For use in PCR, cloning, digestion, sequencing, and more 

Reversible Racks

  • Choose 60 well (5 x 12) or 96 well (8 x 12) formats
  • Reversible racks, accept 0.5 and 1.5 to 2.0 mL microtubes
  • Alphanumeric marking on each well
  • Made of polypropylene

Azure Aqua Transfer Cell

 

Perform a quick 1 hour transfer or transfer overnight at a lower voltage

Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.

 

Includes:

  • 2 transfer cassettes
  • 6 sponges
  • 2 Azure Aqua cooling units
  • Tank and upper lid with power cables
  • Transfer Electrode Cores

 

Features

 

The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.

 

Chemical reagent compatibility

 

Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.

 
Cleaning Instructions

 

Before using, wash all components with a neutral detergent and rinse well with ultra pure water.

Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.

 

Specifications
 

ParametersAzure Aqua Transfer Cell Specs
Number of cassettes2
Buffer requirement1100 mL
Electrode distance4 cm
Electrode assembly size ( L x W x H)17.5 cm x 13.5 cm x 19 cm
Transfer time60 minutes
CoolingAzure Aqua cooling unit
Blotting area7.5 cm x 10 cm
Buffer volume (with cooling unit)650 mL
Buffer volume (without cooling unit)850 mL
Unit dimensions (W x L x H)13.5 cm x 17.5 cm x 19 cm
Tank buffer volume 1100 mL
Maximum gel size7.5 cm x 10 cm
Gel capacity2 precast or handcast gels
Weight1.8 lbs
Recommended power supplyAzure Aqua Power Supply 600V

 

 

Item#:
ASAZAQUATRANCELL

Azure Western Blot Quenching Sheets

 

Background quenching sheets for use with fluorescent or chemiluminescent samples

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.

  • For use with fluorescent or chemiluminescent samples.
  • Size: 9 x 12.5 cm
  • Quantity: 10 Sheets

Features

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.

The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.




Item#:
ASAZQUENCH

Azure Aqua Quad Mini-Cell

 

Vertical Gel Running System

Designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis. The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels.

Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize. Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

 

Includes:

  • 4 gel casting stands and casting frames buffer dam
  • 5 spacer plates (1mm)
  • 10 short glass plates
  • 5 15-well combs
  • 5 10-well combs
  • 5 sealing gaskets
  • 5 gel shovels
  • Tank and lid with power cables
  • Primary and Secondary Electrode Cores

 

Features

  • The Azure Aqua Quad Mini-Cell is designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis.
  • The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels: Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize.
  • Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

 


Specifications
 
ParametersAzure Aqua Quad Mini-Cell Specs
Unit dimensions (W x L x H)13.5 cm x 17.5 cm x 19 cm
Weight2.2 lbs
Recommended power supplyAzure Aqua Power Supply 600V
Number of gels1-4 precast (most major brands) or handcast gels
Tank buffer volume2 gels: 540 mL | 4 gels: 1100 mL
Maximum gel size6.7 cm x 8.6 cm
Typical run times30-45 minutes at 200V
Gel cassette size (WxL)10 cm x 8 cm

 

Item#:
ASAZAQUADMINI

Azure Flash Western Chemiluminescence Kit

 

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

 

Each kit includes:

  • 10x PVDF Membranes
  • 100mL Chemi Blot Blocking Buffer
  • 500μL Flash HRP Conjugate
  • 250mL Flash Wash Buffer
  • 100mL Radiance ECL Substrate

Features

 

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

 

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

  • Fast protocol with few hands-on steps
  • Antibody stabilization for enhanced chemi signal
  • Low background and increased signal-to-noise ratios
  • Increased detection sensitivity and linear dynamic range

 

 


Hoefer® Gradient Makers

 

Ideal for generating gradients of polyacrylamide, sucrose, and cesium chloride.

  • Made of heavy acrylic plastic with high-quality, leak-free Teflon® valves
  • Flat base provides stability on the magnetic stir. Comes with a support rod for attaching to a ring stand
  • Takes Luer or 4 mm ID tubing. With included adapters, it can also take 2 mm ID or 22-gauge tubing

SG15, SG30, SG50 and SG100 include

  • Gradient Maker with Teflon® Valves, 1/ea
  • Adapter Barbed Fitting, 1/ea
  • Luer Outlet Fitting, 1/ea
  • 22-gauge Needle, 1/ea
  • Support Rod, 1/ea 

The 500 mL SG500 Gradient Maker is made of two cylindrical acrylic chambers joined and mounted on a flat acrylic base.

  • An easy-to-use push-pull valve opens and closes the passage between the reservoir and mixing chambers
  • Suitable for forming polyacrylamide gradients or buffer gradients used with medium to small chromatography columns
  • Ideal for use with the SE615 Multiple Gel Caster  

SG500 includes

  • Gradient Maker with Push-Pull Valve, 1/ea
  • Hose Clamps, 4/pk

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASHOFRGRADMKRS


Hoefer® Gel Casters

 

Enable several gradient or homogeneous gels to be cast simultaneously. Choice of multiple-gel casters or dual-gel casters.

 

Gel Casters for Small Format Vertical Electrophoresis Systems (SE250, SE260, SE300)

 

Multiple-Gel Casters

  • Pour uniform gels from the top; pump gradient gels into an inlet port at the bottom of the unit
  • Sandwiches fill at the same time and at the same rate from the same solution
  • The result: identical gels
  • Glass plate sandwiches seal leak-free without the use of messy grease or tape
  • Once cast, gels can be wrapped in plastic and refrigerated for several weeks

Dual-Gel Caster (SE245)

  • Cast one or two gels
  • Accommodates both 10 x 8 cm and 10 x 10.5 cm plates

*For use with the SE250 and SE260 Series only.
 

Gel Casters for SE600 and SE400 Series Vertical Units   

 

Multiple-Gel Casters

  • SE675 casts up to four 16 cm long gels
  • SE615 casts up to ten 16 cm long gels
  • Easy to use and leak-free
  • Cast homogeneous or gradient gels. Pour homogeneous gels directly into the top of the casting chamber: pump gradient gels through an inlet port at the bottom

Dual-Gel Caster (SE6015)

 

SE6015 Dual Gel Caster is included when you order an SE600 series unit. It can also be ordered separately.

  • Casts two 8, 16, or 24 cm long gels at a time
  • Double the capacity with optional divider plates
  • Easy to use and leak-free
  • Glass Sandwichers seal into the casting 

*For use with the SE600 Series only.

 

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASHOFRGLCSTRS


Hoefer® Combs for SUB15 and SUBHT Submarine Gel Electrophoresis Systems

 

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer®Â combs ensure sharp, consistent, well formation for accurate, reproducible results.

 

MC = Microtiter spacing for use with a multichannel pipette
Sample volume listed is for a 5 mm deep well

For use with SUB15 and SUBHT Submarine Gel Electrophoresis Systems

 

Specifications
 
Item #DescriptionWellsTeethThicknessWidthSample Vol.
SUB15-C1-10SUB15 and SUBHT Comb10 Wells10 Teeth1 mm12.8 mm64 uL
SUB15-C15-10SUB15 and SUBHT Comb10 Wells10 Teeth1.5 mm12.8 mm96 uL
SUB15-C2-10SUB15 and SUBHT Comb10 Wells10 Teeth2 mm12.8 mm128 uL
SUB15-C1-16MCSUB15 and SUBHT Comb16 Wells16 Teeth1 mm6.4 mm32 uL
SUB15-C15-16MCSUB15 and SUBHT Comb16 Wells16 Teeth1.5 mm6.4 mm48 uL
SUB15-C2-16MCSUB15 and SUBHT Comb16 Wells16 Teeth2 mm6.4 mm64 uL
SUB15-C1-20SUB15 and SUBHT Comb20 Wells20 Teeth1 mm5.9 mm29 uL
SUB15-C15-20SUB15 and SUBHT Comb20 Wells20 Teeth1.5 mm5.9 mm43 uL
SUB15-C2-20SUB15 and SUBHT Comb20 Wells20 Teeth2 mm5.9 mm58 uL
SUB15-C1-25SUB15 and SUBHT Comb25 Wells25 Teeth1 mm3.5 mm17 uL
SUB15-C15-25SUB15 and SUBHT Comb25 Wells25 Teeth1.5 mm3.5 mm25 uL
SUB15-C2-25SUB15 and SUBHT Comb25 Wells25 Teeth2 mm3.5 mm34 uL
SUB15-C1-30MCSUB15 and SUBHT Comb30 Wells30 Teeth1 mm2.5 mm12 uL
SUB15-C15-30MCSUB15 and SUBHT Comb30 Wells30 Teeth1.5 mm2.5 mm18 uL
SUB15-C2-30MCSUB15 and SUBHT Comb30 Wells30 Teeth2 mm2.5 mm24 uL

Proudly made in the USA! Click here for more made in America products!
 
 

Item#:
ASHOFRCOMBS8


Hoefer® Combs for SUB20 and SUB20C Submarine Gel Electrophoresis Systems


Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

Specifications
 
Item #DescriptionWellsTeethThicknessWidthSample Vol.
SUB20-C1-16SUB20 and SUB20C Comb16 Wells16 Teeth1 mm8.5 mm35 uL
SUB20-C15-16SUB20 and SUB20C Comb16 Wells16 Teeth1.5 mm8.5 mm55 uL
SUB20-C2-16SUB20 and SUB20C Comb16 Wells16 Teeth2 mm8.5 mm75 uL
SUB20-C1-20MCSUB20 and SUB20C Comb20 Wells20 Teeth1 mm7 mm30 uL
SUB20-C15-20MCSUB20 and SUB20C Comb20 Wells20 Teeth1.5 mm7 mm45 uL
SUB20-C2-20MCSUB20 and SUB20C Comb20 Wells20 Teeth2 mm7 mm60 uL
SUB20-C1-28SUB20 and SUB20C Comb28 Wells28 Teeth1 mm5 mm20 uL
SUB20-C15-28SUB20 and SUB20C Comb28 Wells28 Teeth1.5 mm5 mm30 uL
SUB20-C2-28SUB20 and SUB20C Comb28 Wells28 Teeth2 mm5 mm40 uL
SUB20-C1-40MCSUB20 and SUB20C Comb40 Wells40 Teeth1 mm3 mm13 uL
SUB20-C15-40MCSUB20 and SUB20C Comb40 Wells40 Teeth1.5 mm3 mm19 uL
SUB20-C2-40MCSUB20 and SUB20C Comb40 Wells40 Teeth2 mm3 mm25 uL
 

Proudly made in the USA! Click here for more made in America products!
 
 

Item#:
ASHOFRCOMBS9


Hoefer® Combs for SUB10 Submarine Gel Electrophoresis Systems

 

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer®Â combs ensure sharp, consistent, well formation for accurate, reproducible results.

Specifications

Item #DescriptionWellsTeethThicknessWidthSample Vol.
SUB10-C1-8SUB10 Comb8 Wells8 Teeth1 mm9 mm40 uL
SUB10-C15-8SUB10 Comb8 Wells8 Teeth1.5 mm9 mm60 uL
SUB10-C2-8SUB10 Comb8 Wells8 Teeth2 mm9 mm80 uL
SUB10-C1-10MCSUB10 Comb10 Wells10 Teeth1 mm7 mm30 uL
SUB10-C15-10MCSUB10 Comb10 Wells10 Teeth1.5 mm7 mm45 uL
SUB10-C2-10MCSUB10 Comb10 Wells10 Teeth2 mm7 mm60 uL
SUB10-C1-12SUB10 Comb12 Wells12 Teeth1 mm5.5 mm25 uL
SUB10-C15-12SUB10 Comb12 Wells12 Teeth1.5 mm5.5 mm35 uL
SUB10-C2-12SUB10 Comb12 Wells12 Teeth2 mm5.5 mm50 uL
SUB10-C1-16SUB10 Comb16 Wells16 Teeth1 mm3.6 mm15 uL
SUB10-C15-16SUB10 Comb16 Wells16 Teeth1.5 mm3.6 mm25 uL
SUB10-C2-16SUB10 Comb16 Wells16 Teeth2 mm3.6 mm30 uL
SUB10-C1-20MCSUB10 Comb20 Wells20 Teeth1 mm3 mm12 uL
SUB10-C15-20MCSUB10 Comb20 Wells20 Teeth1.5 mm3 mm20 uL
SUB10-C2-20MCSUB10 Comb20 Wells20 Teeth2 mm3 mm25 uL 


Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASHOFRCOMBS6
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