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PR1MA™ Rapid Coomassie Stain
- Appearance: Dark Blue Color
- Concentration: 20X
- Solubility: Suitable for use in staining protein in polyacrylamide gels
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Rapid, Commassie, Stain, PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
PR1MA™ Protein Gel-Loading Dye for SDS-PAGE
- Appearance: Slightly Viscous Dark Purple Liquid
- DNase, RNase, protease: Not Detected
- Electrophoresis: Pass Test
- OPTICAL ABS AT 525 NM: 0.15 - 0.35
- OPTICAL ABS BETWEEN 588-594 NM: 0.32 - 0.47
- OPTICAL ABS BETWEEN 635-641 NM: 0.25 - 0.40
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Protein, Gel-Loading, Dye, SDS-PAGE,PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
Gel Crusher™ Spin Column
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Rapid Gel Fragmentation for DNA Recovery.
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Gel Crusher™ Spin Columns provide a fast, efficient, and consistent method for gel fragmentation, replacing traditional crush-and-soak or manual gel breaker steps. Designed with 600 uM filtration pores, these columns enable quick and uniform fragmentation of agarose or acrylamide gel slices, saving time and increasing DNA recovery yields.
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Ideal for workflows in molecular cloning, PCR cleanup, restriction digests, DNA sequencing, and labeling protocols, the Gel Crusher™Â simplifies the preparation of gel fragments for downstream purification and elution steps.
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Simply insert the Gel Crusher™Â column into a 2 mL microcentrifuge tube, add your pre-cut gel slice (prepared using a gel cutter or razor blade), and centrifuge at max speed (~15,000 x g) for 5 minutes. The gel will be forced through the 600 uM pores, producing small, uniform fragments that can be easily pipetted using wide-bore tips into NaCl or other solutions.
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This process creates a fine slurry that enhances elution efficiency and improves access to the target nucleic acids, significantly reducing incubation and processing time.
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Key Features
- Efficiently breaks gel slices into uniform fragments
- Eliminates manual crush-and-soak steps
- Increases DNA yield and recovery efficiency
- Fits standard 2 mL microcentrifuge tubes
- Compatible with standard benchtop centrifuges (up to 15,000 x g)
- 600 uM pore size ideal for agarose or acrylamide gels
- Simplifies downstream processes: elution, filtration, incubation
- For use in PCR, cloning, digestion, sequencing, and moreÂ
Reversible Racks
- Choose 60 well (5 x 12) or 96 well (8 x 12) formats
- Reversible racks, accept 0.5 and 1.5 to 2.0 mL microtubes
- Alphanumeric marking on each well
- Made of polypropylene
Azure Aqua Transfer Cell
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Perform a quick 1 hour transfer or transfer overnight at a lower voltage
Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.
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Includes:
- 2 transfer cassettes
- 6 sponges
- 2 Azure Aqua cooling units
- Tank and upper lid with power cables
- Transfer Electrode Cores
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Features
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The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.
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Chemical reagent compatibility
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Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.
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Cleaning Instructions
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Before using, wash all components with a neutral detergent and rinse well with ultra pure water.
Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.
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Specifications
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| Parameters | Azure Aqua Transfer Cell Specs |
| Number of cassettes | 2 |
| Buffer requirement | 1100 mL |
| Electrode distance | 4 cm |
| Electrode assembly size ( L x W x H) | 17.5 cm x 13.5 cm x 19 cm |
| Transfer time | 60 minutes |
| Cooling | Azure Aqua cooling unit |
| Blotting area | 7.5 cm x 10 cm |
| Buffer volume (with cooling unit) | 650 mL |
| Buffer volume (without cooling unit) | 850 mL |
| Unit dimensions (W x L x H) | 13.5 cm x 17.5 cm x 19 cm |
| Tank buffer volume | 1100 mL |
| Maximum gel size | 7.5 cm x 10 cm |
| Gel capacity | 2 precast or handcast gels |
| Weight | 1.8 lbs |
| Recommended power supply | Azure Aqua Power Supply 600V |
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Azure Western Blot Quenching Sheets
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Background quenching sheets for use with fluorescent or chemiluminescent samples
Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.
- For use with fluorescent or chemiluminescent samples.
- Size: 9 x 12.5 cm
- Quantity: 10 Sheets
Features
Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.
The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.
Azure Aqua Quad Mini-Cell
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Vertical Gel Running System
Designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis. The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels.
Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize. Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.
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Includes:
- 4 gel casting stands and casting frames buffer dam
- 5 spacer plates (1mm)
- 10 short glass plates
- 5 15-well combs
- 5 10-well combs
- 5 sealing gaskets
- 5 gel shovels
- Tank and lid with power cables
- Primary and Secondary Electrode Cores
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Features
- The Azure Aqua Quad Mini-Cell is designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis.
- The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels: Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize.
- Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.
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Specifications
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| Parameters | Azure Aqua Quad Mini-Cell Specs |
| Unit dimensions (W x L x H) | 13.5 cm x 17.5 cm x 19 cm |
| Weight | 2.2 lbs |
| Recommended power supply | Azure Aqua Power Supply 600V |
| Number of gels | 1-4 precast (most major brands) or handcast gels |
| Tank buffer volume | 2 gels: 540 mL | 4 gels: 1100 mL |
| Maximum gel size | 6.7 cm x 8.6 cm |
| Typical run times | 30-45 minutes at 200V |
| Gel cassette size (WxL) | 10 cm x 8 cm |
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Azure Flash Western Chemiluminescence Kit
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Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol
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Each kit includes:
- 10x PVDF Membranes
- 100mL Chemi Blot Blocking Buffer
- 500μL Flash HRP Conjugate
- 250mL Flash Wash Buffer
- 100mL Radiance ECL Substrate
Features
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Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.
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The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.
- Fast protocol with few hands-on steps
- Antibody stabilization for enhanced chemi signal
- Low background and increased signal-to-noise ratios
- Increased detection sensitivity and linear dynamic range
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Hoefer® Gradient Makers
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Ideal for generating gradients of polyacrylamide, sucrose, and cesium chloride.
- Made of heavy acrylic plastic with high-quality, leak-free Teflon® valves
- Flat base provides stability on the magnetic stir. Comes with a support rod for attaching to a ring stand
- Takes Luer or 4 mm ID tubing. With included adapters, it can also take 2 mm ID or 22-gauge tubing
SG15, SG30, SG50 and SG100 include
- Gradient Maker with Teflon® Valves, 1/ea
- Adapter Barbed Fitting, 1/ea
- Luer Outlet Fitting, 1/ea
- 22-gauge Needle, 1/ea
- Support Rod, 1/eaÂ
The 500 mL SG500 Gradient Maker is made of two cylindrical acrylic chambers joined and mounted on a flat acrylic base.
- An easy-to-use push-pull valve opens and closes the passage between the reservoir and mixing chambers
- Suitable for forming polyacrylamide gradients or buffer gradients used with medium to small chromatography columns
- Ideal for use with the SE615 Multiple Gel Caster Â
SG500 includes
- Gradient Maker with Push-Pull Valve, 1/ea
- Hose Clamps, 4/pk
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Hoefer® Gel Casters
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Enable several gradient or homogeneous gels to be cast simultaneously. Choice of multiple-gel casters or dual-gel casters.
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Gel Casters for Small Format Vertical Electrophoresis Systems (SE250, SE260, SE300)
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Multiple-Gel Casters
- Pour uniform gels from the top; pump gradient gels into an inlet port at the bottom of the unit
- Sandwiches fill at the same time and at the same rate from the same solution
- The result: identical gels
- Glass plate sandwiches seal leak-free without the use of messy grease or tape
- Once cast, gels can be wrapped in plastic and refrigerated for several weeks
Dual-Gel Caster (SE245)
- Cast one or two gels
- Accommodates both 10 x 8 cm and 10 x 10.5 cm plates
*For use with the SE250 and SE260 Series only.
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Gel Casters for SE600 and SE400 Series Vertical Units Â
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Multiple-Gel Casters
- SE675 casts up to four 16 cm long gels
- SE615 casts up to ten 16 cm long gels
- Easy to use and leak-free
- Cast homogeneous or gradient gels. Pour homogeneous gels directly into the top of the casting chamber: pump gradient gels through an inlet port at the bottom
Dual-Gel Caster (SE6015)
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SE6015 Dual Gel Caster is included when you order an SE600 series unit. It can also be ordered separately.
- Casts two 8, 16, or 24 cm long gels at a time
- Double the capacity with optional divider plates
- Easy to use and leak-free
- Glass Sandwichers seal into the castingÂ
*For use with the SE600 Series only.
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Hoefer® Combs for SUB15 and SUBHT Submarine Gel Electrophoresis Systems
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Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer®Â combs ensure sharp, consistent, well formation for accurate, reproducible results.
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MC = Microtiter spacing for use with a multichannel pipette
Sample volume listed is for a 5 mm deep well
For use with SUB15 and SUBHT Submarine Gel Electrophoresis Systems
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Specifications
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| Item # | Description | Wells | Teeth | Thickness | Width | Sample Vol. |
| SUB15-C1-10 | SUB15 and SUBHT Comb | 10 Wells | 10 Teeth | 1 mm | 12.8 mm | 64 uL |
| SUB15-C15-10 | SUB15 and SUBHT Comb | 10 Wells | 10 Teeth | 1.5 mm | 12.8 mm | 96 uL |
| SUB15-C2-10 | SUB15 and SUBHT Comb | 10 Wells | 10 Teeth | 2 mm | 12.8 mm | 128 uL |
| SUB15-C1-16MC | SUB15 and SUBHT Comb | 16 Wells | 16 Teeth | 1 mm | 6.4 mm | 32 uL |
| SUB15-C15-16MC | SUB15 and SUBHT Comb | 16 Wells | 16 Teeth | 1.5 mm | 6.4 mm | 48 uL |
| SUB15-C2-16MC | SUB15 and SUBHT Comb | 16 Wells | 16 Teeth | 2 mm | 6.4 mm | 64 uL |
| SUB15-C1-20 | SUB15 and SUBHT Comb | 20 Wells | 20 Teeth | 1 mm | 5.9 mm | 29 uL |
| SUB15-C15-20 | SUB15 and SUBHT Comb | 20 Wells | 20 Teeth | 1.5 mm | 5.9 mm | 43 uL |
| SUB15-C2-20 | SUB15 and SUBHT Comb | 20 Wells | 20 Teeth | 2 mm | 5.9 mm | 58 uL |
| SUB15-C1-25 | SUB15 and SUBHT Comb | 25 Wells | 25 Teeth | 1 mm | 3.5 mm | 17 uL |
| SUB15-C15-25 | SUB15 and SUBHT Comb | 25 Wells | 25 Teeth | 1.5 mm | 3.5 mm | 25 uL |
| SUB15-C2-25 | SUB15 and SUBHT Comb | 25 Wells | 25 Teeth | 2 mm | 3.5 mm | 34 uL |
| SUB15-C1-30MC | SUB15 and SUBHT Comb | 30 Wells | 30 Teeth | 1 mm | 2.5 mm | 12 uL |
| SUB15-C15-30MC | SUB15 and SUBHT Comb | 30 Wells | 30 Teeth | 1.5 mm | 2.5 mm | 18 uL |
| SUB15-C2-30MC | SUB15 and SUBHT Comb | 30 Wells | 30 Teeth | 2 mm | 2.5 mm | 24 uL |
Proudly made in the USA! Click here for more made in America products!
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Hoefer® Combs for SUB20 and SUB20C Submarine Gel Electrophoresis Systems
Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.
- MC = Microtiter spacing for use with a multichannel pipette
- Sample volume listed is for a 5 mm deep well
- For use with SUB20 and SUB20C Submarine Gel Electrophoresis Systems
Specifications
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| Item # | Description | Wells | Teeth | Thickness | Width | Sample Vol. |
| SUB20-C1-16 | SUB20 and SUB20C Comb | 16 Wells | 16 Teeth | 1 mm | 8.5 mm | 35 uL |
| SUB20-C15-16 | SUB20 and SUB20C Comb | 16 Wells | 16 Teeth | 1.5 mm | 8.5 mm | 55 uL |
| SUB20-C2-16 | SUB20 and SUB20C Comb | 16 Wells | 16 Teeth | 2 mm | 8.5 mm | 75 uL |
| SUB20-C1-20MC | SUB20 and SUB20C Comb | 20 Wells | 20 Teeth | 1 mm | 7 mm | 30 uL |
| SUB20-C15-20MC | SUB20 and SUB20C Comb | 20 Wells | 20 Teeth | 1.5 mm | 7 mm | 45 uL |
| SUB20-C2-20MC | SUB20 and SUB20C Comb | 20 Wells | 20 Teeth | 2 mm | 7 mm | 60 uL |
| SUB20-C1-28 | SUB20 and SUB20C Comb | 28 Wells | 28 Teeth | 1 mm | 5 mm | 20 uL |
| SUB20-C15-28 | SUB20 and SUB20C Comb | 28 Wells | 28 Teeth | 1.5 mm | 5 mm | 30 uL |
| SUB20-C2-28 | SUB20 and SUB20C Comb | 28 Wells | 28 Teeth | 2 mm | 5 mm | 40 uL |
| SUB20-C1-40MC | SUB20 and SUB20C Comb | 40 Wells | 40 Teeth | 1 mm | 3 mm | 13 uL |
| SUB20-C15-40MC | SUB20 and SUB20C Comb | 40 Wells | 40 Teeth | 1.5 mm | 3 mm | 19 uL |
| SUB20-C2-40MC | SUB20 and SUB20C Comb | 40 Wells | 40 Teeth | 2 mm | 3 mm | 25 uL |
Proudly made in the USA! Click here for more made in America products!
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Hoefer® Combs for SUB10 Submarine Gel Electrophoresis Systems
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Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer®Â combs ensure sharp, consistent, well formation for accurate, reproducible results.
- MC = Microtiter spacing for use with a multichannel pipette
- Sample volume listed is for a 5 mm deep well
- For use with SUB10 Submarine Gel Electrophoresis Systems
Specifications
| Item # | Description | Wells | Teeth | Thickness | Width | Sample Vol. |
| SUB10-C1-8 | SUB10 Comb | 8 Wells | 8 Teeth | 1 mm | 9 mm | 40 uL |
| SUB10-C15-8 | SUB10 Comb | 8 Wells | 8 Teeth | 1.5 mm | 9 mm | 60 uL |
| SUB10-C2-8 | SUB10 Comb | 8 Wells | 8 Teeth | 2 mm | 9 mm | 80 uL |
| SUB10-C1-10MC | SUB10 Comb | 10 Wells | 10 Teeth | 1 mm | 7 mm | 30 uL |
| SUB10-C15-10MC | SUB10 Comb | 10 Wells | 10 Teeth | 1.5 mm | 7 mm | 45 uL |
| SUB10-C2-10MC | SUB10 Comb | 10 Wells | 10 Teeth | 2 mm | 7 mm | 60 uL |
| SUB10-C1-12 | SUB10 Comb | 12 Wells | 12 Teeth | 1 mm | 5.5 mm | 25 uL |
| SUB10-C15-12 | SUB10 Comb | 12 Wells | 12 Teeth | 1.5 mm | 5.5 mm | 35 uL |
| SUB10-C2-12 | SUB10 Comb | 12 Wells | 12 Teeth | 2 mm | 5.5 mm | 50 uL |
| SUB10-C1-16 | SUB10 Comb | 16 Wells | 16 Teeth | 1 mm | 3.6 mm | 15 uL |
| SUB10-C15-16 | SUB10 Comb | 16 Wells | 16 Teeth | 1.5 mm | 3.6 mm | 25 uL |
| SUB10-C2-16 | SUB10 Comb | 16 Wells | 16 Teeth | 2 mm | 3.6 mm | 30 uL |
| SUB10-C1-20MC | SUB10 Comb | 20 Wells | 20 Teeth | 1 mm | 3 mm | 12 uL |
| SUB10-C15-20MC | SUB10 Comb | 20 Wells | 20 Teeth | 1.5 mm | 3 mm | 20 uL |
| SUB10-C2-20MC | SUB10 Comb | 20 Wells | 20 Teeth | 2 mm | 3 mm | 25 uLÂ |