hydroGrade - Hydrophilic Surface

BRANDplates® hydroGrade plates feature a strongly hydrophilic surface, with a strong affinity to hydrophilic molecules, such as glycoproteins and peptides, antibodies with predominantly hydrophilic regions, and nucleic acids. They are an alternative to the immunoG plates for homogeneous assays with hydrophobic molecules that remain in solution.

• hydroGrade assay plates are offered in 96 and 384 well formats; 96-well plates are available in different well geometries
• Treated to bind biomolecules with predominatly hydrophilic regions
• Surface choice to bind glycoproteins, nucleic acids and other hydrophilic molecules, or for homogeneous assays with hydrophobic molecules that remain in solution
• Polystyrene, Non-sterile
• Colors available include transparent, white and black
• Blue alphanumeric coding on 96 well plates
• Free from endotoxins, DNase, DNA, RNase
• Standard plates are packaged in 20 stacks of 5 plates
• Each inner pack contains one lid (i.e. one lid per 5 plates for standard plates; one lid per 25 plates for transparent bottom plates)
• Lids available separately

At a glance:  Microplates for immunoassays:

• Standard plate for the cultivation of adherent cell lines
• Hydrophilic surface with freely accessible carboxyl and hydroxyl groups
• Serum components are easily bound, allowing cells to directly attche and spread out quickly with a better rate of yield
• Transparent bottom and low-volume versions available
• DNase/RNase, pyrogen-free
• Sterile
  
  At a glance: BRANDplates® for cell culture:

lipoGrade™ - Hydrophobic Surface

lipoGrade™ plates feature a strongly hydrophobic/lipophilic surface, for the immobilization of biomolecules with strongly hydrophobic regions. They are an alternative to the immunoGrade™ plates for solid phase assays, and are a choice for homogeneous assays with hydrophilic molecules that remain in solution.

• lipoGrade™ assay plates are offered in 96 and 384 well formats; 96-well plates are available in different well geometries
• Treated to bind biomolecules with predominantly hydrophobic regions
• Surface choice to bind lipoproteins, peptides, and other hydrophobic molecules, or for homogeneous assays with hydrophilic molecules that remain in solution
• Non-sterile, polystyrene
• Colors available include transparent, white and black, along with colored plates with transparent bottoms
• Blue alphanumeric coding on 96 well plates
• Free from endotoxins, DNase, DNA, RNase
• Packaged in 10 stacks of 5 plates
• Lids available separately

CellGrade™ for the cultivation of adherent cell lines

• Standard plate for the cultivation of adherent cell lines.
• PS-surface with different chemical groups, such as carboxyl and hydroxyl groups, that are freely accessible.
• Surface is hydrophilic compared with non-treated PS.
• Serum components are easily bound onto the freely accessible chemical groups, allowing an indirect adhesion of cells.
• Clearly distinguishable through the color code: orange, embossed alphanumeric coding for 96-well standard plates.
• Free from endotoxins, DNase, DNA, RNase, non-cytotoxic.
• PS, sterile, with lids.
• BIO-CERT^® CELL CULTURE QUALITY: Free of human DNA, RNase, DNase, Pyrogens and non-cytotoxic

Specifications:

• Poly-D-Lysine equivalent surface, with analogous results regarding growth performance and cell morphology
• Optimal adhesion of cells to the surface reduces cell damage when washing frequently
• Cultivation of cell lines with the highest demands on their environment
• Surface suited for serum-free and serum-reduced cell cultivation
• Alternative option to biologically coated surfaces
• Longer shelf life at room temp than biologically coated surfaces
• DNase/RNase, pyrogen-free
• Sterile

At a glance: BRANDplates® for cell culture:

All BRANDplates cell culture plates are sterilized by Ã-radiation to ISO 11137 and AAMI guidelines for a SAL of 10-6, meeting the requirements of Ph. Eur. and the USP 29. Plates are DNA-, DNase-, RNase-free, as well as meeting cytotoxic substance levels per DIN EN ISO 10993. Endotoxins are <0.01 EU/mL. All sterile plates are individually wrapped.

• For the cultivation of fastidious cell lines
• Surface has a protein-like composition so cells can directly attach and spread out
• Cells adhere faster, better rate of yield
• Sensitive cell lines can be cultivated
• DNase/RNase, pyrogen-free
• Sterile

At a glance: BRANDplates® for cell culture:

Plate, 96 Round Well V-Bottom 14.40mm Height, 480ul

96-well white frame tissue culture-treated (TC-treated) microplate with clear wells for radiometric and luminescence assays. The clear bottom enables bottom-reading measurements and microscopic visualization of cells.

384, Square, Well

Protran nitrocellulose membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate, which will lower the protein binding capacity. In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantees superior signal-to-noise ratios, without the need for stringent washing conditions. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins that prefer aqueous environments. Prior to transfer, the membrane is simply wet in water and then in the transfer buffer, with no other necessary pre-treatment steps. Protran membranes exhibit the best handling strangth of all pure nitrocellulose membranes.
Features:

• High binding capacity (80-150g/cm2) of proteins and nucleic acids.
• 100% pure nitrocellulose, with no cellulose acetate added, ensures the highest binding capacity possible.
• Compatible with virtually all standard detection methods.
• Low background - NC surface properties guarantee superior signal-to-noise ratios.
• Triton-free matrix with low extractable levels will support the cell growth essential for colony blotting, plaque lifts, and tissue culture applications.
• Available in many sizes and formats to fit different transfer devices and gel chambers.