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  • Excellent all-purpose amplification enzyme
  • Thermostable recombinant DNA polymerase from Thermus aquaticus
  • Exhibits very high activity in primer extension
  • Has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity
  • Does not have 3' to 5' exonuclease activity-no proofreading ability
  • Leaves an A-overhang, which makes the enzyme ideal for TA cloning
  • Includes MgCl² in buffer
Item#:
ASPCRREAG1

Taq DNA Polymerase, 2.0 Mix

2.0 Master Mix Kit (1.5mM MgCl2)

Cat. No.

Reactions

Taq DNA Polymerase

MgCl2

BE140301

100

2.0x Master Mix

1.5 mM

BE140303

500

2.0x Master Mix

1.5 mM

BE140306

2,500

2.0x Master Mix

1.5 mM

Store at -20°CFor in vitro laboratory use only

General Description

Bullseye Taq DNA Polymerase Mix is a ready-to-use 2.0X reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.

Bullseye Taq DNA Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are present in Taq DNA Polymerase Mix. Each reaction requires 25 µL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 µL.

Taq DNA Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.

    Composition of 2.0X Taq Master Mix

  • 150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3.0 mM MgCl2, 0.2% Tween 20
  • 0.4 mM dNTPs
  • 0.05 units/µL Bullseye Taq polymerase
  • Stabilizer

    Suggested Protocol using Taq Master Mix

This protocol serves as a guideline for primer extensions. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.

    Notes:

  • Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis.
  • The table below shows the reaction set up for a final volume of 50µL. If desired, the reaction size may be scaled down. Use 10 µL of the 2.0X master mix in a final volume of 20µL.
  • Important: Spin Taq Master Mix vials briefly before use.

    1. Set up each reaction as follows:

Component

Vol./reaction

Final Conc.

Taq Master Mix

25 µL

1X

Primer A

Variable

0.1.1.0µM

Primer B

Variable

0.1.1.0µM

Distilled Water

Variable

- - - -

Template DNA

Variable

Variable

TOTAL volume

50 µL

- - - -

2. Mix gently by pipetting the solution up and down a few times.

3. Program the thermal cycler according to the manufacturer's instructions. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

4. Place the tubes in the thermal cycler and start the reaction.

Item#:
ASPCRREAG3

Accuris™ Hot Start Taq

  • Exceptional sensitivity for low copy PCR
  • Ideal for multiplex PCR and amplification of GC-rich DNA
  • Same enhanced features as Accuris Taq Polymerase
  • Buffer optimized for fast cycling and reproducibility

Engineered for controlled polymerase activity, Accuris Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.

When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard Accuris Taq, Hot Start Taq is provided with a 5X buffer, or in a ready to use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye.  The Red Dye Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

 

Item No.

Description

Volume

PR1000-HS-S

Accuris Hot Start Taq - Sample

50 units

PR1000-HS-500

Accuris Hot Start Taq DNA Polymerase

500u (5u/p1)

PR1000-HS-1000

Accuris Hot Start Taq DNA Polymerase

1000u (5u/p1)

PR1000-HS-6000

Accuris Hot Start Taq DNA Polymerase

6000u (5u/p1)

PR1001-HS-S

Accuris Hot Start Taq Mastermix - Sample

5 reactions

PR1001-HS-200

Accuris Hot Start 2X Taq Master Mix

200 x 50p1 Reactions

PR1001-HS-1000

Accuris Hot Start 2X Taq Master Mix

1000 x 50p1 Reactions

PR1001-HSR-S

Accuris Hot Start Taq Master Mix Red Dye, 2X conc. - Sample

5 reactions

PR1001-HSR-200

Accuris Hot Start 2X Taq Red Master Mix

200 x 50p1 Reactions

PR1001-HSR-1000

Accuris Hot Start 2X Taq Red Master Mix

1000 x 50p1 Reactions

Item#:
ASACCREAG2

Accuris™ High Fidelity Polymerase

  • 50X higher fidelity than Taq DNA polymerase
  • Works with crude DNA sample
  • Ideal for cloning, mutagenesis and microarrays
  • Produces blunt end products, to clone directly into blunt end vectors
  • Optimized buffer system with unique PCR enhancers

For applications requiring highly accurate amplification, choose Accuris High Fidelity DNA polymerase. Modified for better solubility and higher activity across a broad range of ionic conditions, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples.

A 3'-5' proofreading exonuclease activity and an error rate of 4.55×10-7 makes Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets.

 

Item No.

Description

Volume

PR1000-HF-S

Accuris High Fidelity DNA Polymerase - Sample

20 units

PR1000-HF-200

Accuris High Fidelity DNA Polymerase

200u (2u/p1)

PR1000-HF-1000

Accuris High Fidelity DNA Polymerase

1000u (2u/p1)

Item#:
ASACCREAG4

Accuris High Fidelity Hot Start Master Mix
  • Leaves an A-overhang for TA cloning
  • Ideal for difficult, high GC content sequences
  • 10x fidelity of native Taq
  • Ideal for long PCR, up to 10kb targets

Accuris High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, an antibody mediated hot start mechanism, and a proof-reading component for trouble-free PCR reaction assembly and performance.

The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.

Item#:
ASACCURISHIFIDHS

Accuris High Fidelity Master Mix

  • Leaves a blunt end
  • Rapid extension: up to 1 kb per 15 seconds
  • 100x fidelity of native Taq
  • Ideal for shorter, less complex targets

A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, Accuris High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.

The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.

Item#:
ASACCURISHIFIDMM

Accuris™ Mammalian Genotyping Kit

  • DNA extraction and amplification in 1 hour.
  • Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues.
  • Single tube - no organic solvents or clean up procedures.
  • Enhanced Accuris Hot Start Polymerase included.

Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.

The Accuris Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR Accuris Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers.

Item No.

Description

Volume

PR1300-MG-S

Accuris 1 Hour Mammalian Genotyping Kit - Sample

8 reactions

PR1300-MG-80

Accuris 1 Hour Mammalian Genotyping kit

80

PR1300-MG-400

Accuris 1 Hour Mammalian Genotyping kit

400

Item#:
ASACCREAG5

Accuris™ Taq

  • Ideal for routine PCR applications as well as genotyping, colony PCR and fast PCR
  • Improved template affinity and solubility for higher enzyme activity and greater yields
  • Proprietary buffer optimized for a variety of assay conditions
  • Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer

Accuris Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. Accuris Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.

The polymerase is supplied with a 5X buffer containing MgCl2 and proprietary mix of enhancers (dNTPs not included). For convenience, Accuris Taq is also available in a ready to use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

 

Item No.

Description

Size

PR1000-S

Accuris Taq Polymerase - Sample

50 units

PR1000-500

AccurisTaq DNA Polymerase

500 u (5 u/µL)

PR1000-1000

Accuris Taq DNA Polymerase

1000 u (5 u/µL)

PR1000-6000

Accuris Taq DNA Polymerase

6000 u (5 u/µL)

PR1001-S

Accuris Taq Master Mix, 2X conc. - Sample

5 Reactions

PR1001-200

Accuris 2xTaq Master Mix

200 x 50 µL Reactions

PR1001-1000

Accuris 2x Taq Master Mix

1000 x 50 µL Reactions

PR1001-R-S

Accuris Taq Master Mix Red Dye, 2X conc. - Sample

5 Reactions

PR1001-R-200

Accuris 2x Taq Red Dye Master Mix

200 x 50 µL Reactions

PR1001-R-1000

Accuris 2x Taq Red Dye Master Mix

1000 x 50 µL Reactions

Item#:
ASACCREAG1

Accuris™ Taq Plus

  • Higher fidelity for long PCR amplicons, up to 35kb
  • Ideal for problematic templates
  • Better sensitivity and higher activity for low copy and long PCR
  • Enzyme of choice for TA cloning

Accuris Taq Plus, an optimized blend of our Hot Start Taq and a proofreading polymerase, provides 5x better fidelity than wild-type Taq and increased enzyme activity. It is the perfect choice for GC and AT rich templates, low copy number and long PCR.
The 3' -5' exonuclease activity of the proofreading enzyme produces fragments suitable for TA cloning. Accuris Taq Plus is provided with a 5X buffer with dNTPs or in a convenient one tube 2X Master Mix.

 

Item No.

Description

Volume

PR1000-TP-S

Accuris Taq Plus - Sample

50 units

PR1000-TP-250

Accuris Taq Start Taq DNA Polymerase

250u (5u/p1)

PR1000-TP-500

Accuris Taq Start Taq DNA Polymerase

1000u (5u/p1)

PR1001-TP-S

Accuris Taq Plus Master Mix, 2X conc. - Sample

20 reactions

PR1001-TP-200

Accuris 2X Taq Plus Master Mix

200x 50µl Reactions

PR1001-TP-1000

Accuris 2X Taq Plus Master Mix

1000x 50µl Reactions

Item#:
ASACCREAG3

Bullseye High Fidelity PCR Master Mix


The newly developed Bullseye HFL PCR Master Mix represents the highest fidelity for a PCR product. Bullseye's HFL PCR Master Mix has eight times higher fidelity when compared to Pfu, previously considered to be the golden standard for high fidelity PCR. This Master Mix also works well for large PCR fragments. 

The Bullseye HFL PCR Master Mix is in a 2X format and contains modified, high fidelity thermal stable DNA polymerases, in a pre-optimized PCR buffer. The amplified DNA will be blunt-ended. When using this master mix for most PCR experiments, only template, primers and H2O will be needed.

SPECIAL FEATURES & APPLICATIONS
  • High fidelity with robustness
  • Large fragment PCR
  • 2X master mix - makes PCR easy and consistent
  • For cloning, promoter study, RACE, DNA sequencing and more
Item#:
BE135HFL
Your Price:
88.00
Each

New and Improved! Ideal for General PCR, Genomic analysis and TA cloning!


Bulleye Taq DNA Polymerase is the most popular thermostable enzyme used in DNA amplification experiments. This high performance Taq DNA polymerase is specifically purified to produce excellent yields with little or no background. Its outstanding activity and unique thermal DNA amplification properties make it one of the best-value DNA polymerases available.

  • Robust performance
  • Leaves 3' A overhang
  • Stable at all storage temperatures
  • Ideal for general PCR, genomic analysis and TA cloning
  • This product is not recommended for work with neo-primers

Order#

Description

Quantity

Rxn

BETAQ-1000

Taq DNA Polymerase

1x200µl

1000U

BETAQ-5000

Taq DNA Polymerase

5x200µl

5000U

BETAQ-10000

Taq DNA Polymerase

10x200µl

10,000U

Storage: -20°C

Quality Control:
Taq DNA Polymerase is highly purified, free of contaminating endonucleases, exonucleases and nicking activity. For endonuclease assay, 1µg of Lamda/Hind III DNA is incubated with 20 units of enzyme in assay buffer at 75oC for 16 hours with no visible contaminating activity observed. Also, every lot is tested for its performance consistency.

Unit Definition:
One unit incorporates 10nmoles of 4 radioactive labeled dNTPs into acid-insoluble material in 30 minutes at 74°C.

Storage Buffer:

5 units/µl in 50mM Tris-HCl (pH8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.5% TritonX-100, and 0.5% NP-40.
10x Reaction Buffer 100mM KCl, 100mM Tris HCl (pH9.0), 80mM (NH4)2SO4, and 1.0% Triton X-100. (Mg++free): is optimized for use with 200µM dNTPs.

Magnesium Chloride:
25mM MgCl2: In general, 1.5mM MgCl2 is recommended; this may vary with different conditions and primer sets.

Please give us a call for a sample.

For laboratory research only. Not for clinical applications.

Item#:
ASPCRREAG12

Eco-Taq MasterMix, 2X,

1mL, 1 vial of 1000ul

Affordable price & shipped at room temperature.

 

A premixed, ready-to-use solution for efficient amplification of DNA templates by PCR.

  • 2X Eco-Taq MasterMix contains Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR, as well as an inert loading dye.
  • This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. To prepare the final PCR, only primers and template DNA need to be added.
  • The mix retains all features of Taq DNA Polymerase and can amplify DNA targets up to 5 kb (simple template).
  • The elongation velocity is 0.9~1.2kb/min (70~75°C).
  • It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'-dA overhang PCR product.

 

Features:

  • Convenient: Just add primers and template DNA
  • High yields of PCR products with minimal optimization.
  • High efficiency: saves your time by simplifying the process
  • Reproducible: lower contamination risk and pipetting error.

    Applications:

  • High-throughput PCR.
  • Routine PCR with high reproducibility
  • Generation of PCR products for TA cloning

    Contents:
    2X Taq Mix 1ml

    Store at -20°C
    For research use only

Item#:
ECO-TAQ1
Your Price:
20.90
Each
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