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Bullseye Pre-stained Protein

ladder, 10-180 kDa, 2x250ul

Bullseye Pre-stained Protein Ladder, 10-180 kDa

  • Ready to use - no boiling necessary
  • Stable at ambient temperature
  • 2 reference bands - red at ~75kDa and green at ~25kDa
  • Use 5-15µl, depending on the size of the wells
  • No freeze-thaw involved
  • Broad range ladder

Application: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.

The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The ladder is supplied in protein gel loading buffer and is ready-to-use. No need to heat, dilute, or add reducing agent before loading. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature; therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.


Contents:
0.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.

Usage:
For each application, 5-15µl is needed, depending on the size of the wells.

Quality Control:
Tested in polyacrylamide gel electrophoresis and Western Blotting.

Storage: -20 °C.

Note for image:

The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.
Item#:
BEPAR
Your Price:
162.80
Each

Affordable price & shipped at room temperature.

Supplied in gel loading buffer and ready to use.

  • The FroggaMark Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights (from 10 to 245 kDa).
  • Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
  • It is designed for promoting separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

 

Recommended Load:

  • 3 µL or 5 µL per loading for clear visualization during electrophoresis in 15-well or 10-well mini-gel, respectively.
  • 1.5-2.5 µL per well for general Western transferring.
  • Apply more for thicker (>1.5mm) or larger gel.

Contents: Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Trisphosphate, pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea and 15% (v/v) Glycerol.


Quality Control: Under suggested conditions, the FroggaMark Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.

Storage:

  • Stable for up to 2 weeks at 25°C
  • Store for up to 3 months at 4°C
  • For long term storage, store at -20°C
Item#:
FPL1
Your Price:
139.78
Each

RunBlue gels are more rigid and up to 10 times stronger than conventional precast gels resulting in more robust gels that can be handled with confidence. No tearing - an 8% gel can be thrown into the air and caught without breaking!

  • RunBlue gels are manufactured using a unique method of polymerisation that results in more uniform and more complete polymerisation of the Acrylamide when compared to conventional, chemical based methods. This results in gels that have superior batch to batch reproducibility and practically eliminates exposure to un-polymerised Acrylamide.
  • There is no comb to remove as the top of the teeth are fixed in place. This not only prevents the teeth from being accidentally torn off during comb removal but that all of the wells are uniform, with the teeth remaining vertical. This prevents sample cross contamination / inability to load desired Vol. The teeth also protrude above the lower front plate of the gel cassette preventing sample flow over into adjacent wells.
  • Runblue gels are available in two different cassette sizes providing compatibility with the most common gel tanks and apparatus. Â 10x10 cm cassettes are compatible with Invitrogen/Life Technologies equipment and any other equipment designed for this format, such as the Hoeffer and Owl tanks. Our 8x10 cm cassette is designed to fit the Biorad equipment (Mini Protean I, II and II, Tetra etc).
  • The resolving gel on RunBlue gels extends to the very bottom of the cassette which not only provides improved resolution but also there is no protruding gel that requires trimming prior to blotting. There is also no additional tape / covers to remove - simply take the gel from the pack and use.
  • RunBlue gels are supplied in boxes of 10 gels. They are available in a 12 well format, covering a range of concentrations and gradients.

 

Benefits of RunBlue Precast Gels

  • 2 year shelf life - never throw away another gel
  • Up to 10x stronger - no more torn, ruined gels
  • Load up to 20µl - see everything in your sample
  • No comb to remove - no more broken or bent teeth
  • Full length resolving gel - improved resolution and better blotting
  • 10x10 cm and 8x10 cm cassette - total compatibility with most popular tanks including Invitrogen and Biorad equipment
  • Fast delivery with immediate and expert technical support for total peace of mind
Best value gels available - save money!
Item#:
ASPRECASTGLIFE

Glow Writer is a fine-point phosphorescent ink marking pen for making experimental notations and other markings on chemiluminescent and radioisotope-labeled substrate materials (e.g., transfer membranes, dried gels, genomic and proteomic matrices, etc.) being analyzed by X-ray film autography or electronic imaging. The unique marker delivers a phosphor-containing ink through a fine point porous nib. Before exposure to X-ray film, the phosphorescent writing is excited by room light. A phosphor ink having an optimum concentration of phosphor and a relatively short afterglow halflife (approximately 2-3 minutes) has been selected so that upon exposure to X-ray film,autographic exposures of almost any duration will be easily readable on the film. The fine point nib is easily replaceable if it dries out.

Fine-Point Phosphorescent Marking Pen for Laboratory Autography

  • Fine Point Nib- Provides fine writing and greater information density
  • Specially Designed- Ideal glow intensity and afterglow duration helps prevent over-andunder exposure
  • No Ink Clogs- Low ink viscosity and porous nib prevents ink from clogging
Item#:
GLWR-1000
Your Price:
72.95
Each

Replacement Nibs, for

Glow Writer, 4/pk

Glow Writer is a fine-point phosphorescent ink marking pen for making experimental notations and other markings on chemiluminescent and radioisotope-labeled substrate materials (e.g., transfer membranes, dried gels, genomic and proteomic matrices, etc.) being analyzed by X-ray film autography or electronic imaging. The unique marker delivers a phosphor-containing ink through a fine point porous nib. Before exposure to X-ray film, the phosphorescent writing is excited by room light. A phosphor ink having an optimum concentration of phosphor and a relatively short afterglow halflife (approximately 2-3 minutes) has been selected so that upon exposure to X-ray film,autographic exposures of almost any duration will be easily readable on the film. The fine point nib is easily replaceable if it dries out.

Fine-Point Phosphorescent Marking Pen for Laboratory Autography

  • Fine Point Nib- Provides fine writing and greater information density
  • Specially Designed- Ideal glow intensity and afterglow duration helps prevent over-andunder exposure
  • No Ink Clogs- Low ink viscosity and porous nib prevents ink from clogging
Item#:
GNIB-1000
Your Price:
11.58
Each
  • Molded, durable construction
  • Rubber gates for easy, tapeless gel casting
  • Adjustable height combs
  • Loading guides aid in well visualization

Enduro Horizontal Gel Boxes have been designed for ease of use, safety and durability. A variety of sizes are available, from a mini gel system to a large maxi gel system that can accommodate as many as 450 samples in a single run. A wide range of accessories are available to customize your system, including multichannel compatible gel combs.

QUALITY CONSTRUCTION - The Enduro gel tanks are molded for leakproof performance and a long service life. The corrosion resistant platinum electrodes are located in cassettes to protect them during cleaning and provide for easy replacement. The lids fit on the gel boxes in only one direction. A drip ring on the lid recycles condensed buffer and prevents it from dripping directly onto the gel.

EASE OF USE - Casting gels for the Enduro system is very easy. Simply snap the rubber gates onto the ends if the tray, place the comb in position and pour the gel - no taping is necessary. Slots on the sides of the gel trays hold the combs in place. The two piece combs are designed so that the teeth can be raised or lowered to vary the depth of the well within the gel. The supplied combs are 1.5 mm thick. Combs of other thicknesses are available separately. After the gel has hardened, the combs and gates are removed and the gel and tray placed in the gel box. Loading strips may be placed on the bottom of the gel box to aid in well visualization. Gel trays are UV transparent.

DESIGNED FOR SAFETY - The power leads for the Enduro system are attached to the gel box lid. The connection with the electrodes is made only when the lid is properly in place. Removal of the lid removes power from the system. Side tabs and pressure pads make lid removal easy and prevent sloshing of buffer.

Product Specifications
Enduro 7.7
Unit Dimensions 9 x 21 x 9cm
Gel Size 7 x 7cm
Maximum Samples 32
Buffer Volume 225mL
Enduro 7.10
Unit Dimensions 9 x 21 x 9cm
Gel Size 7 x 10cm
Maximum Samples 64
Buffer Volume 225mL
Enduro 10.10
Unit Dimensions 12.5 x 22 x 9cm
Gel Size 10 x 10cm
Maximum Samples 100
Buffer Volume 300mL
Enduro 15.10
Unit Dimensions 17.5 x 26.5 x 9cm
Gel Size 15 x 10cm
Maximum Samples 140
Buffer Volume 500mL
Enduro 15.15
Unit Dimensions 17.5 x 26.5 x 9cm
Gel Size 15 x 15cm
Maximum Samples 210
Buffer Volume 500mL
Enduro 20.20
Unit Dimensions 23 x 39.5 x 9cm
Gel Size 20 x 20cm
Maximum Samples 450
Buffer Volume 1200mL
Construction Molded Acrylic
Safety Unidirectional safety lid
UV Transparency Gel tank, trays and lid
Gel Casting Rubber gates supplies
Combs Height-adjustable
Comb Thicknesses 0.75, 1, 1.5 and 2mm

Combs for E1015-10 & E1015-15

Item#:
ASPCRELCTP20
  • Molded, durable construction
  • Rubber gates for easy, tapeless gel casting
  • Adjustable height combs
  • Loading guides aid in well visualization

Enduro Horizontal Gel Boxes have been designed for ease of use, safety and durability. A variety of sizes are available, from a mini gel system to a large maxi gel system that can accommodate as many as 450 samples in a single run. A wide range of accessories are available to customize your system, including multichannel compatible gel combs.

QUALITY CONSTRUCTION - The Enduro gel tanks are molded for leakproof performance and a long service life. The corrosion resistant platinum electrodes are located in cassettes to protect them during cleaning and provide for easy replacement. The lids fit on the gel boxes in only one direction. A drip ring on the lid recycles condensed buffer and prevents it from dripping directly onto the gel.

EASE OF USE - Casting gels for the Enduro system is very easy. Simply snap the rubber gates onto the ends if the tray, place the comb in position and pour the gel - no taping is necessary. Slots on the sides of the gel trays hold the combs in place. The two piece combs are designed so that the teeth can be raised or lowered to vary the depth of the well within the gel. The supplied combs are 1.5 mm thick. Combs of other thicknesses are available separately. After the gel has hardened, the combs and gates are removed and the gel and tray placed in the gel box. Loading strips may be placed on the bottom of the gel box to aid in well visualization. Gel trays are UV transparent.

DESIGNED FOR SAFETY - The power leads for the Enduro system are attached to the gel box lid. The connection with the electrodes is made only when the lid is properly in place. Removal of the lid removes power from the system. Side tabs and pressure pads make lid removal easy and prevent sloshing of buffer.

Product Specifications
Enduro 7.7
Unit Dimensions 9 x 21 x 9cm
Gel Size 7 x 7cm
Maximum Samples 32
Buffer Volume 225mL
Enduro 7.10
Unit Dimensions 9 x 21 x 9cm
Gel Size 7 x 10cm
Maximum Samples 64
Buffer Volume 225mL
Enduro 10.10
Unit Dimensions 12.5 x 22 x 9cm
Gel Size 10 x 10cm
Maximum Samples 100
Buffer Volume 300mL
Enduro 15.10
Unit Dimensions 17.5 x 26.5 x 9cm
Gel Size 15 x 10cm
Maximum Samples 140
Buffer Volume 500mL
Enduro 15.15
Unit Dimensions 17.5 x 26.5 x 9cm
Gel Size 15 x 15cm
Maximum Samples 210
Buffer Volume 500mL
Enduro 20.20
Unit Dimensions 23 x 39.5 x 9cm
Gel Size 20 x 20cm
Maximum Samples 450
Buffer Volume 1200mL
Construction Molded Acrylic
Safety Unidirectional safety lid
UV Transparency Gel tank, trays and lid
Gel Casting Rubber gates supplies
Combs Height-adjustable
Comb Thicknesses 0.75, 1, 1.5 and 2mm

Combs for E1015-10 & E1015-15

Item#:
ASPCRELCTP19

Autoradiography Cassette Aluminum 5x7

Classic Push-Button Cassettes

  • Constructed From Lightweight Aluminum & Nylon
  • Special Curved Door Gives Exceptional Screen-Film Contact
Item#:
ASCASSETTES
IB01010
6X Loading Dye

    • Used for agarose electrophoresis of DNA, RNA or nucleic acids
    • Contains 3 tracking dyes and 15% Ficoll in a special Tris dye
      • Light blue - around 4000bp in 1% agarose
      • Indigo - around 600bp in 1% agarose
      • Magenta - around 150bp in 1% agarose
    • DNase/RNase/Protease free

IB01015
5X RNA Gel Loading Kit

  • Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
  • RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
  • DNase/RNase/Protease free

IB01190
2X Protein Loading Dye

    • Tracks the migration progression of your sample during polyacrylamide electrophoresis
    • Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
    • DNase/RNase/Protease free

IB72120
Xylene Cyanol FF

    • Used as a tracking dye at 5kb to monitor the progress of electrophoresis separation
    • CAS Number: 2650-17-1
    • DNase/RNase/Protease free

IB74040
Bromophenol Blue

  • Tracking dye in electrophoretic separations
  • Migrates around 0.5kb
  • CAS Number: 115-39-9
  • Purity: >98.0%
Item#:
ASGELLDDYES
  • Perfect box for every blot
  • Reduce antibody usage
  • Hinged Lid minimizes evaporation
  • For halves, wholes, strips, or minis

No matter what size your Western blot is, there's a box that fits it perfectly. With boxes that are correctly matched to common blot sizes, you can fit more blots on shakers/rockers while keeping reagent and antibody usage to an absolute minimum. Additionally, all corners are formed at 90° angles and the bottom surface is uniformly flat, further reducing consumption of costly reagents and antibodies (for example, popular mini-gels require only about 4mL).


At a price that makes them truly disposable, all sizes are engineered from crystal clear polystyrene. The exception is our opaque black boxes (except WBB7BK and WBB15BK), which protect light sensitive samples. All sizes are also equipped with an easy-to-open hinged lid that reduces evaporation.


Ordering Information


Item #

Description

Minimum Vol

Dimensions

WBB3

Western Blot Box, Micro-strip, Clear, 5/pk

1 mL

7.3 x 3.0 x 1.9 cm

WBB5

Western Blot Box, Mini-rectangle, Clear, 5/pk

3 mL

7.3 x 5.1 x 3.2 cm

WBB7

Western Blot Box, Medium Rectangle, Clear, 5/pk

4 mL

8.9 x 6.5 x 2.5 cm

WBB7BK

Western Blot Box, Medium Rectangle, Black, 5/pk

4 mL

8.9 x 6.5 x 2.5 cm

WBB9

Western Blot Box, Long Strip, Clear, 5/pk

2 mL

9.5 x 3.0 x 1.6 cm

WBB11

Western Blot Box, Medium Strip, Clear, 5/pk

3 mL

11.1 x 5.2 x 4.7 cm

WBB13

Western Blot Box, Large, Clear, 5/pk

6 mL

11.7 x 8.9 x 2.8 cm

WBB15

Western Blot Box, Square, Clear, 5/pk

7 mL

11.4 x 11.4 x 3.2 cm

WBB15BK

Western Blot Box, Square, Black, 5/pk

7 mL

11.4 x 11.4 x 3.2 cm

WBB17

Western Blot Box, Extra Large, Clear, 5/pk

11 mL

15.2 x 10.2 x 3.2 cm

WBB19

Western Blot Box, XXL, Clear, 5/pk

12 mL

15.2 x 10.2 x 5.1 cm

Item#:
ASBLOTBX2

Western Lightning Plus (NEL103):
·  Twice the sensitivity of standard products 

·  Direct protocol transfer 

·  ~ 1 picogram sensitivity

·  > 2 hr signal duration for re-exposing film

·  Working solution stability 8 hr at room temperature 

·  PVDF or Nitrocellulose membranes 

·  Non-toxic (no msds required)

 

Western Lightning Pro (NEL120)
·  Mid-range sensitivity, with broad dynamic range

·  Detection of less than 1 picogram amount of protein

·  Long, stable signal > 12 hr for re-exposing film 

·  Stable at room temperature >1 year 

·  Working solution stable for > 24 hr at room temperature

·  Non-toxic (no msds required) 

·  PVDF or Nitrocellulose membran
Item#:
ASWESTERNKITE
  • POWER- 150v/400ma
  • CONTROL- set voltage, amperage and time
  • FLEXIBILITY - runs 6x6cm to 12.5x12cm gels
  • THROUGHPUT - up to 112 samples at once
  • CONVENIENCE - casting set and combs included
  • WARRANTY: 1 year

Labnet's Enduro Gel XL includes all components necessary to cast and run horizontal gels: gel trays, combs, casting stand, gel tank and digital power supply. The migration tank, gel trays and multichannel combs are molded for leakproof performance and a long service life. The direct connection of the power supply into the gel box eliminates the need for power leads.

Pressing the eject button separates the gel box and power supply. Reconnecting is easy-just press the two pieces together. The power supply can be set to either constant voltage or constant current with automatic crossover between the two parameters. Parameters are shown on the large digital display that is easily visible from a distance.

There are three different gel options for the Enduro Gel XL; 12.5 x 12cm, 12.5 x 6cm and 6 x 6cm. One larger gel with multiple combs will accommodate up to 112 samples. Or run two small gels or four micro gels at the same time. Cassettes protect fragile electrodes from damage and allow for easy replacement.

A magnetic sensor allows current to flow to the electrodes only when the lid is in place. If the lid is removed while the system is operating, current is immediately shut down. In addition, the lid of the gel box is vented to dissipate heat produced during electrophoresis. To aid in visualizing the gel while it is running, there is a clear window over the gel platform.

Product Specifications 

Overall dimensions (WxLxH) 24.5 x 17.0 x 6.2 cm
Gel tank dimensions (WxLxH) 18.3 x 16.4 x 5.6 cm
Power supply dim. (WxLxH) 7.5 x 17.0 x 6.2 cm
Maximum sample capacity 112 (4-28 tooth combs)
Gel capacity Per run
Large (12.5 x 12 cm) 1 each
Small (12.5 x 6 cm) 2 each
Micro (6 x 6 cm) 4 each
Buffer capacity 300mL
Input voltage AC 100-240V, 50/60 Hz
Output voltage 10 - 150 V
Output current 10 - 400 mA
Timer up to 99 hours or continuous
Memory Last programmed parameters saved
Item#:
ASPCRELCTP21
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