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TPP Cell and Tissue Culture PlasticWare

Packed Cell Volume Counting Tubes

TPP's Packed Cell Volume (PCV) tube enables a quick and reproducible method of cell counting based on cell volume. A quick centrifuge of the cell suspension pipetted into a PCV tube reveals a packed cell pellet in the PCV capillary, which can be easily read with the aid of an Easy read ruler. This non-subjective method is superior to old-fashioned and labor intensive hemacytometer based counting.

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  • Cell suspension sample can be directly quantitated without dilution
  • Multiple samples can be assayed simultaneously
    • Load and spin as many PCV tubes as your centrifuge rotor holds
  • Quick and reproducible
    • Cells can be spun down in 1 minute at 2500xg

Ergonomic Features:

  • Reproducible measurements
    • Graduated capillary model offers immediate quantitation
    • For finer quantitation: The Easy Read Ruler is used with non-graduated PCV tubes
  • Eliminates expensive automated counters / fragile slides
    • Avoids subjective microscope counting

Quality Assurance:

  • Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
  • Individually wrapped in peel-off plastic wrapper

Protocol Example:

  • Uniformly mix cell suspension collected from TPP flask/dish/bioreactor
  • Pipet 1000µL of cell suspension into a PCV tube
  • Spin multiple samples for 1 minute at 2500xg in a standard microcentrifuge
  • Record pellet volume and compare to pre-generated standard curve of cell numbers to packed cell volume.
    • Standard curve needs to be set up only once per cell line
    • Pipet pre-determined cell suspension volumes (dilutions) into a hemacytometer
    • Calculate and record the number of live cells for each concentration
    • Pipet the identical volumes into the PCV tube, centrifuge and record the numeric in the capillary that corresponds to pellet volume
    • Plot the hemacytometer readings vs. the PCV readings for each volume
    • This Standard Curve doesn't need to be redone for the same cell line and moving forward the cell suspension harvested from TPP flasks/dishes/plates only need to be centrifuged in PCV tubes and assayed for cell pellet measurements

High density of cells

  • With a very high density of the cells, the samples must not be diluted. You might simply reduce the sample volume. Samples from the same series should be treated accordingly.
  • For bacteria measurement the parameters have to be adjusted, for example higher g-force, smaller volume, etc.
  • Micro centrifuges with swing-out rotors are preferable.

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Publication

Order# Description Quantity
TP87005

PCV Cell Counting Tubes (with capillary graduations only)

50/pk

TP87007

PCV Cell Counting Tubes (no graduations)

50/pk

TP87008

TPP Cap for PCV Tubes

50/pk

TP87010

Easy Read Ruler

1

 

TPP Cultureware from MIDSCI® offers both value and performance.

  • Flatter, more consistent growth surface for a more consistent monolayer
  • Insulated vents ensure even temperature distribution when stacked
  • Ridged gripping surface on dishes and plates facilitate handling High visibility, painted alphanumerics, graduations and writing surfaces
  • 15, 50 and 600 ml Bioreactor Tubes - a simple, disposable format for suspension of cells
  • 150 and 115 cm2 flasks offer re-closable lids
  • T-10 Flasks/Tubes - centrifuge in 50 ml rotor, and view under a microscope