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4titude plates and strip tubes

Why choose 4titude? 4titude plates and strip tubes utilize polycarbonate for plate rigidity and low-binding polypropylene for PCR tubes in their two component design.

This combination decreases thermal expansion (Figure 1) observed in 1 piece PCR products.

Thermal expansion results in volume loss due to evaporation (Figure 2), which can impact pH and the ability of the enzymes to function optimally.

4titude allows you to downscale PCR reactions, due to lower evaporation (Table 1) and save money.

Various styles of plates and strip tubes to fit your thermal cycler needs!

Try 4titude plates and strip tubes today from MIDSCI!

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  • Warp much less during PCR compared with standard plates
  • Beneficial for robotics use (more reliable with automated pipetting and stacking)
  • Less thermal expansion and shrinkage for improved sealing integrity!
  • Less sample evaporation, especially from corner wells---Saves money!
  • Beneficial for low volume PCR
  • Try our white or black wells to optimize your PCR further. Ask MIDSCI today!

Legends for Figures

Figure 1: Thermal expansion of one-piece polypropylene plates. Polypropylene plates expand by up to 2mm during thermal cycling. Seal film or caps expand at different rates, leaving an opening for evaporation.

Figure 2: Illustration of Evaporation loss. Evaporation occurs most in the outside wells, increasing variability among wells and having an impact on pH and enzyme efficiency.

Table 1: Volume loss and money saved with 4titude Framestar plates compared to 1 piece polypropylene plates. Framestar saves money every run.

Table 2: 4titude products allow reaction volumes to be reduced due to less evaporation. Cost savings were found to be $31 per 96 well plate for one customer as their Sybr Green usage was reduced by 40% with comparable quantifications among all volumes.