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Isohelix™ DNA/RNA Buccal Mouse Swabs

Item #: ASSBUCCCOLL2 
Bullseye

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Bullseye PREMIUM GC Rich Optimized DNA Polymerases and Kits

Bullseye offers a product series specifically developed for the amplification of GC-rich DNA sequences. The Bullseye HS DNA Polymerase combined with GC Buffer I and GC Buffer II promote excellent amplification results with targets of varying degrees of GC content. Hot Start DNA Polymerase is a modified form of Bullseye Taq DNA Polymerase. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 10-15 minutes heat activation step, releasing the active Hot Start DNA Polymerase into the reaction. The GC Buffers in combination with Hot Start DNA Polymerase and the heat activation step result in a high success rate in amplification of DNA targets with high GC content.

Key Features

  • Amplification of multiple DNA targets with high GC content
  • High specificity, sensitivity and product yield
  • Diminished formation of non-specific product
  • Detection of low copy number targets

Kit Components

  • HS DNA Polymerase in Storage Buffer
  • 5 U/ml Hot Start DNA Polymerase, 20 mM Tris-HCl pH 8.9, 100
  • mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20,
  • 0.5% NP40, 50% glycerol.
  • 4x GC Buffer I
  • Optimized buffer components, 6 mM MgCl
  • 4x GC Buffer II
  • Optimized buffer components, 6 mM MgCl
  • MgCl
  • 25 mM MgCl in PCR grade water

PNPCRREAG

Magnesium
Mg
Dye
No Dye
Enzymes
Premium
Pkg
Indiv
Type
Hot-Start GC Rich
High Fidelity Polymerase
Item#:
ASACCREAG4
 
High Fidelity Hot Start M
Item#:
ASACCURISHIFIDHS
 
SKU Number Volume of Tube Included Swab Diameter Qty Price Quantity Add to Cart
MS-01R 1 swab 6 mm 100
308.45
Each
MS-02R 1 swab 6 mm 250
582.42
Each
MS-03R 1 swab 6 mm 500
880.07
Each
MS-04R 1 swab 6 mm 600/cs
729.45
Each
DNA/RNA Buccal Mouse Swab
Isohelix™ DNA/RNA Buccal Mouse Swabs
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