Home  >  PCR, Protein, RNA, DNA  >  PCR  >  Reagents  >  PCR Reagents  > PR1MA™ Cod Uracil-DNA Glycosylase

PR1MA™ Cod Uracil-DNA Glycosylase

Item #: ASPR1MACODURACIL 
PR1MA Enzyme Container

PR1MA™ Cod Uracil-DNA Glycosylase

 

Is amplification carry-over contamination ruining your assay sensitivity?

 

PR1MA™ Cod Uracil-DNA Glycosylase (cUNG) is a recombinant, thermolabile enzyme that removes uracil from DNA. It is ideal for preventing carry over contamination during RNA or DNA amplification reactions that substitute dUTP for dTTP. cUNG is the only commercially available UNG that is completely and irreversibly inactivated by moderate heat treatment, unlike bacterial versions of the enzyme. Cod UNG treatment in combination with targeted pre-amplification using dUTP provides a simple and efficient solution to eliminate carry-over contamination and the generation of false positives and inaccurate quantification.

  • Optimal temperature: 37 °C
  • Heat inactivation: 55 °C for 5 minutes
  • Enables contamination control in PCR and other amplification methods
  • Does not degrade product after inactivation, enabling downstream use of the amplicon
  • Storage temperature: -20 °C

Buffer composition

  • 50% glycerol
  • 50 mM Tris-HCl
  • 50 mM KCl
  • 1 mM DTT
  • 0.1  mM EDTA
  • 0.1% Tween-20
  • pH = 7.5

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.   

 
Item#:
ASPRIMARNACNTRL
 
SKU Number Concentration Qty Price Quantity Add to Cart
PR-CODU-SM-2000 50 Unites / uL 2,000 Units
280.00
Each
PR-CODU-LG-10000 50 Unites / uL 10,000 Units
1120.00
Each
PR1MA Enzyme Container
PR1MA™ Cod Uracil-DNA Glycosylase
Special Order
Quantity: