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Bullseye Taq Plus Master Mix

While Supplies Last!
Item #: ASPCRREAG13 
RT (reverse transcriptase


This brand is being discontinued and will only be available while supplies last.


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  • Pre-optimized ready-to-use PCR reagents
  • Less pipetting to avoid contamination
  • Quick and easy to set up
  • Direct loading for electrophoresis
  • Reproducible results

Users only need to add templates and primers, and water if needed. Extra 25mM magnesium solution is also provided for additional fine adjustments. The Red Mixes contain a non-hazardous inert red dye. The purple mixes contain a non-hazardous inert purple dye. Completed PCR reactions utilizing the D124-R (red dye) or D124-P (purple dye) can be directly loaded into a well of agarose gel or other gels, for electrophoresis. No extra loading buffer is needed.

Storage: 4°C for up to one month, or -20°C for long term storage.

Magnesium Chloride: In general, 1.5mM MgCl2 is recommended; this may vary with different conditions and primer sets. Some primers/templates may require adjustments for MgCl2 concentration, which can be achieved as shown below:


Final MgCl2 concentration

Additional 25mM MgCl2 per 50µl reaction

1.5mM

0 µl

2.0 mM

1.0 µl

2.5mM

2.0 µl

Directions for use: For a 50µl reaction, use 25µl of the Taq Master Mix, add template, primers, and water to a final volume of 50µl. Cycling conditions vary for different templates and primers. To start with, try 30 cycles as follows: denature at 94°C for 30 seconds, anneal around 55°C for 30 seconds, and extend at 72°C for 1 minutes/kb. After the PCR cycles, extend at 72°C for another five minutes to complete the PCR. Then store the reaction at 4°C.

1x Composition: 10mM KCl, 20mM Tris HCl (pH9.0), 16mM (NH4)2SO4, 0.1% Triton X-100, 1.5mM MgCl2, 200mM dNTPs, 2.5units/25ul of Taq DNA polymerase, (optional: trace amount of red or purple dye) and enzyme stabilizers.

PNPCRREAG

Magnesium
Mg
Enzymes
Standard
Pkg
Mix
Type
Standard
Item#:
ASACCREAG1
 
SKU Number Vol Magnesium Dye Enzymes Pkg Type Price Quantity Add to Cart
D-124A 200 rxn Mg No Dye Standard Mix Standard
134.20
Each
D125 120 rxn Mg Red Dye Standard Mix Standard
121.11
Each
  Item is no longer available.  
RT (reverse transcriptase
Bullseye Taq Plus Master Mix
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