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Ratiometric Potassium Flux
> ION Biosciences PBFI AM
ION Biosciences PBFI AM
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ASPBFIAM
ION Biosciences PBFI AM
PBFI is a UV-excitable, green ratiometric indicator for intracellular potassium (K) measurements. Ex/Em: 340/505 nm can be used to measure K-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K-free PBFI. It is 1.5X more selective for K over Na, and works well in cytosolic conditions where [K]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
PBFI is a UV-excitable, green ratiometric indicator for intracellular potassium (K) measurements. Ex/Em: 340/505 nm can be used to measure K-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K-free PBFI. It is 1.5X more selective for K over Na, and works well in cytosolic conditions where [K]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
ION Biosciences PBFI AM
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