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Home > Liquid Handling > Pipettor Calibration > Pipettor Calibration | MIDSCI > MIDSCI Pipettor Calibration

MIDSCI Pipettor Calibration

Item #: ASMIDSCICALIBRATE

Efficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:	up to 300mg agarose gel slice up to 100µl PCR product or other enzymatic reaction
Format:	Spin columns
Operation:	Centrifuge/Vacuum Manifold
Binding Capacity:	10µg DNA
Expectant Yield:	80-90% for gel extraction 90-95% for PCR clean-up
Operational Time:	15min. for PCR clean-up/20min. for gel extraction
Applications:	PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations