Error:

Javascript is disabled in this browser. This page requires Javascript. Modify your browser's settings to allow Javascript to execute. See your browser's documentation for specific instructions.

Home > PCR, Protein, RNA, DNA > PCR > PCR Supplies > PCR Plates > Labcon PurePlus® PCR Plates

Labcon PurePlus® PCR Plates

Item #: ASLABPUREPLS

These items have been discontinued and will only be available while supplies last.
Need a great Reverse Transcriptase product at a great price? Try PR1MA!

Click here for PCR or here for qPCR to order.

Bullseye EasyScript™ Reverse Transcriptase

Application

Synthesis cDNA froma single-stranded RNA or DNA primer extension
Sequencing dsDNA
cDNA library
Template production for use in PCR
3'-end labeling of duplex DNA via end-filling reactions

EasyScript™ Reverse Transcriptase is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript™ and EasyScript™ Plus are RNase H deficient (negative). The enzyme is purified from bacteria h o s t as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components

Components	EasyScript Reverse Transcriptase
Item #	G231	G232
EasyScript RTase (200 U / uL)	5,000 U	20,000 U
5x RT buffer	150 uL	600 uL
Size	25 rxns	100 rxns

Storage Buffer

50 mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage

Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended.

1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes.
2. Prepare the following reaction mixture in a PCR tube on ice:

	Volume	Concentration (final 20 uL)
Total RNA, or poly(A)+RNA	Variable	0.5-5µg per reaction 50ng-0.5 uG per reaction
Oligo(dT) (10 uM)	1 uL	0.5 uM
or Random Primer (10 uM)	1 uL	0.5 uM
or Sequence-specific Primer	Variable	10-15 pM
dNTP (10mM)	1 uL	500 uM
5X RT Buffer	4 uL	1X
RNasin (40 U/ uL)	0.5 uL	20 U per reaction
EasyScript™ RTase (200 U/ uL)	1 uL	200 U per reaction
RNase-free H2O	Variable	-
Final volume	20 uL	-

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes.
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product.
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a randomprimer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.

PNCDNART

Pkg

Indiv

SKU Number	Size	Color	Qty	Skirt	# of Wells	Price	Quantity	Add to Cart
3961-520-000-9	0.1 ml	Natural	10 packs of 10 plates	Non-Skirted	96-Well	615.4300 615.43 Each
3962-520-000-9	0.1 ml	Natural	10 packs of 10 plates	Full Skirted	96-Well	615.4300 615.43 Each
3962-521-000-9	0.1 ml	Opaque White	10 packs of 10 plates	Full Skirted	96-Well	676.5900 676.59 Each
3963-520-000-9	0.2 mL	Opaque White	10 packs of 10 plates	Half-Skirted	96-Well	655.9100 655.91 Each
3967-520-000-9	0.2 mL	Opaque White	10 packs of 10 plates	Flat Plate	96-Well	615.4300 615.43 Each
3968-520-000-9	0.2 mL	Opaque White	10 packs of 10 plates	Skirted	96-Well	665.6300 665.63 Each
3971-520-000-9	0.1 ml	Natural	5 packs of 20 plates	Non-Skirted	96-Well	615.4300 615.43 Each
3972-520-000-9	0.2 mL	Natural	10 packs of 10 plates	Half-Skirted	96-Well	615.4300 615.43 Each
3973-520-000-9	0.2 mL	Natural	10 packs of 10 plates	Half-Skirted	96-Well	615.4300 615.43 Each
3973-529-000-9	0.2 mL	Assorted Colors	10 packs of 10 plates	Half-Skirted	96-Well	525.3400 525.34 Each
3976-520-000-9	0.1 ml	Natural	10 packs of 10 plates	Half-Skirted	96-Well	615.4300 615.43 Each
3977-520-000-9	0.2 mL	Natural	10 packs of 10 plates	Skirted	96-Well	615.4300 615.43 Each
3977-522-000-9	0.2 mL	Yellow	10 packs of 10 plates	Skirted	96-Well	526.3000 526.30 Each
3977-526-000-9	0.2 mL	Purple	10 packs of 10 plates	Skirted	96-Well	526.3000 526.30 Each
3977-527-000-9	0.2 mL	Blue	10 packs of 10 plates	Skirted	96-Well	526.3000 526.30 Each
3977-528-000-9	0.2 mL	Green	10 packs of 10 plates	Skirted	96-Well	526.3000 526.30 Each
3977-529-000-9	0.2 mL	Assorted Colors	10 packs of 10 plates	Skirted	96-Well	537.8500 537.85 Each
3978-520-000-9	0.1 ml	Natural	10 packs of 10 plates	Skirted	96-Well	615.4300 615.43 Each
3978-520-010-9	0.1 ml	Natural	10 packs of 10 plates	Skirted	96-Well	452.4700 452.47 Each
3978-522-000-9	0.1 ml	Yellow	10 packs of 10 plates	Skirted	96-Well	474.7500 474.75 Each
3978-524-000-9	0.1 ml	Red	10 packs of 10 plates	Skirted	96-Well	474.7500 474.75 Each
3978-526-000-9	0.1 ml	Purple	10 packs of 10 plates	Skirted	96-Well	474.7500 474.75 Each
3978-527-000-9	0.1 ml	Blue	10 packs of 10 plates	Skirted	96-Well	474.7500 474.75 Each
3978-528-000-9	0.1 ml	Green	10 packs of 10 plates	Skirted	96-Well	474.7500 474.75 Each
3978-529-000-9	0.1 ml	Assorted Colors	10 packs of 10 plates	Skirted	96-Well	474.7500 474.75 Each
3979-520-000-9	0.2 mL	Natural	10 packs of 10 plates	Half-Skirted	96-Well	615.4300 615.43 Each
3983-520-000-9	25 uL	Natural	10 packs of 10 plates	Skirted	384-Well	787.8000 787.80 Each
3983-520-010-9	25 uL	Natural - Barcoded	10 packs of 10 plates	Skirted	384-Well	618.4600 618.46 Each
3984-520-000-9	25 uL	Natural	10 packs of 10 plates	Skirted	384-Well	843.3000 843.30 Each