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Laser Cryo-Tag® Cryo-Babies® Sheets

Item #: ASLCRYSTS 
IBI Total DNA Extraction

Introduction
IBI Plant Isolate provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP. Phenol extraction is not required and the entire procedure can be completed within 50 minutes.

Quality Control
IBI Plant Isolate is tested on a lot-to-lot. 50 mg of fresh Arabidopsis leaves are initially ground in IBI Plant Isolate. A 15 µL aliquot of extracted genomic DNA from a µL eluate is analyzed by electrophoresis on a 1% agarose gel.

Advantages

  • High molecular weight genomic DNA extraction from a variety of plant species 
  • Sample: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
  • Scalable, simple and gentle CTAB and chloroform based DNA precipitation method
  • Cost effective

    Applications
    PCR, Real-Time PCR, Southern Blotting, Mapping and RFLP

    Caution
    IBI Plant Isolate contains irritants. During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.

    Additional Requirements
    Mortar and pestle, 1.5 mL microcentrifuge tubes or 15 mL centrifuge tubes, absolute ethanol for preparing 70% ethanol in water, chloroform, isopropanol, TE buffer or ddH2O

    Components and Storage

    Item

    Product

    Volume

    RNase A
    (50mg/mL)

    Shipping

    Storage

    IBI Plant Isolate &
    RNase A

     

    IB47610

    4 mL

    N/A

    Room Temperature

    Plant Isolate
    Dry at room temperature (15-25°C) for up to 1 year

     

    RNase A
    4°C for extended periods

     

    IB47611

    100 mL

    50 µL

     

    IB47612

    200 mL

    100 µL

PNRNADNA

Isolation type
DNA
Source Cell
Cultured Cell
SKU Number Color Qty Size Temp Usage Type Price Quantity Add to Cart
LCRY-2380 White 2380/pk 0.94 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-2380-R Red 2380/pk 0.94 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-2380-O Orange 2380/pk 0.94 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-2380-Y Yellow 2380/pk 0.94 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-2380-G Green 2380/pk 0.94 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-2380-B Blue 2380/pk 0.94 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-1400 White 1600/pk 1 x 1 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-1700 White 1700/pk 1.28 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-1700-R Red 1700/pk 1.28 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-1700-O Orange 1700/pk 1.28 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-1700-Y Yellow 1700/pk 1.28 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-1700-G Green 1700/pk 1.28 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-1700-B Blue 1700/pk 1.28 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-2500 White 2,500/pk 2.5 x 0.5 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-3020 White 240/pk 3 x 2 In. -196 - 150 C Cryo-Babies
67.00
Each
LCRY-1300 White 840/pk 1.125 x 1.75 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1200 White 1,200/pk 1.50 x 0.75 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1200-R Red 1,200/pk 1.50 x 0.75 In. -196 - 150 C Cryo-Tag
60.00
Each
LCRY-1200-O Orange 1,200/pk 1.50 x 0.75 In. -196 - 150 C Cryo-Tag
60.00
Each
LCRY-1200-Y Yellow 1,200/pk 1.50 x 0.75 In. -196 - 150 C Cryo-Tag
60.00
Each
LCRY-1200-G Green 1,200/pk 1.50 x 0.75 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1200-B Blue 1,200/pk 1.50 x 0.75 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1100 White 1,040/pk 1.69 x 0.75 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1100-R Red 1,040/pk 1.69 x 0.75 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1100-O Orange 1,040/pk 1.69 x 0.75 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1100-Y Yellow 1,040/pk 1.69 x 0.75 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1100-G Green 1,040/pk 1.69 x 0.75 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1100-B Blue 1,040/pk 1.69 x 0.75 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1258 White 600/pk 2.625 x 1 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1258-R Red 600/pk 2.625 x 1 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1258-O Orange 600/pk 2.625 x 1 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1258-Y Yellow 600/pk 2.625 x 1 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1258-G Green 600/pk 2.625 x 1 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1258-B Blue 600/pk 2.625 x 1 In. -196 - 150 C Cryo-Tag
67.00
Each
LCRY-1000 White 20/pk 8.5 x 11 In. -196 - 150 C Cryo-Tag
56.20
Each
Laser Cryo-Babies White
Laser Cryo-Tag® Cryo-Babies® Sheets
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