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Item #: ASGLASSFIBER 
IBI Total DNA Extraction

Introduction
IBI Plant Isolate provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP. Phenol extraction is not required and the entire procedure can be completed within 50 minutes.

Quality Control
IBI Plant Isolate is tested on a lot-to-lot. 50 mg of fresh Arabidopsis leaves are initially ground in IBI Plant Isolate. A 15 µL aliquot of extracted genomic DNA from a µL eluate is analyzed by electrophoresis on a 1% agarose gel.

Advantages

  • High molecular weight genomic DNA extraction from a variety of plant species 
  • Sample: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
  • Scalable, simple and gentle CTAB and chloroform based DNA precipitation method
  • Cost effective

    Applications
    PCR, Real-Time PCR, Southern Blotting, Mapping and RFLP

    Caution
    IBI Plant Isolate contains irritants. During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.

    Additional Requirements
    Mortar and pestle, 1.5 mL microcentrifuge tubes or 15 mL centrifuge tubes, absolute ethanol for preparing 70% ethanol in water, chloroform, isopropanol, TE buffer or ddH2O

    Components and Storage

    Item

    Product

    Volume

    RNase A
    (50mg/mL)

    Shipping

    Storage

    IBI Plant Isolate &
    RNase A

     

    IB47610

    4 mL

    N/A

    Room Temperature

    Plant Isolate
    Dry at room temperature (15-25°C) for up to 1 year

     

    RNase A
    4°C for extended periods

     

    IB47611

    100 mL

    50 µL

     

    IB47612

    200 mL

    100 µL

PNRNADNA

Isolation type
DNA
Source Cell
Cultured Cell
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137.22
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66559 Glass Fiber Filter 142 mm 25/pk
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101.50
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66209 A/B Glass Fiber Filter 47 mm 100/pk
138.89
Each
66213 A/C Glass Fiber Filter 25 mm 100/pk
86.21
Each
66217 A/C Glass Fiber Filter 8 x 10 in. 25/pk
155.22
Each
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80.31
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66226 A/D Glass Fiber Filter 142 mm 25/pk
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111.03
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289.23
Each
66258 TCLP Glass Fiber Filter 47 mm 50/pk
96.31
Each
66256 TCLP Glass Fiber Filter 90 mm 50/pk
117.51
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66257 TCLP Glass Fiber Filter 142 mm 50/pk
266.99
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Pall® Glass Fiber Filters
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