GelNest™ Matrix

GelNest™ Matrix is derived from mouse tumor tissue and contains extracellular matrix components such as laminin, type IV collagen, heparan sulfate proteoglycans, and more. These components support cell adhesion, differentiation, and proliferation, providing signals for these processes. Additionally, they simulate the characteristics of the basement membrane in the physiological environment, enhancing the success rate and effectiveness of cell culture.

In addition to the matrix components, GelNest™ Matrix is rich in various growth factors. These growth factors promote cell differentiation, proliferation, and migration, mimicking cellular signaling pathways and interactions in the physiological environment. GelNest™ Matrix has a wide range of applications, particularly in tissue engineering, cell culture, and research. It can be used for organoid culture, stem cell differentiation, angiogenesis, migration or invasion assays, and in vivo tumor studies.

Storage and Operation Instructions

To store GelNest™ Matrix, it is recommended to keep it in a -20°C freezer until ready for use. Prior to initial use, thaw the gel and divide it into single-use portions. Store these portions in a -80°C freezer for a maximum of 2 years. Please note that GelNest™ Matrix Gel should not be stored in a frost-free freezer.

GelNest™ Matrix is in a liquid state at 4°C and transitions into a gel state at 37°C. It starts to solidify into a gel when the temperature exceeds 10°C. For optimal performance, it is advised to pre-chill the pipette tips and conduct the procedures on ice.

GelNest™ Matrix Quality Assurance

• The protein concentration is guaranteed to be between 8 - 20 mg / mL
• The gel formation performance remains stable
• There are no LDEV (Lactate Dehydrogenase Elevated Virus), bacteria, or mycoplasma present for a high safety performance
• The endotoxin level is < 10 EU / mL
• Tests for organ-like structures and stem cell culture have been successfully performed.
• Tests for angiogenesis, tumor invasion, and tumor formation have been successfully conducted 

Specifications
 

Item	Growth Factor	Phenol Red	Recommended Applications	Volume
211212	Normal	Yes	General 2D, 3D cell culture	5 mL
211222	Normal	No	Requires colorimetric identification (such as fluorescence) or sensitivity to steroids	5 mL
211232	Low	Yes	2D, 3D cell culture with higher accuracy requirements for matrix components and requires	5 mL
211242	Low	No	Colorimetric identification or sensitivity to steroids	5 mL
211252	Normal	Yes	In vivo tumor formation, thrombosis test, angiogenesis experiment, general cell culture, etc.	5 mL
211262	Normal	No	2D, 3D cell culture with higher accuracy requirements for matrix components	5 mL
211272	Low	Yes	hESC stem cell culture	5 mL
211282	Low	No	Organoid culture and differentiation	5 mL
211492	Normal	Yes	Angiogenesis Experiment	5 mL