So-Low Upright Freezers

• Sample: Variety of yeast and other fungus species
• Yield: Up to 30 µg of RNA (5 x 107 Saccharomyces cerevisiae: 20 µg)
• Convenient: Includes Sorbitol Buffer to reduce sample preparation time
• Format: RNA spin columns
• Operation Time: Within 70 minutes
• Elution Volume: 50-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 9 months

The rYeast Total RNA Mini Kit was designed for total RNA purification from yeast and a wide variety of other fungus species. Sorbitol Buffer is included with the kit to reduce sample preparation time and minimize hands on time. Detergents and chaotropic salt are used to lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. The entire procedure can be completed within 70 minutes and the purified RNA is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis.

Quality Control
The quality of the rYeast Total RNA Mini Kit is tested on a lot-to-lot basis by isolating RNA from Saccharomyces cerevisiae (5 x 10  ) harvested by centrifugation at 5,000 x g for 10 minutes. A 5 µL aliquot of purified RNA from a 50 µL eluate is analyzed by electrophoresis on a 0.8% agarose gel.

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.