Soil DNA Extraction Kit

Item #: ASDNARNAKIT26 
  • Sample Size: 250-500 mg of soil
  • Expectant Yield: up to 5 µg of genomic DNA
  • Format: beadbeating tubes, PCR inhibitor removal columns and genomic DNA spin columns
  • Operation Time: within 40 minutes
  • Elution Volume: 30-100 µl
  • Kit Storage: dry at room temperature (15-25°C) for up to 18 months without showing any reduction in performance

The Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized and disrupted using SL1 lysis Buffer combined with ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a unique inhibitor removal Buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.


Component

IB47800

IB47801

IB47802

SL1 Buffer1

4 ml

50 ml

85 ml

SL2 Buffer

1 ml

15 ml

30 ml

SL3 Buffer

10 ml

45 ml x 2

160 ml

Wash Buffer2
(Add Ethanol)

1 ml
(4 ml)

25 ml
(100 ml)

25 ml
(100 ml)

Elution Buffer

1 ml

6 ml

30 ml

Inhibitor Removal Columns

4 pcs

50 pcs

100 pcs

GD Columns

4 pcs

50 pcs

100 pcs

Beadbeating Tubes

4 pcs

50 pcs

100 pcs

2 ml Centrifuge Tubes

4 pcs

50 pcs

100 pcs

2 ml Collection Tubes

4 pcs

50 pcs

100 pcs

PNRNADNA

Source Cell
Soil
SKU Number # of Samples Isolation type Source Cell Price Quantity Add to Cart
IB47800 4 All Soil
30.11
Each
IB47801 50 DNA Soil
388.91
Each
IB47802 100 DNA Soil
701.31
Each
Soil DNA Extraction Sampl
Soil DNA Extraction Kit
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