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Home > PCR, Protein, RNA, DNA > PCR > Reagents > PCR Reagents > PREMIUM Bullseye Taq DNA Polymerase Master Mix

PREMIUM Bullseye Taq DNA Polymerase Master Mix

While Supplies Last!

Item #: ASPCRREAG3

Suggested Protocol using Taq 2x Mix

This protocol serves as a guideline for primer extensions. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.

Notes:

Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis. Work on ice at all times.

The final MgCl2 concentration of this 2x Master Mix is 1.5 mM. If more than 1.5 mM MgCl2 is required, use 25 mM MgCl2 (may be purchased separately) to adjust the Mg2+

Additional volume (µl) of MgCl2 per 50 µl reaction

Final MgCl2 conc. in reaction (mM)	1.5	2	2.5	3	3.5	4	4.5
Additional volume of 25 mM MgCl2 per reaction (µL):	0	1	2	3	4	5	6

Thaw Taq 2 Master Mix and primers. It is important to thaw the solutions completely and mix thoroughly before use to avoid localized concentrations of salt. Keep all components on ice.

The table below shows the reaction set up for a final volume of 50 m If desired, the reaction size may be scaled down. Use 10 µl of the Taq 2x Master Mix in a final volume of 20 µl.

Important: Spin Taq Master Mix vials briefly before use.

Set up each reaction as follows:

Component	Vol./reaction	Final Conc.
Taq 2x Master Mix	25 µL	1X
25 mM MgCl2	0 µl (0-7 µl)	1.5 mM (1.5-5 mM)
Primer A	1 µL (0.5-5 µl)	0.2 µM (0.1–1.0 µM)
Primer B	1 µL (0.5-5 µl)	0.2 µM (0.1–1.0 µM)
Distilled Water	Variable	– – – –
Template DNA	Variable	Genomic DNA 50 ng (10-500 ng) Plasmid DNA 0.5 ng (0.1-1 ng) Bacterial DNA 5 ng (1-10 ng)
TOTAL volume	50 µL	– – – –

Mix gently by pipetting the solution up and down a few times.

Program the thermal cycler according to the manufacturer´s instructions. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

Place the tubes in the thermal cycler and start the reaction.

Three- step PCR Program:

Cycles	Duration of Cycles	Temperature
1	2-5 minutes^a	95°C
25-35	20-30 seconds^b 20-40 seconds^c 30 seconds^d	95°C 50-65°C 72°C
1	5 minutes^e	72°C

^a Initial denaturation step. Optional, but recommended for genomic DNA

^b Denaturation step: Heating the DNA template disrupts the hydrogen bonds yielding ssDNA.

^c Annealing step: Primers anneal to ssDNA template. Typically annealing temperature is 3-5°C below the Tm of the primers.

^d Extension/elongation step: Taq DNA polymerase synthesizes a new DNA strand complementary to the DNA template. Extension time depends on length of DNA fragment to be amplified. At optimum temperature the DNA polymerase will polymerize 1000 bases per minute.

^e Final elongation step: After the last PCR cycle to ensure that any remaining ssDNA is fully extended.

TPP Tissue Culture Dishes

Key Features:

Vents provide airflow between stacked dishes for consistent growth conditions and prevention of Condensation
Surface treatment of the growth area developed by TPP optimally enhances the proliferation of the cells
Serrated grip ring on lid prevents accidental drops or lid lift offs in unsterile conditions.
Double marking areas - yellow and frosted marking spots for easy labeling.
Stacking ring on the lid and corresponding ridge on the base provide an extremely secure stacking feature
Numeric "clock" markings (12, 3, 6, 9) for quick orientation during microscopy.
Crystal clear clarity for great microscopy images.

Consistent Growth Conditions

Air vents in the dish base allow airflow between stacked dishes for consistent air flow, temperature distribution, and prevention of condensation.
Both features provide consistent growth conditions even when dishes are stacked.
Outstanding flatness of surface promotes uniform cell growth without clumping.

Notable extra features

Tissue culture dishes are designed for manual handling.
The yellow inscription field on the lid and the frosted inscription field on the base enable a defined positioning of the lid.
The side walls of the dish are not treated for tissue growth

Quality Assurance:

Made from optically clear polystyrene for excellent viewing
Sterile, packaged peel-off sleeves
Certified DNAse/RNAse, nucleic acid, and pyrogen-free

Please call us for sample!

SKU Number	Vol	Magnesium	Dye	Enzymes	Pkg	Type	Price	Quantity	Add to Cart
BE140303	500 runs	Mg	No Dye	Premium	Mix	Standard	350.4900 350.49 Each
BE180303	500 runs	Mg	Red Dye	Premium	Mix	Standard	297.8800 297.88 Each		Item is no longer available.