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Azure Biosystems Cielo qPCR System

Limited Time Promo!
Item #: ASCIELO 

1 year standard warranty reflects the robustness and reliability of the system, giving you confidence in your results.

Azure Biosystems Cielo qPCR System
     
Dyes/Probes
Cielo 3
Cielo 6
SYBR Green, EvaGreen, FAM
X
X
VIC®, HEX, JOE, CAL Fluor® 540, CAL Fluor® Orange 560
X
X
TAMRA
 
X
ROX, TEX®615, CAL Fluor® Red 610
 
X
Cy®5, Quasar®670, Liz®, Mustang Purple®
X
X
C®y5.5
 
X
 
 
 
 
Cielo 3
Cielo 6
Part #
AIQ030
AIQ060
Product description
96-well Real-Time PCR instrument with 10.2” touchscreen interface,
3 dye channel filters
96-well Real-Time PCR instrument with 10.2” touchscreen interface,
6 dye channel filters
Sample capacity (wells)
96
96
Reaction volume
10–150µL
10–150µL
Excitation source
LED
LED
Detection channels
3
6
Multiplexing
Up to 3 targets
Up to 6 targets
Thermal element
Peltier
Peltier
Max. block ramp rate
6°C/sec
6°C/sec
Avg. sample ramp rate
4°C/sec
4°C/sec
Temperature uniformity
±0.2°C
±0.2°C
Temperature accuracy
±0.1°C
±0.1°C
Custom dye/chemistry
x
x
Chemistry capability
Fast/Standard
Fast/Standard
Detection sensitivity
1 Copy
1 Copy
Sensitivity
Detect differences as small as 2-fold in target quantities
in singleplex reactions
Detect differences as small as 2-fold in target quantities in singleplex reactions
Connectivity
USB, Wi-Fi, Ethernet
USB, Wi-Fi, Ethernet

 


Azure Cielo 3 AIQ030

Azure Cielo 6 AIQ060

Dyes/Probes

SYBR™ Green, EvaGreen™, FAM™

x

x

VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor® Orange 560

x

x

TAMRA™

 

x

ROX™, TEX®615, CAL Fluor® Red 610

 

x

Cy®5, Quasar®670, Liz®, Mustang Purple®

x

x

Cy®5.5

 

x

Other dyes/probes are possible depending on the system configuration.

 


TAKE ADVANTAGE OF THIS SPECIAL PRICING! 

Azure Cielo 6 qPCR (AIQ060) PROMOTIONAL PRICE: $16,895
 

*Includes: Azure Cielo 6, analysis software, and 18 months of warranty.

 

Promo is valid 01/01/24 - 03/31/24. Not to be combined with any offers or discounts. No institutional pricing. All terms apply.


Azure Cielo 3 & 6

The Azure Cielo Real-Time PCR system provide accuracy and sensitivity needed for research, with intuitive touch screen software.

Product Features

  • Flexibility:  Engineered for a wide variety of qPCR applications, with high sensitivity and wide dynamic range.
  • Intelligent workflow: The user interface is a simple touch screen assay setup.  Protocols can be saved to make assay set up easy and reproducible.
  • Reliability: Cielo's optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1,000 qPCR experiments, giving you confidence in the reliability of the instrument.
  • Connectivity: An external PC is not required to run the instrument.
  • Data Transfer: This can be performed via Ethernet, Wi-Fi or USB.


Operation - A standalone qPCR system with 10.3” touchscreen interface
Optics - 3 or 6 dye channel filters with LED based excitation for multiplexing
Speed - Fiber optic detection system scans 16 wells simultaneously
Durability - Tested reliability for over 1000 continuous runs
Connectivity - Easily export data via Wi-Fi, ethernet, USB or LAN
Capacity - 96-well format for semi-skirted or un-skirted plates and tubes
Thermal Performance - Marlow Peltier based block with 12 temperature gradient zones, ramp rates up to 6.0°C/sec
Chemistry - Supports reaction volumes between 10 µl–150 µl, open source chemistry, including FAST ~20 minute run-times
Sensitivity - Detect 2-fold differences in target quantities in singleplex reactions and up to 1 copy of amplified DNA, 10 logs of dynamic range


Competitor Q uses a single light source to excite all samples.

Differences in light path length lead to differences in excitation, which requires normalization. Though Competitor Q has said that they do not require normalization dyes, this is not reflected in the data as the data CV values for replicates is significantly higher.

 

Why doesn’t the Cielo require ROX dye?

 The Cielo does not require ROX dye because it uses in-well detection technology. The light path for excitation and reading emission are collimated directly into the well. As such, the light path length is always the same for each well, so there is no need to normalize for varying light path length. The data from the Cielo is consistently uniform and reproducible.

 

Dyes/ProbesAzure Cielo 3 AIQ030Azure Cielo 6 AIQ060
SYBR™ Green, EvaGreen™, FAM™xx
VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor® Orange 560xx
TAMRA™ x
ROX™, TEX®615, CAL Fluor® Red 610 x
Cy®5, Quasar®670, Liz®, Mustang Purple®xx
Cy®5.5 x
Other dyes/probes are possible depending on the system configuration.
SKU Number Type # of Samples Price Quantity Add to Cart
AIQ030 Azure Cielo 3 qPCR 96
27850.00
Each
AIQ060 Azure Cielo 6 qPCR 96
34850.0000
16895.00
Each
AIQ1000 Azure Cielo 3 Software Plate Setup PCR compatible
1600.00
Each
ASCIELO
Azure Biosystems Cielo qPCR System
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