Autoclave Tape

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General Description
Bullseye GC HS 2x Master Mix II is an all-in-one 2x master mix containing HS DNA polymerase, GC Buffer II, enhancer, dNTPs and MgCl. Simply mix GC HS 2x Master Mix II with primers,template and water and you are ready to carry out successful primer extensions. HS DNA Polymerase is a modified form of Bullseye Taq DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity, increased sensitivity and greater yields when compared to standard DNA polymerases.

Key Features

• For amplification of DNA targets with high GC content
  
• Convenient reaction set-up at room temperature
  
• High specificity, sensitivity and product yield
  
• Detection of low abundance targets
  
• Diminished formation of non-specific product

  
  Composition of GC HS 2x Master Mix I
  

• HS DNA Polymerase
  
• Optimized buffer components, 3.0 mM MgCl
  
• dNTPs
  
• Enhancer

  
  Storage and Stability
  The unopened kit is stable at -20°C for 1 year