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Cryogenic Vials

Item #: ASCHROMCYRO3 

Efficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction
Format:
Spin columns
Operation:
Centrifuge/Vacuum Manifold
Binding Capacity:
10µg DNA
Expectant Yield:
80-90% for gel extraction
90-95% for PCR clean-up
Operational Time: 15min. for PCR clean-up/20min. for gel extraction
Applications:
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations

PNRNADNA

Isolation type
DNA
Source Cell
Gel
SKU Number Vol of Tube Color Thread and Bottom Qty Price Quantity Add to Cart
EX100S 10mL Clear External Thread-Skirted 500
450.83
Each
EX12S 1.2mL Clear External Thread-Skirted 500
363.44
Each
EX20R 2mL Clear External Thread-Round 500
248.92
Each
EX20S 2mL Clear External Thread-Skirted 500
231.36
Each
EX30S 3mL Clear External Thread-Skirted 500
289.59
Each
EX40S 4mL Clear External Thread-Skirted 500
259.43
Each
EX50S 5mL Clear External Thread-Skirted 500
285.55
Each
IN12S 1.2mL Clear Internal Thread-Skirted 500
343.38
Each
IN20R 2mL Clear Internal Thread-Round 500
299.51
Each
IN20S 2mL Clear Internal Thread-Skirted 500
299.51
Each
IN40R 4mL Clear Internal Thread-Round 500
351.23
Each
IN40S 4mL Clear Internal Thread-Skirted 500
348.60
Each
IN50R 5mL Clear Internal Thread-Round 500
503.89
Each
IP12S 1.2mL Clear External Thread-Skirted 500
331.28
Each
IP20R 2mL Clear External Thread-Round 500
226.90
Each
IP20S 2mL Clear External Thread-Skirted 500
205.00
Each
IP30S 3mL Clear External Thread-Skirted 1,000/cs
472.77
Each
IP40S 4mL Clear External Thread-Skirted 500
253.25
Each
IP50S 5mL Clear External Thread-Skirted 500
284.93
Each
YOINA N/A Assort Caps 500
37.64
Each
YOIN1 N/A White Caps 500
37.64
Each
YOIN2 N/A Blue Caps 500
37.64
Each
YOIN3 N/A Red Caps 500
37.64
Each
YOIN4 N/A Green Caps 500
28.22
Each
YOIN5 N/A Yellow Caps 500
37.64
Each
Cryo Series
Cryogenic Vials
Special Order
Quantity: