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Microplate Readers
Microplate readers have become a fundamental piece of equipment for scientists analyzing and processing biological and chemical data. Investing in the right plate reader can help reduce costs in the long run, as well as save time and improve processes — giving you the ability to spend more time on the data.
Whether you are looking for a single-mode or a multimode plate reader, MIDSCI can help. We carry low-cost readers that don’t sacrifice quality, performance, or reliability. We also sell the top-rated PerkinElmer microplate readers: Victor® Nivo™, EnSight, and EnVision.
Let our scientific equipment experts at MIDSCI help you select which model fits your current and anticipated needs. Simply fill out our contact form or email us at tech@midsci.com
About Microplate Readers
A microplate reader detects light signals of samples in microplates or well plates. Depending on the intended use of the plate reader, there are multiple features and functionalities to select from at varying price points. From detection modes to plate layouts, determining the right reader for your lab is critical.
If you are looking for a low-cost microplate reader for absorbance range from -0.2 to 4.000 ABS, a wide wavelength range from 340 to 750 nm, or a Luminator, we can help. These units are ideal for performing ELISAs, tube based immune assays, drug assays, and so much more.
At MIDSCI, we carry all the latest detection technologies from MIDSCI and Perkin Elmer, including:
The Victor® Nivo™ has the smallest and most affordable multimode plate reader in the industry. It is certified for use with HTRF© technology and is best for research labs with multiple users and low-throughput assays.
The EnSight is an all-in-one benchtop system for fast well imaging, Alpha and conventional detection technologies. Ideal for research and assay development labs performing cell-based assay at medium-throughput.
The EnVision is the fastest benchtop reader, certified for with HTRF© technology, and for screening or assay development labs needing high-throughput capability.
If you need a compact and economical microplate reader that works with your computer without a hitch, then the Chromate microplate reader is a good choice.
Yet if you want some convenient artificial intelligence on your side, then take a look at the Accuris microplate reader instead. It has an intuitive screen and provides measurement data, interpretations, and calculated analyses on the screen after a reading.
If you need anything from absorbance to fluorescence, email us at tech@midsci.com. Our qualified sales team will answer your questions and help you get what your need.
Search our website or contact us for more information about multi well plates, microplate washers, of anything else you might be looking for.
T4 DNA Ligase
Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. This enzyme joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Intact Genomics T4 DNA Ligase displays up to 3-5X higher ligation efficiency than the nearest competitor.
Product Source
E. coli strain expressing a recombinant clone
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Contents & Storage
- T4 DNA Ligase
- 10x T4 DNA Ligase Reaction Buffer (w/o ATP)
- 10 mM ATP
Store all contents at -20 °C.
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C
10x T4 DNA Ligase Reaction Buffer (w/o ATP)
500 mM Tris-HCl, 100 mM MgCl, 100 mM DTT, pH 7.5 @ 25 °C
Note
10x T4 DNA ligase buffer does not contain ATP. You need to add ATP separately.
Unit Definition
One Weiss unit is defined as the amount of enzyme required to convert 1 nmol of 32P from pyrophosphate into Norit-absorbance material in 20 minutes under standard assay conditions.
Protocol
- Set up reaction buffer in a microcentrifuge tube on ice. Use a molar ratio of 1:3 vector to insert DNA.
Component |
10 µl Reaction |
Vector DNA |
x µl |
Insert DNA |
x µl |
10 mM ATP |
1.0µl |
10x T4 Ligase Buffer |
1.0µl |
T4 DNA Ligase |
1.0µl |
Add H2O up to |
10.0µl |
- Gently mix the reaction and centrifuge briefly.
- For cohesive ends, incubate 16 °C for overnight or at room temperature for 30 min.
- For blunt ends, incubate 16 °C for overnight or at room temperature for 2 hrs.
- Heat inactivate at 70 °C for 15 min.
- Cool on ice and transform 2 µl of the reaction into 50 µl competent cells.
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