PURIST Ultrapure Water System

REPLACEMENT PD COLUMNS AND COLLECTION TUBES -100 PACK

• For use with leftover reagents from IBI Plasmid Kits or competitive products
• Sample Size: 1 -4 mL bacterial culture
• Elution Volume: 50 to 100 µL
• Binding Capacity: Up to 30 µg

The Replacement Mini Plasmid Columns and Collection Tubes are for use with leftover reagents from IBI Mini Plasmid Kits including the following item numbers: IB47101, IB47102, IB47170, IB47171, and IB47172.

The columns and collection tubes can also be used with comparable, competitive Mini Plasmid Kit reagents that utilize a bind, wash, and elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.

SPECIFICATIONS

Sample Size: 1 - 4 mL bacterial culture
Format: Spin column or vacuum manifold
Expected yield: 20 -30 µg for high copy and 3 -10 µg for low copy.
Elution volume: 50 µL to 100 µL
Operation time: 15 minutes
Purified plasmid DNA is ready for use in ligation, PCR, restriction enzyme digestion, and sequencing reactions.