Accuris SmartBlue™ Transilluminators

Accuris SmartBlue™ Transilluminators have been developed for viewing DNA gels stained with new, safe fluorescent dyes.  These new dyes, such as SmartGlow™, SYBR Green, Gel Green, have an excitation wavelength of 465-470 nm (visible blue light), and are safer to use than Ethidium Bromide.

• Blue light, safe for nucleic acids
• Uniform illumination (UI Technology)
• Optimal wavelength (465nm) for common safe stains
• Adjustable position, amber filter covers
• Optional amber viewing glasses: E4000-VG1

Safer for samples, safer for you: Accuris SmartBlue™ Transilluminators are the perfect partner for the most common safe DNA stains. These instruments emit blue light at a wavelength of 465 nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few.  SmartBlue™ transilluminators can also work for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images.   Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures.  In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.*

Adjustable filter cover: Both the E4101 and SB4500 transilluminators come with emission filter covers. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescing DNA bands to be seen clearly.  The covers can be set to a flat position for general gel viewing or can be set at an angle to provide easy access to the gel for band excision.  The SB4500 has a two-position filter cover, and the E4101 features a hinged filter cover.

Optional amber viewing glasses: Our E4000-VG1 SmartBlue™ Plus Viewing Glasses offer efficient blocking of the blue light wavelength from the transilluminators, allowing clear visualization of the fluorescing DNA bands without the filter cover in place.  These convenient glasses are recommended for users that are frequently excising DNA bands from gels.  SmartBlue™ Plus glasses also block 100% of UV wavelength so they can also be used for viewing gels on a UV transilluminator.

UI technology, bright and clear images: The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue™ Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.

Reliable, durable, and long-lasting: An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue™ Transilluminators. Unlike those in a UV transilluminator, the filters in the SmartBlue™ systems will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue™ Transilluminators are covered by a 2 year warranty.

* SmartBlue™ products use high intensity LED lights.  Although the visible blue wavelength is significantly safer than UV light, it is recommended to not stare directly at the viewing surface without the amber cover in place. 

Specifications 

Light Source	High Intensity Blue LEDs
Emission Filter Cover	Amber
Wavelength	Peak at 465 nm
E4000 Viewing Surface	17 x 12 cm
E4000 Ext. Dimensions	30.5 x 21.5 x 5 cm
E4101 Viewing Surface	10.5 x 10.5 cm
E4101 Ext. Dimensions	17 x 12 x 5 cm
Electrical	100 to  240V, 50-60Hz
E4000 Weight	1.2kg / 2.6lbs
E4101 Weight	0.34kg / 0.75lbs
Warranty	2 years

 
 Item # SB4500 is proudly made in the USA! Click here for more made in America products!
 

Accuris Fast Extraction PCR Kit

The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

• Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
• Compatible with animal tissue, plant, saliva, & bacterial samples
• Extraction & Amplification in less than 60 minutes
• Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
• Process extracted sample via PCR with included mastermix
• Alternatively, amplify via qPCR (qPCR mastermix not included) 

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria
 

Proudly made in the USA! Click here for more made in America products!
 

• 302 nm midrange UV wavelength (optimal for EtBr excitation)
• Dual intensity settings (50% or 100%)
• Hinged UV Safety cover
• Use with SmartDocTM enclosure (E5000-SD) for gel imaging with a smart phone
• Compatible DNA stains: EtBr, SmartGlow™ LD and PS stain, Gel Red, Gel Green, SYBR® Green, SYBR® safe, and Diamond™ Nucleic acid Dye
• 2-yr warranty

This new UV transilluminator features a space-saving design while having 16 x 19 cm UV viewing surface to accommodate large gels or multiple smaller gels. The illuminated surface is comprised of a special black glass which is easy to clean, durable and scratch resistant.

Furthermore, an array of high intensity UV bulbs coupled with an optimized filter provides even illumination across the viewing surface and allows detection of small sample quantities. The dual intensity switch can be used to lower the UV intensity to 50% to reduce DNA damage during long exposure times, or switch to high intensity (100%) for viewing weak fluorescent signals or for photo documentation.

The acrylic UV blocking cover allows safe viewing of samples and has two friction hinges on the front edge to allow angling of the cover for gel access. The cover can be positioned to 90° (straight up) to fit a gel imaging hood, such as the SmartDoc™ imaging enclosure (E5000-SD).

Specifications
 

Light Source	UV
Wavelength	302 nm
Viewing Surface	16 x 19 cm
Ext. Dimensions	10.5 x 13 x 5.5 in | 26.5 x 32.5 x 14 cm
Electrical	120 or 230V, 50-60Hz
Warranty	2 years

 

Accuris SmartReader™ UV-Vis Microplate Reader
 
The Accuris SmartReader™ UV-Vis Microplate Reader (model MR9610) has been designed with ease-of-use and fast results in mind. This instrument brings extensive capabilities to the lab bench and is designed for the busy lab with multiple users. The large touch screen command center is extremely user-friendly and allows for fast set up of new protocols, as well as modifying and running existing protocols.

• Intuitive, 10-inch color touch screen control panel
• Accepts both 96 & 384-well plates
• Monochromator-based system
  • No filters required
  • Wavelength range from 200 to 1000 nm
• Stand-alone design
  • No PC or special software required
  • USB port for flash drive data transfer

• Inert yellow dye helps reduce pipetting errors
• Room temperature stable for up to 30 days
• Compatible with fast cycling protocols
• Highly sensitive for low copy number templates
• Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.
 
 

Smart-Q Fluorometers

Quantification of DNA, RNA and protein samples

• Sensitive quantitation down to 0.1 pg/ul ds DNA
• Large color touch screen for programming and operation
• Two models for single or 8-sample processing
• Built-in reagent calculators for sample preparation
• Compact and portable (11 x 6-inch footprint)

The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving:

• Next Generation Sequencing
• PCR
• Transfection
• Western blotting
• Immunoassays

The Smart-Q Fluorometers are engineered to achieve optimal performance with Accuris’ Fluorescent DNA Quantification Kits and assay tubes; however, they also maintain compatibility with a range of kits offered by various other suppliers.

Intuitive Usability

Each instrument is equipped with a 7-inch color touchscreen, featuring an intuitive, icon-driven user interface. Preprogrammed protocols allow for quick & effortless assay set-up. Results are quickly calculated and displayed in less than 6 seconds and saved to the instrument’s internal memory. All data can also be exported easily to a PC using a USB flash drive.

Accurate Quantification

The Smart-Q Fluorometers, along with their accompanying assay kits, are engineered for the precise measurement of fluorescent dye signals that are bound to targeted biological molecules. These specially formulated dyes exhibit selective affinity towards DNA, RNA, or proteins, and generate fluorescent signals exclusively upon binding to their respective targets. Both Smart-Q models include Blue and Red channels that allow for various fluorescent dyes to be used in nucleic acid and protein measurements.

The Smart-Q Fluorometers incorporate advanced curve-fitting algorithms to establish calibration curves from standard samples of known concentrations.

The concentration of an unknown sample, whether it be DNA, RNA, or protein, is determined by correlating its Relative Fluorescence Units (RFUs) with those of the standards employed during calibration. The measurement sensitivity for each type of assay is distinct and defined by its detection limits.
 

Aspire Aspirator

• Compact Design
• Built-in Vacuum Pump
• Adjustable Vacuum Pressure
• Vacuum Gauge
• Safe and Precise Aspiration

The Accuris ASPIRE Laboratory Aspirator incorporates a quiet, maintenance-free pump inside a sturdy, yet compact base. The vacuum is fully adjustable, with a pressure gauge that displays the vacuum level, allowing full control of aspirating speed. The ASPIRE is an ideal choice for a wide range of laboratory aspirating applications, including removal of media from tissue culture vessels or plates, and supernatants. An ergonomic, hand held aspirating device is included, and holds standard 200 to 300ul disposable pipette tips.

Comes complete with:

• Base with internal vacuum pump
• 2 Liter, Polycarbonate Collection Bottle (autoclavable and break resistant)
• Antibacterial / Hydrophobic Filter
• Silicone Tubing
• Handheld Vacuum Controller
• 2 Pipette cone adapters (for 200 ul pipette tips and 40mm stainless steel)

The collection bottle incorporates a reliable Overflow Protection Valve that closes off the tube to the vacuum in the event of a full bottle. In addition, the replaceable in-line filter protects the vacuum pump from contaminants and any moisture that comes through the bottle. Graduations on the collection bottle indicate the amount of fluid collected and when it's ready to be emptied. The Handheld Vacuum Pipette has an ergonomic design that's comfortable in the hand during prolonged use.  Pressing the button applies the vacuum. The pipette accepts Pasteur pipettes, serological pipettes, and also comes with 2 accessory pipette cones. One cone is designed to accommodate 200/300ul pipette tips, the other has a thin, stainless steel capillary for fine control aspirating. 
 

Specifications