Plant genomic RNA isolation for:

The HighPrep™ Plant RNA Plus Kit is specially designed for purifying RNA from a wide range of plant and fungi species. The kit uses the special lysis condition with HighPrep™ magnetic particles technology to isolate the high-quality RNA. Our kit's unique binding conditions ensure that only RNA will bind to the magnetic particles while most of the contaminating cellular proteinaceous components are removed. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, Southern blotting, SNP analysis and NGS etc. The HighPrep™ Plant RNA Plus Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.

The HighPrep™ Total RNA Plus kit is a paramagnetic bead-based system that allows for a fast and efficient purification of high-quality total RNA from tissues and cultured cells suitable for numerous high-performance RNA downstream applications such as RT-PCR, microarrays, cDNA synthesis. As a paramagnetic bead-based system, the HighPrep™ Total RNA kit can be used in a high-throughput format, hence adaptable to numerous commercially available workstations.

The HighPrep™ DTR Clean-up System is a high performance paramagnetic bead-based reagent for BigDye™ terminator removal from Sanger sequencing reactions for reliable and long readings of DNA sequences. The HighPrep™ DTR Clean-up System consists of a selective binding of DNA to the paramagnetic beads, followed with washing off nucleotides, primers, and non-targeted amplicons, and finally elution of pure DNA. HighPrep™ DTR Clean-up System is an excellent choice for both manual and fully automated purification of sequencing reaction products. The protocol can be adapted to common liquid handling workstation.

The MagCTL Collection Tube is the first Guanidine Free, FDA 510(K) approved and CE/IVDR certified sample collection device that can stabilize DNA & RNA at ambient temperatures. The tube contains a proprietary molecular transport medium which lyses and inactivates collected samples. Direct lysis of the sample effectively stabilizes the nucleic acids (DNA & RNA) making them available for capture without the need of an additional lysis step. Furthermore, direct pathogen lysis eliminates the need for sample processing in a containment making it easier for laboratory staff to handle samples.

The long term ambient stability of DNA & RNA offered by the MagCTL Collection Tubes makes them suitable for clinical settings since it eliminates the need for freezer storage space. Samples can be stored at ambient temperature on the bench top for up to 8 Days for RNA and 45 days for DNA.

Safety - The MagCTL Collection Tube consists of 1.2 mL of the MagBio CTL™ Medium contained in a sterile 5 mL Cryotube. The proprietary medium is GUANIDINE FREE making it safe for use in clinical settings that require cleaning with bleach on a regular basis. In light of the widespread use of guanidine based collection devices due to the COVID-19 Pandemic, the FDA published this article warning sample processing facilities to avoid using guanidine based collection devices.

Stability - The MagCTL Collection Tube validation study showed that RNA is stabilized for up to 8 days at room temperature. However, further research studies on this product showed stability of both RNA and DNA. Stability of RNA was 14 days and DNA stabilization was 45 days at room temperature. This allows for samples to be collected and transported without the need for cold storage at any point.

Direct Lysis – The direct lysis feature is the most unique feature of the MagCTL Collection Tube. Collected samples are lysed upon collection and the nucleic acids are released making them available for capture. This allows end users to skip the lysis step in extraction workflows. The released DNA / RNA can be captured using the MAG-S2 collection beads manufactured by MagBio. The direct lysis also adds to safety because the virus is inactivated and during sample processing there is no need for the use of a containment.

Multi Sample Versatility – The MagCTL Collection Tube is a versatile device that can be used to collect and analyze Viral, Bacterial OR Fungal samples.

FDA & CE/IVDR Clearance – The MagCTL Collection Tube is the first non-hazardous, FDA 510(K) approved sample collection device that offers direct lysis and can stabilize nucleic acids at ambient temperatures. This makes the tube a suitable candidate for clinical laboratories performing pathogen detection or sequencing. In addition to FDA Clearance, the MagCTL Collection Tube is also CE/IVDR Certified.

Downstream Benefits – The MagCTL Collection Tube allows end users to significantly cut down time needed for downstream processes. Samples collected in the MagXtract Collection Tube skip the lysis process and are directly captured in magnetic beads (MAG-S2) followed by washes and elution in nuclease free water. The extraction efficiency of MagCTL Collection Tube is comparable to that of Thermofisher MVP II Kit where lysis buffer is used. This proves that the MagCTL Tubes can be critical in reducing both time & cost associated with nucleic acid extraction.

 

The HighPrep™ Viral RNA Kit is designed for rapid and reliable isolation of viral nucleic acids from serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.