Green fluorescent, calcium (Ca²+) indicator, membrane impermeable.

 

Fluo-4 is the most popular green fluorescent, calcium (Ca²+) indicator. Proven and trusted for decades to measure intracellular Ca²+ flux in high throughput screening and fluorescence microscopy applications.

Fluo-4 K+ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Green fluorescent, calcium (Ca²+) indicator, membrane permeable. Higher Ca²+ affinity than Fluo-4.

 

Fluo-2 is a green fluorescent, intracellular calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.

Green fluorescent, calcium (Ca²+) indicator, membrane permeable.

 

Fluo-4 is the most popular green fluorescent, intracellular calcium (Ca²+) indicator. Proven and trusted for decades to measure intracellular Ca²+ flux in high throughput screening and fluorescence microscopy applications.

Green fluorescent, calcium (Ca²+) indicator, membrane permeable.

 

Fluo-3 is a green fluorescent, intracellular calcium (Ca²+) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications. Is preferred over Fluo-4 for some cell types.

Brilliant Calcium Kits

High-throughput, no wash calcium (Ca²+) assay. An optimal solution for measuring intracellular Ca²+ dynamics, including effectors such as GPCRs and protein transporters.

ION Biosciences’ Brilliant Calcium (Ca²+) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of dynamic changes in intracellular Ca²+. The ION Biosciences’ Brilliant Calcium Assay is useful for a wide range of effectors of intracellular Ca²+ including many G protein-coupled receptors (GPCRs), divalent cation channels, and other proteins capable of transporting Ca²+ or affecting intracellular Ca²+ dynamics. In multi-well, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors which can act to affect intracellular Ca²+ levels.

Brilliant Calcium Flex Kit

Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges.


Contents of Brilliant Calcium Flex Kit
 

Green fluorescent, calcium (Ca²+) indicator, membrane impermeable

 

Fluo-3 is a green fluorescent, intracellular calcium (Ca²+) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications.Is preferred over Fluo-4 for some cell types.

 

Fluo-3 K+ salt is a membrane impermeable form of Fluo-3 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

ION Biosciences ICR-1 K+ Salt
 
ION Calcium Red – 1 (ICR-1) is a red fluorescent (Ex/Em 580nm/660nm) calcium (Ca²) indicator for intracellular Ca²+ measurements. ICR-1’s long-wavelength emission and a large Stokes shift reduces contributions of autofluorescence, making ICR-1 AM optimal for cellular and tissue imaging applications. ICR-1 can also be multiplexed with GFP-labeled cells or other green fluorophores commonly used in experiments. Unlike some other red fluorescent Ca²+ indicators, ICR-1 does not accumulate in the mitochondria. Data supporting fluorescence lifetime imaging and multiphoton imaging capabilities have also been demonstrated.
 

ICR-1 K+ salt is a membrane impermeable form of ICR-1 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Our red calcium dye, ION Calcium Red-1 (ICR-1), is a red fluorescent indicator (Ex/Em: 580/660 nm) responsive to intracellular Ca²+ dynamics. ICR-1’s spectral properties allows for multiplexing with an indicator of another color or GFP-tagged cells.

When following the recommended protocol, Red Calcium Essentials provides enough reagents to make 100 mL of working solution, enough for ten 96-well plates. The actual number of assays will vary according to optimal dye concentrations for your application.