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ActionsPall® FluoroTrans® W PVDF Transfer Membranes
- Sensitive Protein Detection with Low Background and Very Low Burnthrough
- Naturally hydrophobic polyvinylidene fluoride is ideal for a wide variety of protein-analysis applications. The family of FluoroTrans® media are white, microporous solid phase supports that bind proteins tenaciously via hydrophobic interactions.
- High sensitivity for small peptides.
- High protein binding capacity. Typically absorbs 50% more protein than nylon or nitrocellulose.
FluoroTrans® W membrane is optimized for Western transfer applications. This membrane allows for sensitive protein detection with low background and very low protein burnthrough. Immobilized proteins can be visually detected with Coomassie blue, Amido Black, Ponceau S, and colloidal gold.
This item has been discontinued.
Please contact your trusted MIDSCI Lab Consultant or Email tech@midsci.com for alternate options.
Pall® BioTrace™ NT Nitrocellulose Transfer Membranes
- Pure, Unsupported Nitrocellulose Membrane for Nucleic Acid and Protein Detection
- High binding capacity for proteins and nucleic acids
- Lower protein burnthrough than competitors in electrophoretic transfers
Pall® Biodyne®
Nylon Transfer Membranes
- High sensitivity and low background for enhanced detection and resolution.
- Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing
- Membranes are intrinsically hydrophilic for easy wetting
- Offers superior performance with radioactive (Biodyne B membrane) and non-radioactive (Biodyne A membrane) detection systems
Four Chemistries Provide Versatile Adsorption Properties:
- Biodyne A Membrane - (Amphoteric Nylon 6,6) Membrane zeta potential can be modulated by changes in pH. Ideal for single probe or multiple rehybridizations, and applications where background is troublesome.
- Biodyne B Membrane - (Positively-charged Nylon 6,6) Pore surfaces are populated by a high density of quaternary ammonium groups. Our highest sensitivity nylon membrane for nucleic acid applications.
- Biodyne C Membrane - (Negatively-charged Nylon 6,6) Can be derivatized by coupling reactions through the carboxyl groups on the pore surfaces.
- Biodyne Plus Membrane - (Positively-charged Nylon 6,6 with an extremely high isoelectric point) With certain nonradioactive detection systems, it is more sensitive than Biodyne A membrane while exhibiting lower background than Biodyne B membrane.
Pall® FluoroTrans®
PVDF Transfer Membranes
- Sensitive Protein Detection with Low Background and Very Low Burnthrough
- Naturally hydrophobic polyvinylidene fluoride is ideal for a wide variety of protein-analysis applications. The family of FluoroTrans media are white, microporous solid phase supports that bind proteins tenaciously via hydrophobic interactions.
- High sensitivity for small peptides.
- High protein binding capacity. Typically absorbs 50% more protein than nylon or nitrocellulose.
FluoroTrans PVDF membrane is optimized for N-terminal protein sequencing. The medium demonstrates good signal-to-noise ratios with standard detection systems, and immobilized proteins can be used directly for sequencing, or visually detected with common staining reagents including Amido Black, Ponceau S, and colloidal gold.