High-efficiency, large-volume culture flasks allow cells to show strong viability with large expression amounts of proteins in the culture of mammalian cells and insect cells. During the culturing process, the use rate of the shaker is significantly increased, and the survival rate and viability of cells are both dramatically elevated.
NEST culture flasks also provide high repeatability, which allows high inter-batch consistency of cell growth and yield.
Glassware performance in a contamination-free, single-use shaker flask. Manufactured from medical-grade virgin PETG which reduces gas permeability.
Nest PETG Erlenmeyer Flasks are designed for mammalian suspension culture applications as well as bacterial & yeast culture.
Seal caps prevent contamination and evaporation. Ideal for long-term storage and transporting liquids. Vent caps with 0.22 um hydrophobic membrane allow gas exchange without contamination (an oversized vent area in the cap improves gas exchange). Ideal for cell culture, fermentation, air-sensitive reactions, and preventing bacterial growth.
Glassware performance in a contamination-free, single-use shaker flask. Polycarbonate bottle meets the USP Class 6 Standard with high transparency, strong impact resistance and oxidation resistance, and can withstand temperatures up to 121ºC. There’s a scale made by injection molding on the flask body to facilitate volume observation.
Nest Polycarbonate Conical Erlenmeyer Flasks are designed for mammalian suspension culture applications as well as bacterial & yeast culture.
Seal caps prevent contamination and evaporation. Ideal for long-term storage and transporting liquids.
Vent caps with 0.22 um hydrophobic membrane allow gas exchange without contamination (an oversized vent area in the cap improves gas exchange). Ideal for cell culture, fermentation, air-sensitive reactions, and preventing bacterial growth.
Erlenmeyer Flasks
Erlenmeyer Shaker Flasks are made of polycarbonate, PETG or styrene/butadiene (Jumbo Flasks only). The polypropylene screw caps are vented with 0.2µm membranes to increase air transmission and enhance cell growth. The mouth size conforms with the international SBS standard, for quick, secure connection to bioreactor equipment.
Polycarbonate flasks and Jumbo Fernbach flasks have baffled (ribbed) bottoms to provide increased oxygenation and mixing efficiency even at lower shaking speeds. All flasks, regardless of size, are individually wrapped and gamma sterilized to SAL 10-6, and they are free of RNase, DNase, pyrogen, endotoxins and human DNA.
Polycarbonate Flasks are autoclavable at 121°C and 15PSI for 30 minutes.
NEST Transfer Cap for High Efficiency Erlenmeyer Flasks
Two-Way Liquid Transfer Cap The two-way transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. The liquid transfer is achieved by connection of a peristaltic pump between the shake flask and the device. Upon completion of the transfer, the transfer cap can be replaced with a vent cap for culture.
Multifunctional Liquid Transfer Cap Unlike the two-way liquid transfer cap, the multifunctional transfer cap can be directly placed in an incubator for culture after the liquid transfer is completed. It can reach a large air flux. The sampling part is composed of a sampling nozzle and a one-way valve, which can prevent the liquid from flowing backwards during the sampling process and ensure the aseptic sampling. The liquid inlet tubing is provided with a PTFE needle filter, which solves the issue of liquid remaining in the tubing during the feeding process.
Inverted Liquid Transfer Cap The inverted transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. When liquid transfer is required, the liquid is transferred under gravity with the inverted shake flask.
Application
It is applicable to liquid transfer and culture during mass proliferation of bacteria and suspension cells.
Product Features