PR1MA™  Luria Broth, High Salt Formula, Powder [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. High Salt Grade. Molecular Biology Grade.

• Dissolve 25 grams in 1 liter distilled water
• Adjust the pH to 7.2
• Sterilize by autoclaving for 15 minutes

• Tryptone: 10 g/L
• Yeast: 5 g/L
• Sodium Chloride: 10 g/L

• DCM Appearance: Homogeneous free flowing, light beige
• PPM Appearance: Clear,no to light ppt, light yellow
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• BSE/TSE Free: BSE/TSE Free
• Country of Origin : USA

• Appearance: White, greyish-white or yellowish-white, fine, crystalline powder or fine crystals, needles or elongated plates
• Identification: Pass
• Melting Point : 149 - 153 ^oC
• pH: 4.5 - 7.5
• Specific Optical Rotation: +17.0 - +20.0^o
• Chloride: ≤ 0.01 %
• Related Substances: ≤ 0.50 %
• Loss on Drying: ≤ 0.50 %
• Sulfated Ash: ≤ 0.10 %
• Assay (Dried Basis): 98.0 - 102.0 %
• Non Animal Origin: Non Animal Origin
• Grade: USP
• Country of Origin : Report

PR1MA™  Cycloheximide

CAS Number: 66-81-9
Molecular Weight: 281.35
Chemical Formula: C₁₅H₂₃NO₄
Solubility: Ethanol, methanol
Storage Temperature: 2-8°C

Antifungal antibiotic that inhibits protein synthesis in eukaryotes. Blocks translation of mRNA on the ribosome derived from Streptomyces griseus.


WARNING! This product can expose you to Cycloheximide, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.
 

• Appearance: White to Off White Crystalline Powder
• HPLC (Purity): ≥ 96.0 %
• Identification (HPLC): Conforms
• Melting Point: 102 - 120 ^oC
• Potency: ≥ 950 µg/mg
• Solubility: Soluble In Chloroform; Ethanol, Methanol
• Water Content : < 0.3 %

PR1MA™ Acrylamide/bis-Acrylamide, 19:1 Ratio Solution

CAS Number: 79-06-1; 110-26-9; 7732-18-5
Storage Temperature: 2-8°C

A 40% solution containing 38% (w/v) Acrylamide and 2% (w/v) bis-Acrylamide for a monomer to crosslinker ratio of 19:1.


WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear, colorless liquid
• Conductivity of Solution: ≤ 10 µmhos
• DNase/ RNase: Not Detected
• Electrophoresis: Pass Test
• pH: Report

PR1MA™ Luria Broth 1 Gram Buffered Capsules [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 77-86-1/1185-53-1
Solubility: Water
Storage Temperature: Room Temperature

High salt formula. Molecular Biology Grade. For maintenance and propagation of E. Coli.


RPI Media is now available in convenient pre-measured and pre-mixed capsules. Prebuffered media eliminates messy weighing and pH adjustments.


Simply dissolve 25 capsules per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.0 ± 0.2 at 25°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.


Ingredients:

• Tryptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L
• Tris/Tris HCl: 1.5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Growth
• Microorganism (E. coli ATCC 33694): Growth
• Microorganism (E. coli ATCC 33849): Growth
• Microorganism (E. coli ATCC 39403): Growth
• Microorganism (E. coli ATCC 47014): Growth
• Microorganism (E. coli ATCC 53868): Growth

PR1MA™ Lactobacillus MRS Broth Powder

Solubility: Water
Storage Temperature: Room Temperature


Recommended for the selective isolation and enumeration of lactobacilli.

Ingredients:

• Peptic Digest of Animal Tissue: 10.0g
• Beef Extract: 10.0g
• Yeast Extract: 5.0g
• Ammonium Citrate: 2.0g
• Dextrose: 20.0g
• Polysorbate 80:1.0g
• Sodium acetate: 5.0g
• Magnesium Sulfate: 0.1g
• Manganese Sulfate: 0.05g
• Sodium Phosphate, Dibasic: 2.0g

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing with soft lumps, yellow beige to yellow tan
• PPM Appearance: Clear, no to light ppt, medium to dark amber
• Microorganism (Lactobacillus Casei ATCC 393 ): Good to Excellent
• Microorganism (Lactobacillus Fermentum ATCC 9338 ): Good to Excellent
• Microorganism (Lactobacillus Plantarum ATCC 8014 ): Good to Excellent
• Microorganism (Lactobacillus Rhamnosus ATCC 9595 ): Good to Excellent
• pH (5.5 % Solution @25 ^oC): 6.3 - 6.7

PR1MA™ Sodium Citrate, Trisodium Salt, Dihydrate, [Citric Acid, Trisodium Dihydrate]

Common Name: Citric Acid, Trisodium Dihydrate
CAS Number: 6132-04-3
Molecular Weight: 294.1
Chemical Formula: C₆H₅Na₃O₇  2H₂O
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Assay: ≥ 99.0 %
• pH (5% Solution): 7.0 - 9.0
• Alkalinity: Pass Test
• Chloride: ≤ 50 ppm
• Heavy Metals : ≤ 10 ppm
• Identification Tests: Passes Tests
• Loss on Drying: 10.0 - 13.0 %
• Sulfate : ≤ 150 ppm
• Tertrate: Meets USP Test
• Grade: Meets current USP Specifications

PR1MA™ Chlortetracycline Hydrochloride

• Appearance: Yellow Crystalline Powder
• Crystallinity: Pass
• Identification: Pass
• Loss on Drying: ≤ 2.0 %
• pH (1% Solution in Water)@ 25°C: 2.3 - 3.3
• Potency: ≥ 900 µg/mg
• Specific Rotation: -250 - -235°

PR1MA™ Glycerol

CAS Number: 56-81-5
Molecular Weight: 92.1
Chemical Formula: C₃H₈O₃
Solubility: Water
Storage Temperature: Room Temperature

Used in sample preparation and gel formation for polyacrylamide gel electrophoresis. Also used in casting gradient gels, as a protein stabilizer and storage buffer component.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Liquid
• Chloride (Cl): ≤ 0.001 %
• Chlorinated Compounds: ≤ 0.003 %
• Color, APHA: ≤ 10.0
• Fatty Acids & Esters (USP): ≤ 1.0 (ml 0.5 N NaOH) %
• Glycerine : ≥ 99.7 %
• Heavy Metals : ≤ 5.0 ppm
• Identification C (GC): Pass
• Limit of Diethylene Glycol & RC: Pass
• Purity (USP): 99.0 - 101.0 %
• Residue on Ignition: ≤ 0.01 %
• Specific Gravity: ≥ 1.259
• Water: ≤ 5.0 %
• Date of Manufacture: Report

PR1MA™ HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]
 

Common Name: HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]
CAS Number: 16052-06-5
Molecular Weight: 252.3
Chemical Formula: C₉H₂₀N₂O₄S
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.0 %
• Loss on Drying: ≤ 1.0 %
• pH (1mol/L in Water @ 20 ^oC: 5.0 - 7.0
• Solubility (0.1M in water): Clear and Colorless

PR1MA™  Streptomycin Sulfate

• Appearance: Fine White Powder
• Appearance of Solution: Clear
• Abnormal Toxicity: Meets The Requirements
• Bacterial Endotoxins : ≤ 0.25 IU/mg
• Colorimetric: ≥ 90.0 %
• Identification: Complies
• Loss on Drying (EP): ≤ 7.0 %
• Loss on Drying (USP): ≤ 5.0 %
• Methanol (GC): ≤ 0.3 %
• pH: 4.5 - 7.0
• Potency (Dried basis): ≥ 720 µg/mg
• Residual Solvents: No Class 1, 2, 3 or other residual solvents are present
• Streptomycin B: ≤ 3.0 %
• Sulfate: 18.0 - 21.5 %
• Sulfated Ash: ≤ 1.0 %
• BSE/TSE: BSE/TSE Free

PR1MA™ IPTG [Isopropyl-β-D-thiogalactopyranoside], Dioxane Free

Common Name: Isopropyl-β-D-thiogalactopyranoside
CAS Number: 367-93-1
Molecular Weight: 238.3
Chemical Formula: C₉H₁₈O₅S
Solubility: Water, ethanol
Storage Temperature: 2-8°C

IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.


Dioxane-Free
Water Content: <0.49%

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay (HPLC): 98.0 - 101.0 %
• Purity (HPLC): ≥ 99.0 %
• 1,4-Dioxane: Not Detected
• Absorbance @300nm (5% w/v sol in H₂O): ≤ 0.13
• Absorbance @400nm (5% w/v solution in H₂O): ≤ 0.06
• Identification: IR Concordant with Standard
• Melting Range: 110 - 114 °C
• Moisture Content (KF): ≤ 1.0 %
• pH (5% in water): 5.0 - 7.0
• Solubility: Soluble in Water and Methanol
• Specific Optical Rotation: -34.5 - -28.5 ^o
• BSE/TSE Free: BSE/TSE Free
• Non Animal Origin: Non Animal Origin
• Country of Origin : Report