General Tissue Culture Supplies

MIDSCI brings you a wide selection of high-quality tissue culture supplies.

Our tissue culture supplies include flasks, dishes, tubes, and much more. Everything is right here for your cell tissue culture, cell visualization, and cell preservation and storage needs. 
  
Take a look at our tissue culture flasks, which have a uniform flat growth surface that promotes cell growth without clumping. 
  
Ensure consistent growth conditions and prevent condensation with our TPP tissue culture dishes. 
  
Grow cells using Clipmax, which includes an activated growth surface and detachable flask for delicate cells. 
  
Utilize a quick and reproducible method of cell counting based on cell volume with our packed cell volume (PCV) tubes. 
  
There’s plenty more!

Harvest Fetal Bovine Serum (FBS)

Harvest Fetal Bovine Serum (FBS)

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Harvest Premium Cell Strainers

PR1MA Premium Cell Strainers

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RephiLe Syringe Filters

RephiLe Syringe Filters

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The Same Quality You Love, Now with a Fresh New Name and Updated Item Numbers!


PR1MA Signature Cell Strainers

 

Clean, reliable filtration with every use.

 

The PR1MA Cell Strainer system is a fast, efficient, and easy-to-use solution designed to streamline the process of cell isolation and filtration. Ideal for flow cytometry, this advanced straining system is perfect for detaching, isolating, and filtering cells from clusters and tissue samples.


Each cell strainer features a durable polypropylene frame supporting a nylon mesh, available in eight distinct, color-coded pore sizes ranging from 5 uM to
100 uM. Eight fine mesh options offer enhanced control for filtering cell aggregates and clumps, improving experimental precision.

 

The innovative frame design significantly improves filtration performance. By eliminating the “air-lock” effect, PR1MA Signature Cell Strainers promote faster flow-through and better aeration, reducing spillover and processing time.

 

Key Features

  • Available in 8 color-coded pore sizes (5 uM to 100 uM) for precise cell sorting
  • Fast flow-through thanks to advanced polypropylene frame design
  • Includes universal adapter for tubes smaller than 50 mL
  • Compatible with 6-well plates, flat-bottom plates, and culture dishes
  • Interlocking and stackable design for improved lab workflow
  • Built-in 1.5 mm feet for elevated straining into flat vessels
  • Supplied sterile in individual blister packaging
  • Certified RNase-, DNase-, pyrogen-, and cytotoxin-free

Specifications

Item #SizeColorSterilityDescriptionPackaging
PR-CS5-1S5 uMOrangeSterilePP frame, nylon meshIndiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS10-1S10 uMTealSterilePP frame, nylon meshIndiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS20-1S20 uMRedSterilePP frame, nylon meshIndiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS30-1S30 uMPurpleSterilePP frame, nylon meshIndiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS40-1S40 uMBlueSterilePP frame, nylon meshIndiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS50-1S50 uMGreySterilePP frame, nylon meshIndiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS70-1S70 uMWhiteSterilePP frame, nylon meshIndiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS100-1S100 uMYellowSterilePP frame, nylon meshIndiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
CELLADAPTPR1MA™ Signature Reducing Adapter or 15 / 5 mL, 12 x 75 mm & 100 mm tubes, 25/pk
  

Item#:
ASCELLSTAIN1


PR1MA Signature Double-Sided Pestles

 

Sterile, Disposable Tool for Cell Prep and Sample Homogenization.

 

The PR1MA Signature Double-Sided Pestle is a high-performance, disposable laboratory tool designed to streamline sample preparation, cell homogenization, and resuspension tasks. Made from durable polypropylene, this 145 mm pestle features a dual-ended design for enhanced versatility in biological workflows.

 

The conical end is perfect for grinding soft biological tissues, homogenizing cells, and resuspending pellets in 1.5 mL microcentrifuge tubes.

 

The convex end (11 mm diameter) with a dimpled surface is optimized for cell manipulation within disposable strainers, improving cell sorting, flow cytometry prep, and overall cell viability.

 

Ideal for researchers working in cell biology, immunology, and molecular biology, this pestle is gamma-sterilized, RNase/DNase-free, and individually wrapped for contamination-free use.

 

Key Features

  • Dual-purpose pestle with conical and convex ends
    • Conical end for grinding, homogenizing, and resuspending in 1.5 mL tubes
    • Convex end with textured surface for optimal use with cell strainers
  • Improves cell prep for flow cytometry, enhancing yield, viability, and purity
  • Gamma-sterilized, RNase-/DNase-free, and individually packaged
  • 145 mm length for comfortable, ergonomic handling
  • Packaged in packs of 50, ready for sterile use

Applications

  • Cell sorting and preparation
  • Sample homogenization
  • Pellet resuspension in microcentrifuge tubes
  • Flow cytometry sample prep
  • Nucleic acid–sensitive workflows   

Item#:
ASPRPESTLE
Cell Culture Inserts


PR1MA Plates-Permeable Cell Culture Inserts

  • Permeable Cell Culture Inserts mimic in vivo conditions
  • Inserts center in wells, preventing membrane from touching sides and wicking between insert and side wall
  • Membrane remains suspended above well bottom, allowing co-culture: grow one cell line inside insert, and another below in the well
  • Ideal for drug transfer assays, transport studies, migration assays, and cell-cell interaction studies
  • Transfer cultures to fresh media by moving the insert to a new plate
  • 0.4 uM pore size is ideal for co-culture and drug transport studies
  • 3.0 uM and 8.0 uM pore size are ideal for angiogenesis and chemotaxis experiments
  • For more help with membrane selection, please contact CELLTREAT Scientific Products
  • Non-Treated Plates sold separately; carrier plate is Tissue Culture Treated which encourages cell adhesion

Specifications 

SterileYes
Non-pyrogenicYes
AutoclavableNo
Primary ColorMembrane: Natural
Secondary ColorPlate: Clear
Secondary MaterialPlate: Polystyrene
Tissue Culture TreatedYes
RNase/DNase FreeYes
Item#:
ASPERMINSERTS

Glass Bottom Plates

  • Convenient live cell viewing without needing to transfer
  • Optically clear borosilicate glass for exceptional viewing
  • Applications include: cell culture and imaging, live cell observation and specialty microscopy such as confocal high resolution and contrast
  • Constructed with noncytotoxic medical adhesive
  • Round cover glass also available for use with glass bottom plates


Glass Bottom Dish

  • Recessed, consistent borosilicate glass bottom is optically clear, and provides micro well ideal for cell culture and microscopy or imaging
  • Non-cytotoxic medical adhesive does not inhibit cell growth
  • 30mm Dish lid has molded spacers to allow gas exchange and a stacking ring for increased stability


Cover Glass

  • High-quality, optically clear borosilicate glass cover slips are ideal for specialty microscopy and imaging
  • Ideal for use with dishes and multiple well plates and anywhere a square cover glass is unsuitable
  • Consistent flatness and scratch-free
  • Resistant to organic solvents
  • Packaged in a hinged plastic storage case
  • 15mm Round Cover Glass can be used with a 24 well glass bottom plate (Part No. 229125)
  • 20mm Round Cover Glass can be used with a 12 well plate or 30mm glass bottom dish (Part No. 229632)
  • 25mm Round Cover Glass can be used with a 6 well glass bottom plate (Part No. 229107)
  • Cover Glass is #1.5 thickness
Item#:
ASGLASSMISC

Chambered Cell Culture Slides

  • Cells grow directly on the slide without any need for future transfer
  • Ideal for growing and viewing cells under a microscope on a standard 25mm x 75mm slide
  • Uniform hydrophylic surface is ideal for consistent cell attachment
  • Slides are labeled with numeric markings
  • Packing trays can be used as incubation racks in CO2 incubators
  • Adhesive free
Item#:
ASCHAMSLID1


PR1MA™ Centrifuge Tube Filter Systems

  • Eliminate a sample transfer step by filtering directly into a 50mL tube
  • Polyethersulfone (PES) membrane for high flow and low binding
  • One Centrifuge Tube Stand included with each case of 50mL Centrifuge Tube Filter Systems
  • Centrifuge tube Stands are manufactured from polypropylene and are autoclavable, reusable and recyclable
  • Centrifuge Tube Stand will hold one 50mL tube, three 15mL tubes and three microcentrifuge tubes (1.5mL)


Specifications
 

Item667709-B667710-B
Pack Description1/Bag1/Bag
Case Quantity1212
SterileYesYes
Non-pyrogenicYesYes
Primary ColorClearClear
Secondary ColorCap: BlueCap: Blue
Primary MaterialTube: PolypropyleneTube: Polypropylene
Secondary MaterialFilter: PolystyreneFilter: Polystyrene
Maximum Volume50 mL50 mL
Working Volume50 mL50 mL
GraduatedYesYes
Graduation Increments
(Subdivisions)
2.5 mL2.5 mL
Max Force (RCF)g16,00016000
Pore Size0.45 µm0.22 µm
RNase/DNase FreeYesYes
Membrane Diameter50 mm50 mm

 

 

Item#:
ASCENTTUBFILSYS

 
PR1MA™ Cell Strainers

 

PR1MA™ cell strainers from MIDSCI™ can be used for many applications including isolating cells and removing clumps or debris. They are compatible with standard 50 mL centrifuge tubes. The exclusive Pestle design improves the manipulation of material in the strainer during operation.


  • Polypropylene housing with nylon mesh filter eliminates the need to fabricate filters from gauze, paper, nylon mesh, or other materials
  • The elongated lip on the housing extends beyond the edge of the centrifuge tube enabling aseptic handling with forceps
  • Extended gripping tab on housing for easy handling
  • Available in three porosities: 40, 70 & 100 µm
  • Color-coded by mesh porosity for easy identification
  • Packaged sterile (gamma irradiated) in individually wrapped peel-to-open paper/plastic wrapper or bulk packed in bags

 

 

Item#:
ASCELLSTAIN2


Harvest Fetal Bovine Serum (FBS)


Fetal Bovine Serum is collected entirely from USDA-licensed abattoirs and processed within the United States.

  • High-quality FBS at an affordable price
  • Store at -20ºC 
  • Low Endotoxin lots (< 1.0 EU / mL) may be available. 
  • From USDA-licensed facilities inside and outside the U.S.
  • Tested for Growth Promotion*
  • Large lot sizes
  • Virus Screened-Mycoplasma Tested
  • Triple 0.1 uM Filtered
  • Endotoxin <  2.0 EU / mL
  • Low Hemoglobin lots (< 15 mg / dL) may be available.

Specifications

Item #Description
USFBSUS Origin, Fetal Bovine Serum (FBS)
USFBSHIUS Origin, Fetal Bovine Serum (FBS), Heat Inactivated
USDAFBSUSDA Certified, Fetal Bovine Serum (FBS)
USDAFBSHIUSDA Certified, Fetal Bovine Serum (FBS), Heat Inactivated

Proudly made in the USA! Click here for more made in America products!
 
 

Item#:
ASFBS

NEST Cell Culture Inserts

  • Innovative hanging design facilitates pipetting.
  • Supplied in multiple well plates: 6-well, 12-well, 24well.
  • PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
  • Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
  • Transparent PET membrane is easy to observe.
  • The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
  • Compatible with most solvents used for fixing and dyeing.


Attention

  • Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
  • During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
  • Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.


Guidelines

  • Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
  • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
  • Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
  • Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
  • Check the volume of the culture medium regularly and add fresh medium as needed.
  • The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
  • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
  • Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.


Material

Multiple well plate or dish is made from PS.


Application

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produces a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.

Item#:
ASCELINSERT

10 Layer BioFactory™ Culture Chambers with Tube 

10 layers Biofactory™ with tube With ISO9001&13485 certified, 10 layers BioFactory™ with tube are manufactured in 100,000 grade cleaning room with USP 6 grade raw materials. Nest is committed to be your best choice for laboratory and medical consumables.

Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof. In addition to this product, NEST has launched over 200 plastic consumable products for Cell culture, Molecular Biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments.

Product Introduction
The system of Biofactory 10 Chambers (with tube) realizes the closed transfer of liquid, which can directly transfer the culture medium or cells to a cell factory or a bioreactor through a connecting tube in a sterile environment, greatly reducing the risk of exogenous pollution in the drug development and production process, simultaneously omitting the operations of tube design, assembling and sterilization, therefore, improving the production efficiency.

Product Characteristics
Pre-assembled radiation-resistant high throughput air filter, pervious and resistant to bacteria, ensuring sterility and high flow rate.
Closed liquid transfer, avoiding open operation and reducing the risk of pollution in the process of liquid transfer.
The liquid inlet tube can be aseptically welded under a normal environment
High-quality materials and smooth inner wall of the tube, ensuring an excellent transmission performance
Electron beam sterilization, SAL = 10-6
No endotoxin and no components of animal origin
Nest BioFactory™ Systems are compact, multi-layer, single-use cell culture systems designed for easy scale-up cell culture applications, such as production of vaccines, monoclonal antibodies or pharmaceuticals. Versatile, easy-to-use systems for small scale research or commercial production with reduced contamination risk. Narrow mouth caps available for tubing solutions.

Product Description

Cat.No.
Product Name
Tube Information
Tube Length
/Case
C71554-BZD080A
Biofactory 10 Chambers (With Tube)
Thermoplastic Hose, Hose Diameter: 1/4" Id, 3/8" Od
80 cm
2
 
Item#:
AS10LAYERBIOFCULCH

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

  • Choose britelite plus:
    • Half-life = 30 min
    • Brightest signal = when looking for maximum sensitivity
    • Shortest half-life = for continuous processing of samples
  • Choose neolite:
    • Half-life = 150 min
    • High signal intensity, longer lasting signal = good balance
    • For both continuous and batch processing of samples
    • Best robustness (less dependent on mixing quality)
    • Less sensitive to phenol red
    • Can be stored 1 month at 22°C
  • Choose steadylite plus:
    • Half-life = 240 min
    • Longest signal half-life = for batch processing of samples
    • Super-sensitivity not needed
Item#:
ASLUMASSAY

5-Layer Polystyrene Cell Culture Flask

 
Description
  • Nest Multi-Layer Flasks provide 870 cm2 cell growth surface area. The tissue culture treated NEST Multi-layer Flasks enable you to grow more cells faster and easier, thereby making your cell culture workflow more productive, easier, and in a more convenient manner.
 
Product features
  • Vacuum plasma TC treatment for the best cell attachment
  • Sterilized by E-Beam
  • Non-Pyrogenic, DNase/RNase FREE
  • Both plug seal cap and vent cap are available
  • Growth area: 870cm2
  • TC treatment for better cell attachment
  • Sterilized by E-Beam, non-pyrogenic
  • Tips: To avoid foams and bubbles causing the media in upper layer flowing into the bottom layer, users should be gently when operating the multi-layer flasks.
Item#:
AS5LAYERPOLCECUFK
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