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Home > PCR, Protein, RNA, DNA > PCR > Reagents > PCR Reagents > PREMIUM Bullseye Taq DNA Polymerase Master Mix

PREMIUM Bullseye Taq DNA Polymerase Master Mix

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Item #: ASPCRREAG3

Suggested Protocol using Taq 2x Mix

This protocol serves as a guideline for primer extensions. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.

Notes:

Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis. Work on ice at all times.

The final MgCl2 concentration of this 2x Master Mix is 1.5 mM. If more than 1.5 mM MgCl2 is required, use 25 mM MgCl2 (may be purchased separately) to adjust the Mg2+

Additional volume (µl) of MgCl2 per 50 µl reaction

Final MgCl2 conc. in reaction (mM)	1.5	2	2.5	3	3.5	4	4.5
Additional volume of 25 mM MgCl2 per reaction (µL):	0	1	2	3	4	5	6

Thaw Taq 2 Master Mix and primers. It is important to thaw the solutions completely and mix thoroughly before use to avoid localized concentrations of salt. Keep all components on ice.

The table below shows the reaction set up for a final volume of 50 m If desired, the reaction size may be scaled down. Use 10 µl of the Taq 2x Master Mix in a final volume of 20 µl.

Important: Spin Taq Master Mix vials briefly before use.

Set up each reaction as follows:

Component	Vol./reaction	Final Conc.
Taq 2x Master Mix	25 µL	1X
25 mM MgCl2	0 µl (0-7 µl)	1.5 mM (1.5-5 mM)
Primer A	1 µL (0.5-5 µl)	0.2 µM (0.1–1.0 µM)
Primer B	1 µL (0.5-5 µl)	0.2 µM (0.1–1.0 µM)
Distilled Water	Variable	– – – –
Template DNA	Variable	Genomic DNA 50 ng (10-500 ng) Plasmid DNA 0.5 ng (0.1-1 ng) Bacterial DNA 5 ng (1-10 ng)
TOTAL volume	50 µL	– – – –

Mix gently by pipetting the solution up and down a few times.

Program the thermal cycler according to the manufacturer´s instructions. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

Place the tubes in the thermal cycler and start the reaction.

Three- step PCR Program:

Cycles	Duration of Cycles	Temperature
1	2-5 minutes^a	95°C
25-35	20-30 seconds^b 20-40 seconds^c 30 seconds^d	95°C 50-65°C 72°C
1	5 minutes^e	72°C

^a Initial denaturation step. Optional, but recommended for genomic DNA

^b Denaturation step: Heating the DNA template disrupts the hydrogen bonds yielding ssDNA.

^c Annealing step: Primers anneal to ssDNA template. Typically annealing temperature is 3-5°C below the Tm of the primers.

^d Extension/elongation step: Taq DNA polymerase synthesizes a new DNA strand complementary to the DNA template. Extension time depends on length of DNA fragment to be amplified. At optimum temperature the DNA polymerase will polymerize 1000 bases per minute.

^e Final elongation step: After the last PCR cycle to ensure that any remaining ssDNA is fully extended.

Aspire Aspirator

Compact Design
Built-in Vacuum Pump
Adjustable Vacuum Pressure
Vacuum Gauge
Safe and Precise Aspiration

The Accuris ASPIRE Laboratory Aspirator incorporates a quiet, maintenance-free pump inside a sturdy, yet compact base. The vacuum is fully adjustable, with a pressure gauge that displays the vacuum level, allowing full control of aspirating speed. The ASPIRE is an ideal choice for a wide range of laboratory aspirating applications, including removal of media from tissue culture vessels or plates, and supernatants. An ergonomic, hand held aspirating device is included, and holds standard 200 to 300ul disposable pipette tips.

Comes complete with:

Base with internal vacuum pump
2 Liter, Polycarbonate Collection Bottle (autoclavable and break resistant)
Antibacterial / Hydrophobic Filter
Silicone Tubing
Handheld Vacuum Controller
2 Pipette cone adapters (for 200 ul pipette tips and 40mm stainless steel)

The collection bottle incorporates a reliable Overflow Protection Valve that closes off the tube to the vacuum in the event of a full bottle. In addition, the replaceable in-line filter protects the vacuum pump from contaminants and any moisture that comes through the bottle. Graduations on the collection bottle indicate the amount of fluid collected and when it's ready to be emptied. The Handheld Vacuum Pipette has an ergonomic design that's comfortable in the hand during prolonged use. Pressing the button applies the vacuum. The pipette accepts Pasteur pipettes, serological pipettes, and also comes with 2 accessory pipette cones. One cone is designed to accommodate 200/300ul pipette tips, the other has a thin, stainless steel capillary for fine control aspirating.

Specifications

Max. Aspiration	14 mL/sec
Max. Flow	15 L/ min.
Vacuum Range	-30 to -750 mbar
Noise Level	59.6 db
Vacuum Pump	oil free piston
Dimensions	9.5 x 7.9 x 13.5 in.
Weight	4.8 lbs
Warranty	2 years

Item #	Description
V0020	115 V ASPIRE Aspirator
V0020-E	230 V ASPIRE Aspirator
V0020-1SL	Extra Long Single Channel Stainless Steel Adapter
V1002-8	Eight channel adapter for 200 µl pipette tips
V1002-S8	Eight channel adapter, fine tip, stainless steel

SKU Number	Vol	Magnesium	Dye	Enzymes	Pkg	Type	Price	Quantity	Add to Cart
BE140303	500 runs	Mg	No Dye	Premium	Mix	Standard	350.4900 350.49 Each
BE180303	500 runs	Mg	Red Dye	Premium	Mix	Standard	297.8800 297.88 Each		Item is no longer available.