ENDURO VE20 Vertical Gel Electrophoresis System

Enduro VE20 Vertical Electrophoresis System (20 x 20 cm), includes glass plates, 2 x 24 well combs, cooling coil and casting base

• Large format vertical gel system
• Electroblotting and gel electrophoresis in same universal gel tank
• Quick assembly system for ease of use
• Complete system allows casting of gels and running of gels at same time increasing efficiency

The ENDURO VE20 is Labnet's new large format vertical gel electrophoresis system. It distinguishes itself in its flexibility of applications and ease of gel casting and set-up requiring only 4 screws to secure 4 gels. The same gel tank can house a large format electroblotting assembly sold separately. This system is ideal for first- and second dimension SDS-PAGE, native, preparative, gradient and high-resolution nucleic acid electrophoresis.

Innovative screw-clamp technology consists of only four screws to secure up to four 20 x 20cm gels. This vertical screw clamp technology distributes pressure evenly along the height of gel rather than in the center to eliminate plate bowing and gel compression, while maintaining a leak-proof seal during casting.

NO TANK TOP ASSEMBLY
A built-in inner buffer chamber within the PAGE insert allows set up to be completed without inclusion of an upper buffer chamber.

CASTING ADVANTAGES

• Dual purpose PAGE insert eliminates time consuming transfer of glass plates between separate casting and gel running components.
• Glass plates with bonded spacers assure clean well formation, correct alignment for leak-free casting and eliminates manually aligning spacers.
• Casting stand design ensures leak-free casting even if glass plates are misaligned.

Specifications:

ENDURO™ Semi Dry Blotter

• Western, Southern, and Northern Blots
• Rapid transfer times
• Uniform head dispersion
• Variable gel thickness
• Minimal buffer volume

The ENDURO™ Semi-Dry Blotter provides great flexibility in a lab since it can be used for all types of blotting: Western, Southern, and Northern. Blotting with the ENDURO™ Semi Dry Blotter typically can be done in 15 to 30 minutes making it a fast and easy way to do all your blotting applications. Set up procedures are easy and economical with little buffer needed. The screw down lid adjusts to varying gel thicknesses and sizes while the platinum coated electrodes provide uniform pressure ensuring even transfers. To provide your lab with total blotting solutions use with our ENDURO™ 250V, 3000mA high current power supply.

Specifications

Semi-Dry Blotting Systems, 10cm x 10cm

Accuris MyVolt™ Mini Power Supply

• Compact and simple
• Choose constant 100 V or 200 V output
• Two pairs of output terminals

The Accuris MyVolt Mini is a compact electrophoresis power supply ideal for basic, constant voltage applications. Users can select constant 100 V or 200 V output, and the current can reach a maximum of 200 mA.

Two sets of output jacks are available to connect up to 2 gel rigs and these small power supplies can securely be stacked for use with additional gel rigs.

Specifications

Wide Format Gel System 23.5 cm x 14 cm

•     Heavy duty lower buffer chamber
•     Interlock safety lid with attached leads
•     Non-gasketed 23cm wide x 14cm long UV-transparent gel tray with built-in fluorescent ruler and 4 comb slots.
•     Four 1.5mm x 50 tooth combs
•     Opaque well visualization plate.

PRIME G (gradient) Thermal Cycler

• Colour touchscreen for fast program setup
• Small space-saving footprint
• Temp Range 4°C to 100°C
• Fast ramp rate of up to 3.0°C/sec
• Larger block sizes; 48 x 0.2ml, 30 x 0.5ml and half a 96 well plate in horizontal format.
• Gradient can be applied across a Temp Range of 30°C to 80°C
• Maximum Gradient 30°C
• Gradient calculator function
• Data transfer via USB
• 4 year warranty

The 3 PRIME G is a small gradient thermal cycler that builds on all the features of the 3 PRIME X instrument. The 48 well block format offers eight columns for annealing temperature optimisation and six rows for optimising reagents such as MgCl2 and primer concentrations. Annealing temperatures can be optimised over 30oC between temperatures 30°C to 80°C. The gradient calculator function displays the temperature for each of the eight columns, ensuring easy replication of thermal conditions.

Technical Specification

Please know that the manufacturer has suspended manufacturing this product indefinitely. 

Contact your trusted MIDSCI Lab Consultant
or email tech@midsci.com for more information.

Key benefits
Speed

• 40 cycles in 40 minutes before fast optimization.
  
• 40 cycles in 15 minutes when optimized.
  
• Genotype in 4 minutes with over 99% accuracy.
  
• Set up a 4plex reaction in only 4 mouse clicks.
  
  Confidence
  
• MIQE compliant - always consistent, always compliant.
  
• Instant settle time to achieve world leading uniformity of ±0.1°C across the block - any well, any instrument - consistent results in any system.
  
• 400 specific qPCR reagent kits available.
  
  
  Performance
  
• Consistent results provide trust and confidence. Well-to-well, run-to-run , machine-to-machine.
  
• Compile data from multiple experiments. Pool plates and results across labs and instruments.
  
• Record every well, with every filter, in every cycle. Never miss a data point.
  
• Adaptive LED Control means you never have uncertainty of saturated unknowns, no bleaching though light to neighboring wells or maximizing the wide dynamic range. Trust results are correct. 
  
• All other block-based qPCR machines have lower uniformity. If you perform Next Generation Sequencing, any other instrument than Prime Pro 48 is risking your NGS, with significant cost implications.
  
  Value
  
• No other qPCR system offers so much performance for such a low price.
  
• Available as a complete system - Techne instrument, plates, seals and reagents.
  
• Alternatively, is chemically open platform - use your preferred supplier reagents.
  
• Lowest price per run - use duplicates and low sample volumes. Don't waste money with triplicates and large sample volumes.
  
• Everything as standard - no add-ons, HRM as standard, unlimited software license. 
  
• Highly accurate and uniform qPCR prevents means successful NGS runs and significant moneysaving.
  
  Sensitivity
  
• ±0.1°C uniformity across the whole block instantly after every temperature change means that any well, in any instrument, provides same answer
  
• HRM - Identify Class IV SNP >99.9% of the time
  
• Unsurpassed block uniformity with patented block technology enables the researcher to use duplicates on Prime Pro 48, whereas triplicates are required on less uniform competitor machines.

   

  The Prime Pro 48 real time PCR system is a new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.
  
  High thermal uniformity and validated sample volumes down to 5ul ensures Prime Pro 48 allows researchers to bring run times for an optimized 40 cycle reaction down to only 15 minutes. PCR efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the plate, ensuring that all samples are processed equally. The unique thermal block design of Prime Pro 48 achieves this with a
  unique heating and cooling system that quickly cycles from one temperature to the next and then achieves a uniform ±0.1°C in every well of the block within a fraction of a second of reaching each dwell temperature. No other block-based qPCR system can achieve similar performance.
  
  The capacity of Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity.
  
  Technical Specification

  High Resolution Melt	Yes
  Volume per well	Validated for 5 to 20 ul
  Detection sensitivity	1 copy
  Temp uniformity	+- 0.1 °C within 1 sec
  Temp stability	+- 0.1 °C
  Temperature range	30 to 100 °C
  Average ramp rate	5.5 °C sec
  Thermal system	Hollow silver block peltier-based with conductive fluid
  Block format	48-well block
  Consumables	48-well custom plates and optical adhesive seals
  Optical system	Dual LED excitation (452 to 486 nm and 542 to 582 nm)
  Camera	CCD camera
  Filters	4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm)
  Calibrated dyes	SYBR FAM HEX ROX Cy5
  Additional dyes	All dyes within range compatible with no additional calibration
  Passive reference dyes	Use of ROX is supported but optional
  Data collection	Data always collected in all four filters for all wells
  Plate setup	Plate setup for data analysis can be altered after run completes
  Melt curve	Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times
  PCR cycle time	40 cycles in less than 40 minutes
  PCR cycle time (FAST optimized)	40 cycles in less than 20 minutes
  Dynamic range	Greater than 9 logs
  Calibration	Not required
  Installation	Plug and play design
  Precision	Discriminates 5000 and 10000 template copies with 99 percent confidence
  Warranty	1 year (parts and labor included)
  Voltage	120 to 240 V
  Frequency	50 and 60 Hz
  Nominal current draw	8A
  Peak power	500W (typical power is 180W)
  Software	Multiple license software included at no additional cost
  Chemistry	All chemistries supported
  Applications	Absolute Quantification and Relative Quantification
  Applications	Allelic Discrimination and High Resolution Melt
  Dimensions closed (WxDxH)	34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches)
  Dimensions open (WxDxH)	34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches)
  Weight	13.6 Kg (30 lb)

   

   

  Ordering information

  Part number	Description
  PRIMEPRO48	Prime Pro 48 Real Time PCR System, includes Accessories and Software (Pro and ProControl). PC not supplied
  PROPLATE48	Pack of 50 Real Time PCR Plates, 48-wells
  PROSEAL48	Pack of 50 Real Time PCR Plate seals

PRIME Base Thermal Cycler

• Colour touchscreen for fast program setup.
• Temp Range 4°C to 100°C
• Fast ramp rate of up to 3.4°C/sec
• Can be upgraded for gradient cycling
• Data transfer via USB
• 4 year warranty

This full sized thermal cycler delivers both high performance and high throughput to provide maximum flexibility when processing a large number of samples in parallel. 


User friendly programming is achieved via a colour touchscreen and intuitive software that is standardised across the entire Techne thermal cycler range. A USB port enables the transfer of programmes between instruments and temperature logs to your PC. A real time graphical display provides an instant visualisation of the program status. 

Gradient cycling can be achieved directly using the PRIME G instrument or by upgrading the PRIME BASE unit. This enables the laboratory to manage changing cycling requirements in a cost effective manner.

The Techne PRIME cycler range is one of the most affordable full sized thermal cyclers on the market and is backed up by a 4 year warranty.

Technical Specification

Quick View

5 Prime G gradient thermal cycler

Please know that the manufacturer has suspended manufacturing this product indefinitely. 
 
Contact your trusted MIDSCI Lab Consultant
 or email tech@midsci.com for more information.

PRIME G (gradient) Thermal Cycler

• Colour touchscreen for fast program setup.
• Versatile block format
• Temperature range 4°C to 100°C
• Fast ramp rate of up to 3.4°C/sec
• Gradient can be applied across a temperature range 30°C to 80°C
• Maximum Gradient 30°C
• Gradient calculator function
• Data transfer via USB
• 4 year warranty

The PRIME G is a gradient enabled thermal cycler with all the features of the PRIME BASE unit. The wide linear gradient with a range of 30°C allows annealing temperatures to be optimised in one experiment. The gradient calculator function displays the temperature for each of the eight columns, ensuring easy replication of thermal conditions.

Technical Specification

• Fast ramping - up to 5°C/second
• Intuitive graphical programming
• Lightweight and compact
• Interchangeable blocks - 24 x 0.2 ml and 18 x 0.5 ml
• Economical - half the price of a full size unit
• 18 month warranty